##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553807_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468876
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.737947772971957	31.0	31.0	34.0	30.0	34.0
2	31.895136453987835	31.0	31.0	34.0	30.0	34.0
3	31.851041640007168	31.0	31.0	34.0	30.0	34.0
4	35.62824499441217	37.0	35.0	37.0	33.0	37.0
5	33.733010433462155	37.0	35.0	37.0	30.0	37.0
6	34.556983082947305	37.0	35.0	37.0	30.0	37.0
7	35.484236770489424	37.0	35.0	37.0	32.0	37.0
8	35.568182205956376	37.0	35.0	37.0	35.0	37.0
9	37.32964152569123	39.0	37.0	39.0	34.0	39.0
10	36.751226337027276	39.0	37.0	39.0	32.0	39.0
11	36.519685375237806	39.0	35.0	39.0	32.0	39.0
12	36.75318207799077	39.0	35.0	39.0	32.0	39.0
13	33.15499407092707	38.0	35.0	39.0	10.0	39.0
14	35.42279408628294	39.0	35.0	41.0	18.0	41.0
15	36.8297886861345	39.0	35.0	41.0	30.0	41.0
16	37.45852421535758	39.0	36.0	41.0	32.0	41.0
17	37.61588778269734	39.0	36.0	41.0	33.0	41.0
18	37.73960066200872	39.0	37.0	41.0	33.0	41.0
19	37.74045163326765	39.0	37.0	41.0	33.0	41.0
20	37.737184244874975	39.0	36.0	41.0	33.0	41.0
21	37.64103728917667	39.0	36.0	41.0	33.0	41.0
22	37.639804553869254	39.0	36.0	41.0	33.0	41.0
23	37.535237461503684	39.0	36.0	41.0	33.0	41.0
24	37.54773116986154	39.0	36.0	41.0	33.0	41.0
25	37.362968034192406	39.0	36.0	41.0	32.0	41.0
26	37.31449039831427	39.0	36.0	41.0	32.0	41.0
27	36.94033817043312	39.0	35.0	40.0	31.0	41.0
28	36.98179689299516	39.0	35.0	40.0	31.0	41.0
29	37.01386933858846	39.0	35.0	40.0	31.0	41.0
30	36.56124647028212	39.0	35.0	40.0	31.0	41.0
31	36.64846142690178	39.0	35.0	40.0	30.0	41.0
32	36.59032238800877	39.0	35.0	40.0	30.0	41.0
33	36.57973749989336	39.0	35.0	40.0	30.0	41.0
34	36.572748445217925	39.0	35.0	40.0	30.0	41.0
35	36.463915832757486	39.0	35.0	40.0	30.0	41.0
36	36.344984601472454	39.0	35.0	40.0	30.0	41.0
37	36.326453049420316	38.0	35.0	40.0	30.0	41.0
38	36.16247366041341	38.0	35.0	40.0	30.0	41.0
39	36.17506974125355	38.0	35.0	40.0	30.0	41.0
40	36.03482583881453	38.0	35.0	40.0	30.0	41.0
41	35.85096699340551	38.0	35.0	40.0	29.0	41.0
42	35.780485672118004	38.0	35.0	40.0	29.0	41.0
43	35.7156113769952	38.0	35.0	40.0	29.0	41.0
44	35.691201938252334	38.0	35.0	40.0	29.0	41.0
45	35.63816232863273	38.0	34.0	40.0	29.0	41.0
46	34.425986828073945	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	11.0
10	10.0
11	19.0
12	4.0
13	8.0
14	9.0
15	17.0
16	23.0
17	50.0
18	113.0
19	212.0
20	351.0
21	598.0
22	989.0
23	1297.0
24	1906.0
25	2706.0
26	3476.0
27	4541.0
28	5880.0
29	7685.0
30	9945.0
31	13212.0
32	17364.0
33	24287.0
34	34288.0
35	43791.0
36	54185.0
37	73619.0
38	90727.0
39	77547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.16452111005895	24.332019553144114	22.596166150538735	17.9072931862582
2	25.29965278666428	27.32300224366357	24.004001057848985	23.37334391182317
3	27.947900937561315	27.863870191692474	23.029542992177035	21.158685878569177
4	24.862223700935854	29.185541593086445	21.626186880966397	24.326047825011305
5	20.656634163403545	35.7198918264104	19.164128682210222	24.45934532797584
6	19.827417056961753	40.72590620974415	21.22117574795895	18.22550098533514
7	82.23197604483914	6.18223154949283	6.931896706165383	4.65389569950264
8	81.59150820259514	5.713237615062405	7.011022103925131	5.684232078417321
9	74.32135575290695	8.675854596951007	10.385261774968221	6.6175278751738205
10	33.108327148329195	30.44706916114282	17.465811856439657	18.97879183408833
11	27.77386771769082	29.373864305274743	22.73906107371672	20.11320690331772
