##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553804_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 402930 Sequences flagged as poor quality 0 Sequence length 46 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67168738986921 31.0 31.0 34.0 30.0 34.0 2 31.82775171866081 31.0 31.0 34.0 30.0 34.0 3 31.78107363561909 31.0 31.0 34.0 30.0 34.0 4 35.55764276673368 37.0 35.0 37.0 33.0 37.0 5 33.644920954011866 37.0 35.0 37.0 30.0 37.0 6 34.46666914848733 37.0 35.0 37.0 30.0 37.0 7 35.36536867445958 37.0 35.0 37.0 32.0 37.0 8 35.405127441491075 37.0 35.0 37.0 33.0 37.0 9 37.18659072295436 39.0 37.0 39.0 34.0 39.0 10 36.62872459236095 38.0 35.0 39.0 32.0 39.0 11 36.37496091132455 38.0 35.0 39.0 32.0 39.0 12 36.61426054153327 39.0 35.0 39.0 32.0 39.0 13 33.04506489961035 38.0 35.0 39.0 10.0 39.0 14 35.32175563993746 39.0 35.0 41.0 17.0 41.0 15 36.69480306753034 39.0 35.0 41.0 30.0 41.0 16 37.31757129029857 39.0 36.0 41.0 32.0 41.0 17 37.481091008363734 39.0 36.0 41.0 32.0 41.0 18 37.583217928672475 39.0 36.0 41.0 33.0 41.0 19 37.58632020450202 39.0 36.0 41.0 32.0 41.0 20 37.560695406149954 39.0 36.0 41.0 32.0 41.0 21 37.50605067877795 39.0 36.0 41.0 32.0 41.0 22 37.50898419080237 39.0 36.0 41.0 32.0 41.0 23 37.366056138783414 39.0 36.0 41.0 32.0 41.0 24 37.35945201399747 39.0 36.0 41.0 32.0 41.0 25 37.148556821284096 39.0 35.0 40.0 31.0 41.0 26 37.09731963368327 39.0 35.0 40.0 31.0 41.0 27 36.689072543617996 39.0 35.0 40.0 31.0 41.0 28 36.709224927406744 39.0 35.0 40.0 30.0 41.0 29 36.7477030749758 39.0 35.0 40.0 30.0 41.0 30 36.29733700642792 39.0 35.0 40.0 30.0 41.0 31 36.35629265629266 39.0 35.0 40.0 30.0 41.0 32 36.28991636264364 39.0 35.0 40.0 30.0 41.0 33 36.2707120343484 39.0 35.0 40.0 30.0 41.0 34 36.248824857915764 39.0 35.0 40.0 30.0 41.0 35 36.1805747896657 39.0 35.0 40.0 30.0 41.0 36 36.063815054724145 38.0 35.0 40.0 29.0 41.0 37 36.03585982676892 38.0 35.0 40.0 29.0 41.0 38 35.880947062765244 38.0 35.0 40.0 29.0 41.0 39 35.86035291489837 38.0 35.0 40.0 29.0 41.0 40 35.75109820564366 38.0 35.0 40.0 28.0 41.0 41 35.59140297322116 38.0 34.0 40.0 28.0 41.0 42 35.53236790509518 38.0 34.0 40.0 28.0 41.0 43 35.435805226714315 38.0 34.0 40.0 27.0 41.0 44 35.33292879656516 38.0 34.0 40.0 27.0 41.0 45 35.27505025686844 38.0 34.0 40.0 27.0 41.0 46 34.09900975355521 37.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 6.0 10 6.0 11 14.0 12 10.0 13 4.0 14 9.0 15 14.0 16 38.0 17 75.0 18 115.0 19 220.0 20 431.0 21 717.0 22 1113.0 23 1484.0 24 2099.0 25 2809.0 26 3632.0 27 4739.0 28 6073.0 29 7650.0 30 9651.0 31 12493.0 32 16099.0 33 21098.0 34 29294.0 35 36941.0 36 44578.0 37 60108.0 38 74072.0 39 67333.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.99516044970591 24.482168118531757 22.18325763780309 18.339413793959245 2 25.725312088948453 28.122750850023575 23.644553644553646 22.507383416474326 3 28.18876727967637 28.706723252177795 22.669694487876306 20.434814980269525 4 24.950984041893133 27.909314272950635 22.565706202069837 24.573995483086392 5 21.219566674112126 35.242349787804336 19.33933933933934 24.198744198744198 6 20.03697912788822 40.99173553719008 21.271188543915816 17.700096791005883 7 81.8849427940337 5.983669620033257 7.830144193780557 4.301243392152483 8 81.08331471967836 6.924527833618743 6.899213262849627 5.092944183853275 9 74.20792693519967 8.569230387412205 9.789045243590698 7.433797433797435 10 34.46479537388628 28.49725758816668 18.089742635197183 18.94820440274986 11 28.115305388032663 29.114734569280028 23.268805996078722 19.501154046608594 12 23.342267887722436 24.558856377038197 30.24272206090388 21.856153674335495 13 21.495296949842405 