FastQCFastQC Report
Sat 18 Jun 2016
SRR3553807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468876
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28340.6044241974423942No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA20540.4380689137426526No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.24036205734565214No Hit
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT10620.22649911703734038No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT9890.2109299686910825No Hit
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTT8430.17979167199856677No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6490.13841612707837467No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG5560.11858145863725164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAA401.924127E-840.08
CTCGTAA257.787585E-540.033
ACGTTGG304.861342E-640.02
TCCGAGT200.001255008740.012
GCGTTAG200.001255008740.01
ACGGGAT1200.040.05
CCGTCAA200.001255008740.013
TCGGCAA200.001255008740.040
CGTTAGG353.0512092E-740.02
GTTACGG304.861342E-640.02
CTACGTC200.001255008740.014
CATAGCG401.924127E-840.01
TTGCGTC304.861342E-640.023
GTCAACG200.001255008740.01
CGAGCTA200.001255008740.04
TCACTAG200.001255008740.038
TCGTAAG401.924127E-840.01
AACTCGT200.001255008740.012
GCGTGCA257.787585E-540.032
CGCACAT200.001255008740.022