##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553806_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	957914
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.685324569846564	31.0	31.0	34.0	30.0	34.0
2	31.850427073829174	31.0	31.0	34.0	30.0	34.0
3	31.77993431560662	31.0	31.0	34.0	30.0	34.0
4	35.56784638286944	37.0	35.0	37.0	33.0	37.0
5	33.659402618606684	37.0	35.0	37.0	30.0	37.0
6	34.48534419582551	37.0	35.0	37.0	30.0	37.0
7	35.42372279766242	37.0	35.0	37.0	32.0	37.0
8	35.50276642788392	37.0	35.0	37.0	33.0	37.0
9	37.263996559190076	39.0	37.0	39.0	34.0	39.0
10	36.673287998713874	39.0	37.0	39.0	32.0	39.0
11	36.437792954273554	39.0	35.0	39.0	32.0	39.0
12	36.62855224999321	39.0	35.0	39.0	32.0	39.0
13	33.12145140377946	38.0	35.0	39.0	10.0	39.0
14	35.36970646634249	39.0	35.0	41.0	18.0	41.0
15	36.75128664994978	39.0	35.0	41.0	30.0	41.0
16	37.3519439114576	39.0	36.0	41.0	32.0	41.0
17	37.52023459308456	39.0	36.0	41.0	32.0	41.0
18	37.61355612299225	39.0	36.0	41.0	33.0	41.0
19	37.59935025482454	39.0	36.0	41.0	32.0	41.0
20	37.60035556427821	39.0	36.0	41.0	32.0	41.0
21	37.52321189584869	39.0	36.0	41.0	32.0	41.0
22	37.51746816520063	39.0	36.0	41.0	32.0	41.0
23	37.392982042229264	39.0	36.0	41.0	32.0	41.0
24	37.35932557619995	39.0	36.0	41.0	32.0	41.0
25	37.15548995003727	39.0	35.0	41.0	31.0	41.0
26	37.11426808669672	39.0	35.0	40.0	31.0	41.0
27	36.71456623454715	39.0	35.0	40.0	31.0	41.0
28	36.74932092024962	39.0	35.0	40.0	31.0	41.0
29	36.801830853291634	39.0	35.0	40.0	31.0	41.0
30	36.337942654559804	39.0	35.0	40.0	30.0	41.0
31	36.43404731531223	39.0	35.0	40.0	30.0	41.0
32	36.39264171940279	39.0	35.0	40.0	30.0	41.0
33	36.313512486507136	39.0	35.0	40.0	30.0	41.0
34	36.25638000906136	39.0	35.0	40.0	30.0	41.0
35	36.17476516681038	38.0	35.0	40.0	30.0	41.0
36	36.061309261582984	38.0	35.0	40.0	29.0	41.0
37	35.987766125142755	38.0	35.0	40.0	29.0	41.0
38	35.867562223748685	38.0	35.0	40.0	29.0	41.0
39	35.80606505385661	38.0	35.0	40.0	29.0	41.0
40	35.689823929914375	38.0	35.0	40.0	28.0	41.0
41	35.57086126729539	38.0	34.0	40.0	28.0	41.0
42	35.49764592646104	38.0	34.0	40.0	28.0	41.0
43	35.39416899638172	38.0	34.0	40.0	27.0	41.0
44	35.31010925824239	38.0	34.0	40.0	27.0	41.0
45	35.245065840983635	38.0	34.0	40.0	27.0	41.0
46	34.158501702658064	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	15.0
10	28.0
11	24.0
12	19.0
13	11.0
14	17.0
15	32.0
16	70.0
17	139.0
18	275.0
19	521.0
20	891.0
21	1418.0
22	2231.0
23	3309.0
24	4675.0
25	6254.0
26	8561.0
27	11353.0
28	14650.0
29	18445.0
30	22804.0
31	29481.0
32	38150.0
33	50571.0
34	70602.0
35	88494.0
36	106536.0
37	142216.0
38	175203.0
39	160915.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.059126393392305	23.92187607655802	22.7400372058452	20.27896032420447
2	26.127815231847535	27.29681370143875	23.16168257275705	23.41368849395666
3	28.163697367404588	26.551757255870566	23.801510365231117	21.483035011493726
4	25.264689732063626	28.125593738059994	22.524360224404276	24.0853563054721
5	20.869096808272978	34.718461156220705	19.808563190432544	24.603878845073776
6	20.284284392962206	40.13763239706278	21.645784485872426	17.93229872410258
7	82.67005180005721	5.580146025634869	7.702361589871325	4.047440584436599
8	82.07573957578656	5.767741154216349	7.514557674279737	4.641961595717361
9	75.4408015750892	7.530529880552951	10.255409149464358	6.773259394893487
10	34.53483298083127	27.911795839710035	17.856926613453815	19.69644456600488
11	28.437103957140202	27.467601475706587	23.590009123992342	20.50528544316087
12	24.225556782759202	24.221589829567165	30.29363805101502	21.259215336658617
13	21.84277502990874	30.159701184031135	28.90729230390202	19.090231482158107
14	21.80853396025113	27.766793261190465	28.10826441622108	22.316408362337327
15	20.913150867405633	26.496115517676955	30.80099048557595	21.789743129341467