12	23.227036572569293	25.107704382395347	29.75008317764185	21.91517586739351
13	21.854392206041684	31.20718484204779	28.111696909204138	18.826726042706387
14	20.71464523669371	29.033689077709248	27.9009802165178	22.350685469079245
15	19.615420708246955	28.003992526808798	31.109291155870633	21.271295609073615
16	21.988329537020448	27.013538760781103	29.082955834804935	21.91517586739351
17	21.206459703631666	26.944010783234802	26.807727416203857	25.04180209692968
18	23.321304566665813	26.637959716428224	26.433001475869954	23.60773424103601
19	25.586082461034476	28.030865303406443	24.827033160153217	21.556019075405864
20	26.664192665011644	25.965927025482216	24.952439450942254	22.417440858563882
21	26.29586500482004	28.16885487847533	25.4935206749759	20.04175944172873
22	23.528182291266774	26.181335790272907	25.073580221636426	25.216901696823896
23	22.857855808358714	28.265255632619287	25.61018264957046	23.26670590945154
24	22.845699076088348	26.299064144891187	27.23918477379947	23.616052005220997
25	23.134261510505976	27.854272771479028	24.63081923578942	24.380646482225576
26	21.293049761557427	28.480024569395745	26.385654202816948	23.841271466229877
27	22.919919125739003	27.730359412723192	25.339322123546527	24.01039933799128
28	21.599953932382977	27.972001126097307	26.33276175364062	24.095283187879097
29	23.0796628532917	28.66728090156033	25.00277258806166	23.25028365708631
30	24.472568440269924	27.895435040394474	25.758196196862283	21.87380032247332
31	25.13734974705466	28.49538044173726	23.85001578242435	22.51725402878373
32	23.735060015867735	29.33312858836878	25.211783072710052	21.72002832305343
33	23.702642063146758	28.393221235465237	23.591951816685008	24.312184884702994
34	24.104240780078314	27.516443579965706	26.645850928603725	21.733464711352255
35	23.994190361630793	27.371202620735545	25.674805279007668	22.95980173862599
36	25.290481918460316	28.407510727783038	24.499014664858084	21.802992688898556
37	26.259394808008942	28.728491114921646	24.41114495090386	20.600969126165552
38	24.920448050230764	28.103592421023894	25.341454883593954	21.634504645151384
39	27.85597897951697	24.468302920175056	24.178674105733712	23.497043994574256
40	26.354942458133916	24.76369018674447	28.58687584777212	20.29449150734949
41	23.24836417304362	26.320818297375	27.12273607520965	23.30808145437173
42	24.152441157150292	27.20698009708324	26.88280056987348	21.757778175892987
43	25.06035710934234	25.509516375331643	26.63134816028118	22.79877835504483
44	24.526313993465223	25.84052073469318	25.962514609406323	23.67065066243527
45	24.475554304336328	24.125141828543153	26.63369419633336	24.765609670787157
46	25.444040641875464	25.731536696269373	25.84180039072164	22.98262227113352
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3167.0
1	2620.0
2	2073.0
3	2365.5
4	2658.0
5	1849.0
6	1040.0
7	1040.0
8	989.0
9	938.0
10	1030.0
11	1122.0
12	1153.5
13	1185.0
14	1252.0
15	1319.0
16	1253.5
17	1188.0
18	1285.5
19	1383.0
20	1383.0
21	1364.0
22	1345.0
23	1566.0
24	1787.0
25	2423.5
26	3060.0
27	3376.5
28	3693.0
29	4723.5
30	5754.0
31	5754.0
32	6319.5
33	6885.0
34	8307.0
35	9729.0
36	11165.0
37	12601.0
38	14479.0
39	16357.0
40	18521.5
41	20686.0
42	24697.0
43	28708.0
44	28708.0
45	32395.5
46	36083.0
47	40779.0
48	45475.0
49	46351.5
50	47228.0
51	44460.5
52	41693.0
53	37240.5
54	32788.0
55	31566.5
56	30345.0
57	30345.0
58	28284.5
59	26224.0
60	25208.5
61	24193.0
62	21423.0
63	18653.0
64	16229.5
65	13806.0
66	11490.0
67	9174.0
68	7723.5
69	6273.0
70	6273.0
71	5207.0
72	4141.0
73	3322.0
74	2503.0
