##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553808_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1022724
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.647972473511917	31.0	31.0	34.0	30.0	34.0
2	31.8067337815481	31.0	31.0	34.0	30.0	34.0
3	31.74463296060325	31.0	31.0	34.0	30.0	34.0
4	35.54039212925481	37.0	35.0	37.0	33.0	37.0
5	33.64550162116074	37.0	35.0	37.0	30.0	37.0
6	34.47140675294605	37.0	35.0	37.0	30.0	37.0
7	35.42384651186439	37.0	35.0	37.0	32.0	37.0
8	35.51554280529253	37.0	35.0	37.0	33.0	37.0
9	37.23469479546779	39.0	37.0	39.0	34.0	39.0
10	36.64635620167318	38.0	35.0	39.0	32.0	39.0
11	36.402994356248605	38.0	35.0	39.0	32.0	39.0
12	36.630275616881974	39.0	35.0	39.0	32.0	39.0
13	33.160338468638656	38.0	35.0	39.0	11.0	39.0
14	35.39822376320493	39.0	35.0	41.0	18.0	41.0
15	36.742972688623716	39.0	35.0	41.0	30.0	41.0
16	37.34608848526093	39.0	36.0	41.0	32.0	41.0
17	37.51983624125375	39.0	36.0	41.0	32.0	41.0
18	37.610595820573295	39.0	36.0	40.0	33.0	41.0
19	37.60332113062762	39.0	36.0	41.0	32.0	41.0
20	37.617709176669365	39.0	36.0	41.0	32.0	41.0
21	37.53177592390518	39.0	36.0	41.0	32.0	41.0
22	37.52210175961452	39.0	36.0	41.0	32.0	41.0
23	37.399693367907666	39.0	36.0	41.0	32.0	41.0
24	37.380174905448584	39.0	36.0	41.0	32.0	41.0
25	37.16274967635452	39.0	35.0	40.0	31.0	41.0
26	37.13992142552634	39.0	35.0	40.0	32.0	41.0
27	36.74540345195771	39.0	35.0	40.0	31.0	41.0
28	36.73426066074523	39.0	35.0	40.0	30.0	41.0
29	36.7923095576128	39.0	35.0	40.0	31.0	41.0
30	36.367411931273736	39.0	35.0	40.0	30.0	41.0
31	36.41237909739089	39.0	35.0	40.0	30.0	41.0
32	36.351588502860984	39.0	35.0	40.0	30.0	41.0
33	36.31248606662208	39.0	35.0	40.0	30.0	41.0
34	36.26636903015867	39.0	35.0	40.0	30.0	41.0
35	36.20099362095736	38.0	35.0	40.0	30.0	41.0
36	36.062271932603515	38.0	35.0	40.0	29.0	41.0
37	36.0025187636156	38.0	35.0	40.0	29.0	41.0
38	35.87509728920021	38.0	35.0	40.0	29.0	41.0
39	35.86278800536606	38.0	35.0	40.0	29.0	41.0
40	35.77822462365213	38.0	35.0	40.0	29.0	41.0
41	35.624962355435095	38.0	34.0	40.0	28.0	41.0
42	35.54343889456002	38.0	34.0	40.0	28.0	41.0
43	35.44800063360203	38.0	34.0	40.0	27.0	41.0
44	35.352087171123394	38.0	34.0	40.0	27.0	41.0
45	35.29038724035028	38.0	34.0	40.0	27.0	41.0
46	34.14855034202776	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	32.0
11	18.0
12	11.0
13	13.0
14	14.0
15	27.0
16	67.0
17	154.0
18	281.0
19	516.0
20	869.0
21	1484.0
22	2281.0
23	3431.0
24	4827.0
25	6543.0
26	8989.0
27	11960.0
28	15296.0
29	19284.0
30	24248.0
31	31288.0
32	40589.0
33	54213.0
34	75419.0
35	94610.0
36	117001.0
37	154263.0
38	190818.0
39	164163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.436000328534384	24.523038473723116	22.212444413155456	19.828516784587045
2	26.536289360570397	26.781614590055575	23.583195466225494	23.09890058314853
3	28.427806524536436	26.447115741881483	23.18543419338942	21.939643540192662
4	25.923122953993456	27.712364235121107	21.701749445598224	24.662763365287212
5	20.586492543442805	35.70083424266958	19.732400921460727	23.98027229242689
6	20.074722016888234	39.99436798197754	21.656380411528428	18.274529589605798
7	83.11763486532045	5.661742562020643	7.118147222515557	4.102475350143343
8	82.43328600873745	5.223501159648156	7.368850246987457	4.974362584626937
9	74.9715465756157	7.82029169160008	10.754123302083455	6.454038430700757
10	34.0852468505677	28.246917056801244	18.119355759716207	19.548480332914846
11	27.546043702895407	28.366304105506472	23.70023584075469	20.387416350843434
12	22.87772654205827	24.785474869075134	30.611093511054793	21.725705077811806
13	21.60758914428526	29.236920224811385	29.88078895185798	19.27470167904537
14	22.016203785185446	27.513776932975077	28.34195736093022	22.12806192090926
