FastQCFastQC Report
Sat 18 Jun 2016
SRR3553804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402930
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20430.5070359615814161No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA16620.4124785942967761No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG15720.3901422083240265No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA12510.3104757650212196No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCG12200.30278212096393914No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.2873948328493783No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT8090.20077929168838257No Hit
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT5550.13774104683195593No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT5520.13699650063286425No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC4470.1109373836646564No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.10994465539920086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGA200.001254798340.0000041
CGCATCG200.001254798340.00000421
CCATGCG200.001254798340.0000041
GGTATAC200.001254798340.0000048
GCATCGC200.001254798340.00000422
CTCACGA257.7856326E-540.024
TACGCAT1350.040.016
CATAGCG257.7856326E-540.01
CGTACGG257.7856326E-540.02
TAATGGC257.7856326E-540.015
TCGCGAG353.0497904E-740.01
CTACGCA1500.037.33333215
ACGCATT1450.037.24138317
TCGTTCA700.037.14285716
TAGGGTA1300.036.9230775
TTACACG1900.036.84210634
GCACTTA4300.036.74418336
TACGCGG552.1645974E-1036.363642
CACCGCT1550.036.12903225
GTCTTAC2050.036.0975631