FastQCFastQC Report
Sat 18 Jun 2016
SRR3553809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614383
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336012.0813524423882064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38520.2386050893747023No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC31740.19660762037261295No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC31700.19635984769413453No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC29290.18143154381581073No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG23600.14618588030225788No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG20080.1243818845961584No Hit
CGTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGT18680.11570984084941428No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT16850.10437424080902734No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAC200.001255919340.00000429
CGCGTAG304.86874E-640.0000041
CGTTTTT176500.039.2181321
ACACGAC4550.038.2417626
CGTTATT9050.036.685081
CTATCGG503.426976E-936.0000042
CGGTCTA503.426976E-936.00000431
GACACGA4800.035.83333625
CTATACG455.444781E-835.5555571
CACGACC4900.035.51020427
CGAGACA5250.034.66666822
GTTTTTT201100.034.559922
CGGGTAT2200.034.5454526
ACGGGCT5050.034.4554445
GTACGGG5600.034.2857133
ACGACCA5100.034.11764528
ACTACGG1350.034.0740742
GCGAGAC5350.034.01869221
TCGAACG653.45608E-1133.8461531
ACGGGAC6250.033.65
Produced by FastQC (version 0.11.5)