FastQCFastQC Report
Sat 18 Jun 2016
SRR3553799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773372
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34670.44829655068970686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28320.366188587122368No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA17170.22201476133089895No Hit
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT7960.10292588818834922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG304.8654456E-640.0000041
TTGATCG200.001255513940.00000430
TCGTTAG200.001255513940.0000041
TCTATAG200.001255513940.0000041
CTATACG257.792287E-540.01
TAGAACG353.054647E-740.01
CGTTCGA257.792287E-540.014
CGCATTT2200.038.1818218
CGAATAT950.037.89473714
CGTAAGG1400.037.1428572
CGTATGG601.4551915E-1136.6666682
CGCTACA2250.036.44444328
TTCACGG503.421519E-936.02
TACGAAT1000.036.012
CGTTTTT14600.035.7534261
ACGCATT2350.035.7446817
TCGTTCA455.4385964E-835.55555716
ATTAGTC900.035.55555731
CGTTCAT455.4385964E-835.55555717
AAGGGAT5300.035.0943375
Produced by FastQC (version 0.11.5)