30.442756806393167 28.810959720050626 19.2509865237138 14 22.13337304246395 28.779936961755144 27.16253443526171 21.924155560519196 15 20.03151912242821 27.630109448291268 30.46410046410046 21.874270965180056 16 22.875437420891966 26.235326235326234 28.06591715682625 22.82331918695555 17 22.319013228104136 27.586925768743953 26.124885215794308 23.969175787357607 18 23.167299530935896 26.023130568585113 27.192564465291735 23.617005435187252 19 24.927406745588566 27.82542873451964 24.77452659270841 22.47263792718338 20 25.686347504529323 26.29588265951902 25.591293773111957 22.426476062839697 21 26.50137741046832 28.218549127640035 25.211078847442487 20.06899461444916 22 24.129997766361402 25.420792693519967 25.532226441317352 24.916983098801282 23 22.91862110043928 28.019010746283474 25.969026878117784 23.093341275159457 24 22.780383689474597 26.911374184101454 27.143672598218053 23.16456952820589 25 23.921773012682106 28.400714764351125 24.36304072667709 23.31447149628968 26 22.202615838979476 27.285632740178194 26.093862457498822 24.417888963343508 27 24.022286749559477 27.050107959198865 25.340381704018068 23.587223587223587 28 22.502419775147047 27.51520115156479 27.183381728836274 22.79899734445189 29 24.66904921450376 27.798128707219615 24.236219690765147 23.296602387511477 30 25.479611843248207 26.764698582880403 25.123718760082397 22.631970813788996 31 25.935770481225028 26.810115901024993 24.312411585138857 22.941702032611126 32 24.671531035167398 29.075769984860894 24.545206363388182 21.707492616583526 33 23.108728563274017 27.893926984836074 24.160027796391432 24.837316655498473 34 24.631077358350087 27.79515052242325 25.3937408482863 22.18003127094036 35 24.992182264909538 27.47822202367657 23.742833742833742 23.78676196858015 36 24.730102002829277 29.02737448191994 23.595909959546326 22.646613555704466 37 24.075397711761347 28.57344948254039 25.194450648996103 22.156702156702156 38 22.2477849750577 30.14245650609287 25.342118978482613 22.26763954036681 39 25.059191422827787 26.287196287196284 24.528329982875437 24.12528230710049 40 23.99573126845854 27.617203980840344 26.874395056213242 21.512669694487876 41 22.22668949941677 27.16154170699625 26.453230089593728 24.158538703993248 42 23.441292532201626 27.36132827041918 26.59717568808478 22.600203509294417 43 23.545777182140817 25.302658029930758 27.042166133075224 24.1093986548532 44 23.797433797433797 25.910207728389544 26.452485543394634 23.839872930782022 45 23.602610875338147 25.02345320527139 26.833693197329563 24.540242722060903 46 24.709006527188347 26.886059613332343 25.304147122328942 23.100786737150372 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2294.0 1 1982.0 2 1670.0 3 2169.5 4 2669.0 5 1883.0 6 1097.0 7 1097.0 8 1081.5 9 1066.0 10 1068.5 11 1071.0 12 1142.0 13 1213.0 14 1138.0 15 1063.0 16 1070.5 17 1078.0 18 1207.5 19 1337.0 20 1337.0 21 1285.0 22 1233.0 23 1545.5 24 1858.0 25 2149.0 26 2440.0 27 2755.5 28 3071.0 29 4287.0 30 5503.0 31 5503.0 32 6516.5 33 7530.0 34 8423.5 35 9317.0 36 10169.5 37 11022.0 38 12756.0 39 14490.0 40 16266.0 41 18042.0 42 20878.5 43 23715.0 44 23715.0 45 26765.5 46 29816.0 47 34111.0 48 38406.0 49 37478.5 50 36551.0 51 34739.5 52 32928.0 53 30598.0 54 28268.0 55 26688.5 56 25109.0 57 25109.0 58 23738.5 59 22368.0 60 21162.0 61 19956.0 62 18304.5 63 16653.0 64 14528.0 65 12403.0 66 10748.0 67 9093.0 68 7912.0 69 6731.0 70 6731.0 71 5634.5 72 4538.0 73 3864.5 74 3191.0 75 2531.0 76 1871.0 77 1511.0 78 1151.0 79 878.0 80 605.0 81 605.0 82 430.0 83 255.0 84 193.5 85 132.0 86 99.0 87 66.0 88 53.5 89 41.0 90 26.0 91 11.0 92 8.0 93 5.0 94 5.0 95 4.0 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 402930.