75	2055.0
76	1607.0
77	1310.5
78	1014.0
79	792.0
80	570.0
81	570.0
82	438.0
83	306.0
84	184.5
85	63.0
86	46.5
87	30.0
88	22.5
89	15.0
90	11.5
91	8.0
92	6.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	468876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.0000931526904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.92597467524268	28.092681356927912
2	9.485767560485769	7.019485667254843
3	3.546202646700456	3.936294847987023
4	1.9290355151695755	2.8549797502448957
5	1.2095331068953403	2.2376418813195307
6	0.8420403626667421	1.8693343114196797
7	0.6184635684555498	1.6018246751080458
8	0.5312358256066637	1.5724620026794356
9	0.4447450686719526	1.481004807305574
>10	5.30117571669355	41.565675237139
>50	0.11992257530471712	2.777568760859749
>100	0.041313040296361854	2.8438075022331515
>500	0.002295168905353436	0.6447662100994461
>1k	0.002295168905353436	1.5024729894217257
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2834	0.6044241974423942	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	2054	0.4380689137426526	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1127	0.24036205734565214	No Hit
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	1062	0.22649911703734038	No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	989	0.2109299686910825	No Hit
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTT	843	0.17979167199856677	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	649	0.13841612707837467	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	556	0.11858145863725164	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20901048464839317	0.0
2	0.0	0.0	0.0	0.8754979994710755	0.0
3	0.0	0.0	0.0	1.1749375101306103	0.0
4	0.0	0.0	0.0	1.555421902592583	0.0
5	0.0	0.0	0.0	2.586398109521494	0.0
6	0.0	0.0	0.0	3.6470196811097177	0.0
7	0.0	0.0	0.0	4.38751396957831	0.0
8	0.0	0.0	0.0	5.827766829609534	0.0
9	0.0	0.0	0.0	6.430058267004496	0.0
10	0.0	0.0	0.0	7.61374009332958	0.0
11	0.0	0.0	0.0	8.791876743531338	0.0
12	0.0	0.0	0.0	9.816454670317952	0.0
13	0.0	0.0	0.0	10.26987945640212	0.0
14	0.0	0.0	0.0	10.481449253107431	0.0
15	0.0	0.0	0.0	10.809681024407306	0.0
16	0.0	0.0	0.0	11.539724788643479	0.0
17	0.0	0.0	0.0	12.359344474871822	0.0
18	0.0	0.0	0.0	13.430843122701951	0.0
19	0.0	0.0	0.0	13.875523592591644	0.0
20	0.0	0.0	0.0	14.358807019339869	0.0
21	0.0	0.0	0.0	14.895835999283392	0.0
22	0.0	0.0	0.0	15.419855142937578	0.0
23	0.0	0.0	0.0	15.953898258814696	0.0
24	0.0	0.0	0.0	16.35784301179843	0.0
25	0.0	0.0	0.0	16.717639631800306	0.0
26	0.0	0.0	0.0	17.039686398962626	0.0
27	0.0	0.0	0.0	17.429128383623816	0.0
28	0.0	0.0	0.0	17.80790656804784	0.0
29	0.0	0.0	0.0	18.222728397273478	0.0
30	0.0	0.0	0.0	18.704732167993242	0.0
31	0.0	0.0	0.0	19.088415700526365	0.0
32	0.0	0.0	0.0	19.487241829396257	0.0
33	0.0	0.0	0.0	19.874551054010016	0.0
34	0.0	0.0	0.0	20.262500106638	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACAA	40	1.924127E-8	40.0	8
CTCGTAA	25	7.787585E-5	40.0	33
ACGTTGG	30	4.861342E-6	40.0	2
TCCGAGT	20	0.0012550087	40.0	12
GCGTTAG	20	0.0012550087	40.0	1
ACGGGAT	120	0.0	40.0	5
CCGTCAA	20	0.0012550087	40.0	13
TCGGCAA	20	0.0012550087	40.0	40
CGTTAGG	35	3.0512092E-7	40.0	2
GTTACGG	30	4.861342E-6	40.0	2
CTACGTC	20	0.0012550087	40.0	14
CATAGCG	40	1.924127E-8	40.0	1
TTGCGTC	30	4.861342E-6	40.0	23
GTCAACG	20	0.0012550087	40.0	1
CGAGCTA	20	0.0012550087	40.0	4
TCACTAG	20	0.0012550087	40.0	38
TCGTAAG	40	1.924127E-8	40.0	1
AACTCGT	20	0.0012550087	40.0	12
GCGTGCA	25	7.787585E-5	40.0	32
CGCACAT	20	0.0012550087	40.0	22
>>END_MODULE