15	20.943773686742464	26.459826893668282	31.224748808085074	21.37165061150418
16	23.169789698882592	25.88381616154505	28.997950571219604	21.94844356835275
17	22.781806235113287	25.533086150320127	27.434967791896938	24.250139822669656
18	23.13302513679155	25.520961667077337	27.809555657244772	23.536457538886346
19	24.902906355967005	26.84360589953888	26.221932799073844	22.03155494542027
20	26.623605195536626	25.079884700075482	25.477254860548886	22.819255243839002
21	26.80449466327181	26.47674250335379	25.868171667038226	20.850591166336176
22	24.705492390908983	24.65122555058843	26.145665888353065	24.497616170149524
23	23.174580825325307	26.795107966567716	26.157008146870513	23.873303061236463
24	22.64403690536254	26.265248493239625	27.288789546348767	23.801925055049065
25	24.510425100026985	26.581756172730863	25.5404195071202	23.36739922012195
26	22.816028566846967	26.733507769447083	26.09130126994184	24.359162393764105
27	24.178761816482258	25.78036694161866	26.173630422284017	23.86724081961507
28	22.83450862598316	26.500795913658038	27.375812047042995	23.288883413315812
29	24.885110743465493	26.335550940429677	24.643403303335017	24.135935012769817
30	25.487326003887656	25.117235930710535	25.050746829056518	24.34469123634529
31	25.016133384960167	26.670636457147772	24.425162604964783	23.888067552927282
32	25.01554671641616	26.3282175836296	25.601921926150162	23.054313773804076
33	23.86391636453237	25.1823561390952	24.738150273191987	26.215577223180446
34	23.14319405822099	26.18839491397484	26.191328256694867	24.477082771109313
35	24.20760635322922	25.56955737813917	24.65953668829518	25.563299580336434
36	24.167517336055475	27.25065609098838	25.037937899179052	23.54388867377709
37	23.20518536770429	27.310007392023657	26.532671571215694	22.952135669056364
38	22.31882697580188	27.155029118315397	26.562298332688002	23.96384557319472
39	22.694881512509728	24.785963759528475	26.61196960274717	25.90718512521462
40	23.65007568024218	25.536703939674833	28.203014694091465	22.610205685991527
41	21.798256421087213	26.513702621626166	27.6171283748108	24.07091258247582
42	23.62856450029529	26.218217231628476	27.558950410863538	22.5942678572127
43	23.03651816130256	24.265784317176482	28.83935450815665	23.85834301336431
44	23.775427192478126	24.36864686855887	27.160798025664796	24.69512791329821
45	23.743453756829798	23.345301371631056	27.13948240189924	25.771762469639903
46	24.173481799586202	24.886968527188174	26.275808527031728	24.66374114619389
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3916.0
1	3375.0
2	2834.0
3	5180.0
4	7526.0
5	5212.5
6	2899.0
7	2899.0
8	2735.0
9	2571.0
10	2606.5
11	2642.0
12	2661.0
13	2680.0
14	2697.5
15	2715.0
16	2635.0
17	2555.0
18	2736.0
19	2917.0
20	2917.0
21	3007.0
22	3097.0
23	3768.0
24	4439.0
25	5628.5
26	6818.0
27	7993.0
28	9168.0
29	11096.5
30	13025.0
31	13025.0
32	14841.5
33	16658.0
34	18987.5
35	21317.0
36	24093.0
37	26869.0
38	29690.5
39	32512.0
40	36647.5
41	40783.0
42	47780.5
43	54778.0
44	54778.0
45	64911.0
46	75044.0
47	82857.5
48	90671.0
49	96640.0
50	102609.0
51	97433.0
52	92257.0
53	82957.5
54	73658.0
55	69075.5
56	64493.0
57	64493.0
58	61831.0
59	59169.0
60	55707.0
61	52245.0
62	47398.0
63	42551.0
64	37456.5
65	32362.0
66	28527.0
67	24692.0
68	21772.5
69	18853.0
70	18853.0
71	15513.0
72	12173.0
73	9876.0
74	7579.0
75	6675.0
76	5771.0
77	4420.0
78	3069.0
79	2248.0
80	1427.0
81	1427.0
82	1086.5
83	746.0
84	548.5
85	351.0
86	254.0
87	157.0
88	105.5
89	54.0
90	50.0
91	46.0
92	35.0
93	24.0
94	24.0
95	13.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1022724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.88758407131165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77744883975167	27.553571500174172
