FastQCFastQC Report
Sat 18 Jun 2016
SRR3553806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957914
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43130.4502491872965631No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35070.36610802222328936No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG17910.18696876755115804No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA14870.1552331420148364No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT13700.14301910192355474No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCG12200.1273600761655013No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTA11900.1242282710138906No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGA10750.1122230179327163No Hit
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT10500.10961318030637404No Hit
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT10120.10564622711433384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAACG304.8666625E-640.0000041
TTTAGCG800.040.01
CAATACG200.00125566440.01
TACGTTA200.00125566440.030
ACGCATT2900.037.93103817
TACGCAT2900.037.93103816
CTACGCA2900.037.93103815
CGCATTT3000.036.66666818
TGTTACG503.423338E-936.01
TACTCGA503.423338E-936.032
CGACGGT455.440961E-835.55555728
TCGACAC455.440961E-835.55555734
TTCACGG850.035.294122
CGTTCGG408.697916E-735.02
TCTACGC3200.035.014
CGTTTTT19350.034.93541
TCACCGC3100.034.8387124
GCTACGA1850.034.59459310
AACGGGA5500.034.5454564
CGGGCTA1100.034.5454526