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.77429075741396 #Duplication Level Percentage of deduplicated Percentage of total 1 75.58930489547426 27.797430763769842 2 8.8605951146263 6.516842020579785 3 3.3612030645324786 3.7081757636948454 4 1.9793081468710874 2.9115061316622226 5 1.2213227150863826 2.245663831661044 6 0.9227269524886388 2.0359577544311813 7 0.7388007679030257 1.90182119754666 8 0.6115121866915361 1.799034156407724 9 0.5787908825514834 1.9156161782419634 >10 6.03368977398241 42.61666638348384 >50 0.06827168078310614 1.739800775709649 >100 0.028390203890004528 2.12136874123534 >500 0.002027871706428895 0.47627636608939666 >1k 0.00405574341285779 2.2138399354865173 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2043 0.5070359615814161 No Hit ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA 1662 0.4124785942967761 No Hit GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG 1572 0.3901422083240265 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA 1251 0.3104757650212196 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCG 1220 0.30278212096393914 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1158 0.2873948328493783 No Hit GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT 809 0.20077929168838257 No Hit CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT 555 0.13774104683195593 No Hit CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT 552 0.13699650063286425 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC 447 0.1109373836646564 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 443 0.10994465539920086 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1702528975256248 0.0 2 0.0 0.0 0.0 0.6909388727570546 0.0 3 0.0 0.0 0.0 0.8939518030427122 0.0 4 0.0 0.0 0.0 1.2463703372794281 0.0 5 0.0 0.0 0.0 2.2557268011813467 0.0 6 0.0 0.0 0.0 3.086143995234904 0.0 7 0.0 0.0 0.0 3.701635519817338 0.0 8 0.0 0.0 0.0 4.86585759313032 0.0 9 0.0 0.0 0.0 5.292482565209838 0.0 10 0.0 0.0 0.0 6.408805499714591 0.0 11 0.0 0.0 0.0 7.229791775246321 0.0 12 0.0 0.0 0.0 8.273397364306454 0.0 13 0.0 0.0 0.0 8.63276499640136 0.0 14 0.0 0.0 0.0 8.808974263519717 0.0 15 0.0 0.0 0.0 9.08693817784727 0.0 16 0.0 0.0 0.0 9.610602337875065 0.0 17 0.0 0.0 0.0 10.176705631251085 0.0 18 0.0 0.0 0.0 10.9277045640682 0.0 19 0.0 0.0 0.0 11.261013079194898 0.0 20 0.0 0.0 0.0 11.587620678529769 0.0 21 0.0 0.0 0.0 11.958652867743776 0.0 22 0.0 0.0 0.0 12.305859578586851 0.0 23 0.0 0.0 0.0 12.692527237981784 0.0 24 0.0 0.0 0.0 13.000521182339364 0.0 25 0.0 0.0 0.0 13.263345990618719 0.0 26 0.0 0.0 0.0 13.503586230858959 0.0 27 0.0 0.0 0.0 13.78800287891197 0.0 28 0.0 0.0 0.0 14.059017695381332 0.0 29 0.0 0.0 0.0 14.350135259226168 0.0 30 0.0 0.0 0.0 14.730846549028367 0.0 31 0.0 0.0 0.0 15.012533194351375 0.0 32 0.0 0.0 0.0 15.321271684908048 0.0 33 0.0 0.0 0.0 15.597746506837415 0.0 34 0.0 0.0 0.0 15.868761323306778 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGCGA 20 0.0012547983 40.000004 1 CGCATCG 20 0.0012547983 40.000004 21 CCATGCG 20 0.0012547983 40.000004 1 GGTATAC 20 0.0012547983 40.000004 8 GCATCGC 20 0.0012547983 40.000004 22 CTCACGA 25 7.7856326E-5 40.0 24 TACGCAT 135 0.0 40.0 16 CATAGCG 25 7.7856326E-5 40.0 1 CGTACGG 25 7.7856326E-5 40.0 2 TAATGGC 25 7.7856326E-5 40.0 15 TCGCGAG 35 3.0497904E-7 40.0 1 CTACGCA 150 0.0 37.333332 15 ACGCATT 145 0.0 37.241383 17 TCGTTCA 70 0.0 37.142857 16 TAGGGTA 130 0.0 36.923077 5 TTACACG 190 0.0 36.842106 34 GCACTTA 430 0.0 36.744183 36 TACGCGG 55 2.1645974E-10 36.36364 2 CACCGCT 155 0.0 36.129032 25 GTCTTAC 205 0.0 36.09756 31 >>END_MODULE