2	9.080774473135833	6.517741146745659
3	3.4509379265035287	3.7153747488681983
4	1.8421374337268375	2.644394480951287
5	1.18962146657938	2.1346320197452284
6	0.7788434508953916	1.6770485893439313
7	0.5889568201094658	1.479536617723558
8	0.46933152907962794	1.3474539765729918
9	0.38879514892583117	1.2557626734234508
>10	5.1706534125474155	42.18815682972301
>50	0.21777412228885085	4.7738722122239485
>100	0.03940092687917584	2.575111611685977
>500	0.0034608922250596195	0.7353776600922839
>1k	0.00186355735195518	1.4019659327262795
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3754	0.3670589523664254	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3486	0.34085442406749034	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	1987	0.19428506615665614	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCT	1571	0.1536093804389063	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC	1517	0.14832936354285223	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCT	1258	0.12300483805992622	No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTT	1101	0.10765367782510236	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.777809066766791E-5	0.0	0.0	0.1294581920439923	0.0
2	9.777809066766791E-5	0.0	0.0	0.5625173556110935	0.0
3	9.777809066766791E-5	0.0	0.0	0.8241715262377729	0.0
4	9.777809066766791E-5	0.0	0.0	1.1357903011956305	0.0
5	9.777809066766791E-5	0.0	0.0	1.9207528130756686	0.0
6	9.777809066766791E-5	0.0	0.0	3.0413875102178105	0.0
7	9.777809066766791E-5	0.0	0.0	3.8478612020447356	0.0
8	9.777809066766791E-5	0.0	0.0	5.204141097695957	0.0
9	9.777809066766791E-5	0.0	0.0	5.789147414160614	0.0
10	9.777809066766791E-5	0.0	0.0	6.786679495152162	0.0
11	9.777809066766791E-5	0.0	0.0	7.795162722298489	0.0
12	9.777809066766791E-5	0.0	0.0	8.719556791470621	0.0
13	9.777809066766791E-5	0.0	0.0	9.129735881821489	0.0
14	9.777809066766791E-5	0.0	0.0	9.338296549215624	0.0
15	9.777809066766791E-5	0.0	0.0	9.604350733922349	0.0
16	9.777809066766791E-5	0.0	0.0	10.187988157117658	0.0
17	9.777809066766791E-5	0.0	0.0	10.807021249134664	0.0
18	9.777809066766791E-5	0.0	0.0	11.620241629217658	0.0
19	9.777809066766791E-5	0.0	0.0	11.977327216335981	0.0
20	9.777809066766791E-5	0.0	0.0	12.363844008745273	0.0
21	9.777809066766791E-5	0.0	0.0	12.802867635843102	0.0
22	1.9555618133533582E-4	0.0	0.0	13.241011260124921	0.0
23	1.9555618133533582E-4	0.0	0.0	13.694799378913569	0.0
24	1.9555618133533582E-4	0.0	0.0	14.070462803258748	0.0
25	1.9555618133533582E-4	0.0	0.0	14.379734904040582	0.0
26	1.9555618133533582E-4	0.0	0.0	14.68802922391574	0.0
27	1.9555618133533582E-4	0.0	0.0	15.025265858628526	0.0
28	1.9555618133533582E-4	0.0	0.0	15.356146917447914	0.0
29	1.9555618133533582E-4	0.0	0.0	15.7030635831368	0.0
30	1.9555618133533582E-4	0.0	0.0	16.129473836538498	0.0
31	1.9555618133533582E-4	0.0	0.0	16.487439426472832	0.0
32	1.9555618133533582E-4	0.0	0.0	16.8489250276712	0.0
33	1.9555618133533582E-4	0.0	0.0	17.201708378800145	0.0
34	1.9555618133533582E-4	0.0	0.0	17.54158502196096	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCCC	20	0.0012557037	40.0	19
GTTAACG	45	1.2205419E-9	40.0	1
TATTACG	40	1.9279469E-8	40.0	1
ACGCATT	285	0.0	38.596493	17
TACGCAT	295	0.0	37.966103	16
CTACGCA	295	0.0	37.966103	15
GCGATTA	180	0.0	36.666668	9
ACACGAC	390	0.0	36.410255	26
CGCATTT	300	0.0	36.0	18
CATAGCG	50	3.423338E-9	36.0	1
ACGCTAG	50	3.423338E-9	36.0	1
TTAACGG	145	0.0	35.86207	2
CGTACGG	90	0.0	35.555557	2
CACGACC	400	0.0	35.5	27
GCACTTA	1090	0.0	35.045868	36
AACGAGT	40	8.69868E-7	35.0	33
TCGTAAG	40	8.69868E-7	35.0	1
TATAGCG	40	8.69868E-7	35.0	1
ACGACCA	410	0.0	34.634148	28
CGTTTTT	1995	0.0	34.586464	1
>>END_MODULE