16	23.217115523940564	25.352902243833995	29.24093394605361	22.18904828617183
17	22.63272068265001	25.7929208676353	27.32291207770217	24.25144637201252
18	23.888052580920625	25.38745649400677	27.507792975152256	23.21669794992035
19	24.894823543658408	27.760112076867028	25.791250571554443	21.55381380792013
20	27.270506538165222	25.256233858154282	25.287656303175442	22.185603300505054
21	26.786955822756532	27.104312078119747	25.452702434665326	20.656029664458398
22	24.468898043039356	24.93104808991204	25.940742070791323	24.65931179625728
23	23.63166213250876	26.693628029238532	26.54612000659767	23.128589831655034
24	23.253235676689137	25.46585601630209	27.64193862914625	23.638969677862523
25	24.267001004265516	27.224573395941597	25.158417143918975	23.350008455873912
26	23.149572926170826	26.62671179249912	26.03093805915771	24.192777222172346
27	23.877300049900096	25.71932344657245	26.894063558941617	23.50931294458584
28	22.382176270521153	26.978204724014894	27.165695459091317	23.47392354637264
29	23.691062036884315	27.620642354115297	25.034397659915193	23.653897949085202
30	24.242572924082957	26.38472764778467	26.404771200754972	22.967928227377403
31	24.024494892025796	26.912958783356334	24.94702029618525	24.115526028432615
32	24.514100430727602	27.60341742578144	25.00433233045973	22.878149813031232
33	23.324954014661024	27.52762774111246	24.827072158878565	24.320346085347953
34	22.645039116246345	28.001678647561263	25.63820969314573	23.715072543046663
35	24.578928797365943	26.662205584217375	25.31845238716628	23.440413231250403
36	24.87363166213251	28.543898512810127	24.012594032449677	22.56987579260769
37	23.82416375582777	28.71917520779527	25.254981136093633	22.201679900283324
38	23.79514236142284	28.60361159770084	24.824253534242114	22.77699250663421
39	24.802957259211162	26.988226500500044	24.216578941324588	23.99223729896421
40	24.84283557814167	26.361447896157692	27.815858208565697	20.97985831713494
41	23.06939871428959	26.333992404328573	26.645920197428996	23.95068868395284
42	24.02512125305612	26.315932327954282	26.2994381541558	23.359508264833796
43	23.668304252782608	24.617032426710537	28.058990681835738	23.655672638671113
44	23.751088302290185	25.697922778036443	26.20214340744576	24.348845512227612
45	23.64544207517585	25.139104345484043	26.44538027422086	24.770073305119247
46	24.195595846808796	26.380551907582518	25.492893934110995	23.93095831149769
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4027.0
1	3478.0
2	2929.0
3	5722.0
4	8515.0
5	5687.5
6	2860.0
7	2860.0
8	2678.5
9	2497.0
10	2590.0
11	2683.0
12	2600.0
13	2517.0
14	2505.0
15	2493.0
16	2515.5
17	2538.0
18	2526.0
19	2514.0
20	2514.0
21	2780.5
22	3047.0
23	3804.0
24	4561.0
25	5573.5
26	6586.0
27	7502.0
28	8418.0
29	10839.0
30	13260.0
31	13260.0
32	14998.0
33	16736.0
34	19212.5
35	21689.0
36	24947.5
37	28206.0
38	30654.0
39	33102.0
40	37436.0
41	41770.0
42	48030.5
43	54291.0
44	54291.0
45	60396.5
46	66502.0
47	74468.5
48	82435.0
49	84018.0
50	85601.0
51	81899.0
52	78197.0
53	73350.5
54	68504.0
55	64204.5
56	59905.0
57	59905.0
58	58471.0
59	57037.0
60	53612.5
61	50188.0
62	45739.0
63	41290.0
64	36652.5
65	32015.0
66	27873.5
67	23732.0
68	20202.5
69	16673.0
70	16673.0
71	14308.0
72	11943.0
73	9919.5
74	7896.0
75	6368.5
76	4841.0
77	3881.5
78	2922.0
79	2284.5
80	1647.0
81	1647.0
82	1194.5
83	742.0
84	548.0
85	354.0
86	241.5
87	129.0
88	100.5
89	72.0
90	49.5
91	27.0
92	19.0
93	11.0
94	11.0
95	10.0
96	9.0
97	5.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	957914.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.08437247028004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.04783489847473	27.03174937606729
2	8.670127043355807	6.083719331074859
3	3.300953710949408	3.4743566850630647
4	1.8217376306908943	2.556580863131392
