##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553809_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1614383
Sequences flagged as poor quality	0
Sequence length	46
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.597414615986416	31.0	31.0	34.0	30.0	34.0
2	31.801156850635817	31.0	31.0	34.0	30.0	34.0
3	31.426517127596117	31.0	31.0	34.0	28.0	34.0
4	35.395396259747535	37.0	35.0	37.0	33.0	37.0
5	33.617906655359974	37.0	35.0	37.0	30.0	37.0
6	34.44960024975486	37.0	35.0	37.0	30.0	37.0
7	35.46237478962551	37.0	35.0	37.0	32.0	37.0
8	35.57889422770185	37.0	35.0	37.0	35.0	37.0
9	37.3272216072642	39.0	37.0	39.0	34.0	39.0
10	36.74641519391619	39.0	37.0	39.0	32.0	39.0
11	36.53514376699953	39.0	35.0	39.0	32.0	39.0
12	36.79442053093968	39.0	37.0	39.0	32.0	39.0
13	33.37507456409043	38.0	35.0	39.0	15.0	39.0
14	35.61246742563568	39.0	35.0	41.0	18.0	41.0
15	36.95151460341195	39.0	35.0	41.0	30.0	41.0
16	37.507265004648836	39.0	36.0	41.0	32.0	41.0
17	37.666066850307516	39.0	37.0	41.0	32.0	41.0
18	37.6451610305609	39.0	37.0	40.0	33.0	41.0
19	37.51766154623779	39.0	37.0	40.0	33.0	41.0
20	37.37522198883413	39.0	35.0	40.0	32.0	41.0
21	37.27080624610145	39.0	35.0	40.0	32.0	41.0
22	37.2604090850808	39.0	35.0	40.0	32.0	41.0
23	37.124126678737326	39.0	35.0	40.0	32.0	41.0
24	37.05405718469533	39.0	35.0	40.0	32.0	41.0
25	36.8550907684236	39.0	35.0	40.0	31.0	41.0
26	36.83019642798518	39.0	35.0	40.0	32.0	41.0
27	36.46606102764957	38.0	35.0	40.0	31.0	41.0
28	36.47142097011675	38.0	35.0	40.0	31.0	41.0
29	36.49838792901065	38.0	35.0	40.0	31.0	41.0
30	35.97194903563776	38.0	35.0	40.0	30.0	41.0
31	35.92678936782659	38.0	35.0	40.0	29.0	41.0
32	35.652187863722546	38.0	35.0	40.0	27.0	41.0
33	35.31809675894753	38.0	34.0	40.0	25.0	41.0
34	35.048538048282225	38.0	34.0	40.0	23.0	41.0
35	34.82133979359297	38.0	34.0	40.0	22.0	41.0
36	34.624004960409025	38.0	34.0	40.0	21.0	41.0
37	34.532902043690996	38.0	34.0	40.0	21.0	41.0
38	34.39602808007765	38.0	34.0	40.0	20.0	41.0
39	34.28846128830643	38.0	33.0	40.0	19.0	41.0
40	34.206771255643794	38.0	33.0	40.0	18.0	41.0
41	34.00704355781745	38.0	33.0	40.0	18.0	41.0
42	33.896953201315924	38.0	33.0	40.0	18.0	41.0
43	33.77143651785233	38.0	33.0	40.0	17.0	41.0
44	33.694011891849705	38.0	33.0	40.0	17.0	41.0
45	33.642029803336634	37.0	33.0	40.0	18.0	41.0
46	32.63214305403365	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	15.0
10	33.0
11	33.0
12	20.0
13	24.0
14	36.0
15	60.0
16	127.0
17	261.0
18	536.0
19	1080.0
20	1972.0
21	3174.0
22	5084.0
23	7787.0
24	11671.0
25	17322.0
26	25568.0
27	36450.0
28	44413.0
29	46599.0
30	47635.0
31	53386.0
32	63302.0
33	79568.0
34	108195.0
35	137820.0
36	166291.0
37	220998.0
38	281936.0
39	252979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.57002706297081	22.272471897932522	20.91529705156707	30.242203987529603
2	34.382113785886006	23.82619242150097	19.759933051822276	22.031760740790755
3	24.451632605150078	24.363487474781387	30.651090850188584	20.533789069879948
4	22.30678841390178	24.878792702846848	30.23433720498791	22.58008167826346
5	18.99722680429613	32.00027502767311	27.21671375379944	21.78578441423132
6	18.791885197007154	34.839502150357134	29.235069992684508	17.1335426599512
7	75.4892116678632	4.468394426849143	16.24459623274031	3.797797672547345
8	75.47707080661776	4.074807527086199	16.206872842441975	4.241248823854067
9	68.45729916630688	6.914963797314516	18.84794376551289	5.779793270865712
10	27.992551953284938	30.0222437922104	25.537000823224727	16.448203431279936
11	22.84544621691383	24.34143570639681	35.11595451636941	17.697163560319947
12	19.598818867641693	22.287152429132366	38.29202859544482	19.822000107781115
13	18.582641170032144	28.032133638671862	36.60190921237402	16.78331597892198
14	18.808424023295586	25.557442069199194	36.603333905275264	19.030800002229952
15	18.257935074886195	24.90338414118583	38.382651452598296	18.456029331329677
16	20.112141914279324	24.872288670036788	36.49809246009156	18.517476955592322
17	19.59293426652783	24.93751482764623	35.016535729129956	20.45301517669599
18	20.94515365932372	24.040825504232888	35.35666567351118	19.657355162932216
19	21.26583344844439	25.739616931050442	33.84376569872205	19.15078392178312
20	23.1237568780147	24.248025406610452	32.97259696119198	19.655620754182866
21	23.122332185113446	25.445448818526955	33.43425940436687	17.99795959199273
22	22.10256178366596	23.00482599234506	33.49459205157636	21.398020172412618
23	19.99841425485774	25.53198342648554	34.28442940739589	20.185172911260835
24	19.498161217009844	24.494063676339508	35.344834528113836	20.662940578536816
25	21.027352245408927	25.557256239690336	33.20067171173135	20.214719803169384
26	19.8307960378671	25.290528951308332	33.94659136029059	20.93208365053398
27	21.061978477226283	24.356116237596652	33.389040890544564	21.1928643946325
28	20.007891559809536	25.079488572414355	34.07853031158034	20.834089556195774
29	21.12330221514969	24.183852282884544	32.94633305727327	21.746512444692492
30	21.688533637928543	23.813184355880853	33.21813968556408	21.28014232062652
31	21.658181484814943	23.570862676328975	32.097401917636645	22.67355392121944
32	21.379994710053314	23.807919186463185	32.65860703439023	22.153479069093272
33	19.696751018810282	23.9640779170742	32.426382091486346	23.91278897262917
34	20.317483521568303	24.4918337222332	33.00251551211825	22.188167244080244
35	20.127565763514607	23.883427910229482	33.140586837200345	22.848419489055573
36	20.382090247481546	26.23757807162241	30.911066333082047	22.469265347813995
37	20.33135879156309	26.086622567259443	31.60482983282158	21.977188808355884
38	19.971840635090928	26.185855524990043	31.65004834664389	22.19225549327514
39	20.751829026940943	25.479641448156976	31.188757562486717	22.579771962415364
40	21.81514547663101	24.941169474653783	32.583903571828984	20.659781476886216
41	20.094116451920023	26.073676444808946	31.33624424935099	22.495962853920044
42	21.52283565919611	26.630545539689155	29.769267887483945	22.07735091363078
43	21.01143285081669	25.195446185942245	31.075339618913233	22.717781344327832
44	22.02265509485667	25.36554212971767	30.411123011082253	22.200679764343406
45	21.39256917348609	23.966555643858985	31.45127271533459	23.189602467320334
46	22.02965467302369	24.517230421777235	30.086293029597066	23.36682187560201
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4206.0
1	3604.5
2	3003.0
3	33277.0
4	63551.0
5	42807.0
6	22063.0
7	22063.0
8	20328.0
9	18593.0
10	17893.0
11	17193.0
12	16392.5
13	15592.0
14	14680.0
15	13768.0
16	12867.5
17	11967.0
18	11239.0
19	10511.0
20	10511.0
21	10475.0
22	10439.0
23	10995.0
24	11551.0
25	12703.5
26	13856.0
27	16503.5
28	19151.0
29	21788.5
30	24426.0
31	24426.0
32	28614.0
33	32802.0
34	37363.0
35	41924.0
36	45842.0
37	49760.0
38	54981.5
39	60203.0
40	66691.0
41	73179.0
42	81985.5
43	90792.0
44	90792.0
45	101610.0
46	112428.0
47	127180.0
48	141932.0
49	144295.5
50	146659.0
51	136495.0
52	126331.0
53	112638.5
54	98946.0
55	91095.5
56	83245.0
57	83245.0
58	77866.0
59	72487.0
60	66868.5
61	61250.0
62	55404.5
63	49559.0
64	42663.5
65	35768.0
66	31085.5
67	26403.0
68	22482.0
69	18561.0
70	18561.0
71	15602.0
72	12643.0
73	10216.5
74	7790.0
75	6409.5
76	5029.0
77	4167.0
78	3305.0
79	2598.5
80	1892.0
81	1892.0
82	1417.0
83	942.0
84	673.0
85	404.0
86	276.5
87	149.0
88	105.0
89	61.0
90	51.5
91	42.0
92	31.0
93	20.0
94	20.0
95	11.5
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1614383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.8477107748325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61755037827743	25.654031035338726
2	8.620353245889682	6.007991533793041