5	1.1680037180423757	2.0489338745235326
6	0.816694884443555	1.7191936512233996
7	0.6159436567091152	1.5126997670882232
8	0.47658242557015	1.3376476265194126
9	0.4287531680565979	1.353828226131923
>10	5.369717492142363	42.665899669367164
>50	0.24180133656551758	5.1947834281476934
>100	0.035456279652449796	2.36566775618122
>500	0.0034875029165783557	0.8180575150930516
>1k	0.0029062524304819632	1.8368822303878005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4313	0.4502491872965631	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3507	0.36610802222328936	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	1791	0.18696876755115804	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	1487	0.1552331420148364	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	1370	0.14301910192355474	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCG	1220	0.1273600761655013	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTA	1190	0.1242282710138906	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGA	1075	0.1122230179327163	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	1050	0.10961318030637404	No Hit
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT	1012	0.10564622711433384	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0439350505368959E-4	0.0	0.0	0.1221404009128168	0.0
2	1.0439350505368959E-4	0.0	0.0	0.528335529076723	0.0
3	1.0439350505368959E-4	0.0	0.0	0.750276120820867	0.0
4	1.0439350505368959E-4	0.0	0.0	1.0387153752842113	0.0
5	1.0439350505368959E-4	0.0	0.0	1.843902479763319	0.0
6	1.0439350505368959E-4	0.0	0.0	2.7614169956801966	0.0
7	1.0439350505368959E-4	0.0	0.0	3.409074301033287	0.0
8	1.0439350505368959E-4	0.0	0.0	4.556567708583443	0.0
9	1.0439350505368959E-4	0.0	0.0	5.033750420183858	0.0
10	1.0439350505368959E-4	0.0	0.0	5.960033990525245	0.0
11	1.0439350505368959E-4	0.0	0.0	6.782759203853373	0.0
12	1.0439350505368959E-4	0.0	0.0	7.581160730503991	0.0
13	1.0439350505368959E-4	0.0	0.0	7.917412210281925	0.0
14	1.0439350505368959E-4	0.0	0.0	8.076507911983748	0.0
15	1.0439350505368959E-4	0.0	0.0	8.312124052889926	0.0
16	1.0439350505368959E-4	0.0	0.0	8.799850508500763	0.0
17	1.0439350505368959E-4	0.0	0.0	9.346768081477043	0.0
18	1.0439350505368959E-4	0.0	0.0	10.044012301730636	0.0
19	1.0439350505368959E-4	0.0	0.0	10.358027964932134	0.0
20	1.0439350505368959E-4	0.0	0.0	10.69574095378082	0.0
21	2.0878701010737917E-4	0.0	0.0	11.0573600552868	0.0
22	2.0878701010737917E-4	0.0	0.0	11.4265998826617	0.0
23	2.0878701010737917E-4	0.0	0.0	11.825696252481956	0.0
24	2.0878701010737917E-4	0.0	0.0	12.139920702693562	0.0
25	2.0878701010737917E-4	0.0	0.0	12.418651361186912	0.0
26	2.0878701010737917E-4	0.0	0.0	12.671910004447163	0.0
27	2.0878701010737917E-4	0.0	0.0	12.96807437828448	0.0
28	2.0878701010737917E-4	0.0	0.0	13.242211722555469	0.0
29	2.0878701010737917E-4	0.0	0.0	13.554974663696324	0.0
30	2.0878701010737917E-4	0.0	0.0	13.947807423213357	0.0
31	2.0878701010737917E-4	0.0	0.0	14.249504652818521	0.0
32	2.0878701010737917E-4	0.0	0.0	14.554438081080347	0.0
33	2.0878701010737917E-4	0.0	0.0	14.852899112028846	0.0
34	2.0878701010737917E-4	0.0	0.0	15.148332731330788	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAACG	30	4.8666625E-6	40.000004	1
TTTAGCG	80	0.0	40.0	1
CAATACG	20	0.001255664	40.0	1
TACGTTA	20	0.001255664	40.0	30
ACGCATT	290	0.0	37.931038	17
TACGCAT	290	0.0	37.931038	16
CTACGCA	290	0.0	37.931038	15
CGCATTT	300	0.0	36.666668	18
TGTTACG	50	3.423338E-9	36.0	1
TACTCGA	50	3.423338E-9	36.0	32
CGACGGT	45	5.440961E-8	35.555557	28
TCGACAC	45	5.440961E-8	35.555557	34
TTCACGG	85	0.0	35.29412	2
CGTTCGG	40	8.697916E-7	35.0	2
TCTACGC	320	0.0	35.0	14
CGTTTTT	1935	0.0	34.9354	1
TCACCGC	310	0.0	34.83871	24
GCTACGA	185	0.0	34.594593	10
AACGGGA	550	0.0	34.545456	4
CGGGCTA	110	0.0	34.545452	6
>>END_MODULE