3	3.668824439993773	3.8355039890561944
4	2.157057633897787	3.006740822028584
5	1.6364616224799455	2.8513470657147133
6	1.2423154810014163	2.5975110343820558
7	1.0360821751856024	2.5273564385879044
8	0.8529510222253095	2.377871242208425
9	0.7316658142303375	2.294719081031801
>10	6.305795645393143	39.316571308570296
>50	0.08217008413459556	1.9394463439340086
>100	0.04234509675787579	2.657974643905787
>500	0.003591760297845335	0.866139198300336
>1k	0.0026465602194649836	1.8532928252040881
>5k	0.0	0.0
>10k+	1.8904001567607027E-4	2.213503437944143
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33601	2.0813524423882064	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3852	0.2386050893747023	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC	3174	0.19660762037261295	No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC	3170	0.19635984769413453	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC	2929	0.18143154381581073	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	2360	0.14618588030225788	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	2008	0.1243818845961584	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGT	1868	0.11570984084941428	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT	1685	0.10437424080902734	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.19431696196008E-5	0.0	0.0	0.036980072262901675	0.0
2	6.19431696196008E-5	0.0	0.0	0.28190336493880325	0.0
3	6.19431696196008E-5	0.0	0.0	0.6410498623932487	0.0
4	6.19431696196008E-5	0.0	0.0	1.0133902549766691	0.0
5	6.19431696196008E-5	0.0	0.0	1.7909009200418984	0.0
6	6.19431696196008E-5	0.0	0.0	3.0094469527986853	0.0
7	6.19431696196008E-5	0.0	0.0	3.9250289429460046	0.0
8	6.19431696196008E-5	0.0	0.0	5.360004410353677	0.0
9	6.19431696196008E-5	0.0	0.0	6.141789154122659	0.0
10	6.19431696196008E-5	0.0	0.0	7.2122910114886	0.0
11	6.19431696196008E-5	0.0	0.0	8.021702408907924	0.0
12	6.19431696196008E-5	0.0	0.0	8.762852433406447	0.0
13	1.238863392392016E-4	0.0	0.0	9.200604813108166	0.0
14	1.238863392392016E-4	0.0	0.0	9.413131828073016	0.0
15	1.238863392392016E-4	0.0	0.0	9.631853159999826	0.0
16	1.238863392392016E-4	0.0	0.0	10.025068400745052	0.0
17	1.238863392392016E-4	0.0	0.0	10.468457608882154	0.0
18	1.238863392392016E-4	0.0	0.0	11.047626244825423	0.0
19	1.238863392392016E-4	0.0	0.0	11.323273349632647	0.0
20	1.238863392392016E-4	0.0	0.0	11.632307822864835	0.0
21	1.238863392392016E-4	0.0	0.0	11.960234962831	0.0
22	1.238863392392016E-4	0.0	0.0	12.294790021946465	0.0
23	1.238863392392016E-4	0.0	0.0	12.63851267016563	0.0
24	1.238863392392016E-4	0.0	0.0	12.915770297382963	0.0
25	1.238863392392016E-4	0.0	0.0	13.176984643668819	0.0
26	1.858295088588024E-4	0.0	0.0	13.427173105762387	0.0
27	1.858295088588024E-4	0.0	0.0	13.70597931222021	0.0
28	1.858295088588024E-4	0.0	0.0	13.971839396227537	0.0
29	1.858295088588024E-4	0.0	0.0	14.256839919647321	0.0
30	1.858295088588024E-4	0.0	0.0	14.635684345040799	0.0
31	1.858295088588024E-4	0.0	0.0	14.935117626981949	0.0
32	2.477726784784032E-4	0.0	0.0	15.247249258695117	0.0
33	2.477726784784032E-4	0.0	0.0	15.543585382155287	0.0
34	2.477726784784032E-4	0.0	0.0	15.83180695039529	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAC	20	0.0012559193	40.000004	29
CGCGTAG	30	4.86874E-6	40.000004	1
CGTTTTT	17650	0.0	39.218132	1
ACACGAC	455	0.0	38.24176	26
CGTTATT	905	0.0	36.68508	1
CTATCGG	50	3.426976E-9	36.000004	2
CGGTCTA	50	3.426976E-9	36.000004	31
GACACGA	480	0.0	35.833336	25
CTATACG	45	5.444781E-8	35.555557	1
CACGACC	490	0.0	35.510204	27
CGAGACA	525	0.0	34.666668	22
GTTTTTT	20110	0.0	34.55992	2
CGGGTAT	220	0.0	34.545452	6
ACGGGCT	505	0.0	34.455444	5
GTACGGG	560	0.0	34.285713	3
ACGACCA	510	0.0	34.117645	28
ACTACGG	135	0.0	34.074074	2
GCGAGAC	535	0.0	34.018692	21
TCGAACG	65	3.45608E-11	33.846153	1
ACGGGAC	625	0.0	33.6	5
>>END_MODULE