##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553799_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	773372
Sequences flagged as poor quality	0
Sequence length	46
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.752447722441463	31.0	31.0	34.0	30.0	34.0
2	31.909577796972222	31.0	31.0	34.0	30.0	34.0
3	31.848583863910253	33.0	31.0	34.0	30.0	34.0
4	35.627917742043934	37.0	35.0	37.0	33.0	37.0
5	33.74745271357122	37.0	35.0	37.0	30.0	37.0
6	34.55374515756971	37.0	35.0	37.0	30.0	37.0
7	35.435192895527635	37.0	35.0	37.0	32.0	37.0
8	35.53100836337494	37.0	35.0	37.0	33.0	37.0
9	37.26960634726884	39.0	37.0	39.0	34.0	39.0
10	36.71190578402114	39.0	37.0	39.0	32.0	39.0
11	36.4756145813399	39.0	35.0	39.0	32.0	39.0
12	36.69426614876153	39.0	35.0	39.0	32.0	39.0
13	33.12515193205857	38.0	35.0	39.0	10.0	39.0
14	35.406660701447684	39.0	35.0	41.0	17.0	41.0
15	36.824097588224035	39.0	35.0	41.0	30.0	41.0
16	37.43089224849103	39.0	36.0	41.0	32.0	41.0
17	37.6046016664684	39.0	36.0	41.0	33.0	41.0
18	37.70578324532049	39.0	37.0	41.0	33.0	41.0
19	37.690688310412064	39.0	37.0	41.0	33.0	41.0
20	37.67177244586046	39.0	36.0	41.0	33.0	41.0
21	37.57374070951625	39.0	36.0	41.0	32.0	41.0
22	37.54295604185308	39.0	36.0	41.0	32.0	41.0
23	37.42596964979337	39.0	36.0	41.0	32.0	41.0
24	37.38808232002193	39.0	36.0	41.0	32.0	41.0
25	37.173365469657554	39.0	35.0	41.0	31.0	41.0
26	37.145484967130955	39.0	35.0	41.0	32.0	41.0
27	36.75855215859897	39.0	35.0	40.0	31.0	41.0
28	36.732964213858274	39.0	35.0	40.0	31.0	41.0
29	36.76837925345112	39.0	35.0	40.0	31.0	41.0
30	36.29926865725679	39.0	35.0	40.0	30.0	41.0
31	36.41310649984742	39.0	35.0	40.0	30.0	41.0
32	36.35154750883145	39.0	35.0	40.0	30.0	41.0
33	36.32991884888514	39.0	35.0	40.0	30.0	41.0
34	36.27152004468742	39.0	35.0	40.0	30.0	41.0
35	36.18611095307303	39.0	35.0	40.0	30.0	41.0
36	36.06202707106024	38.0	35.0	40.0	29.0	41.0
37	35.99019359376859	38.0	35.0	40.0	29.0	41.0
38	35.85755238100164	38.0	35.0	40.0	29.0	41.0
39	35.782390621848215	38.0	35.0	40.0	28.0	41.0
40	35.67090998898331	38.0	35.0	40.0	28.0	41.0
41	35.49209823991559	38.0	34.0	40.0	27.0	41.0
42	35.4090554092985	38.0	34.0	40.0	27.0	41.0
43	35.313645955633255	38.0	34.0	40.0	27.0	41.0
44	35.23333660903162	38.0	34.0	40.0	27.0	41.0
45	35.17992246939377	38.0	34.0	40.0	27.0	41.0
46	34.05347749853887	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	17.0
10	19.0
11	11.0
12	17.0
13	14.0
14	23.0
15	33.0
16	58.0
17	124.0
18	241.0
19	480.0
20	766.0
21	1266.0
22	1844.0
23	2728.0
24	3853.0
25	5240.0
26	7176.0
27	9259.0
28	11853.0
29	14526.0
30	18108.0
31	22854.0
32	29829.0
33	40085.0
34	56056.0
35	70964.0
36	86527.0
37	115364.0
38	142895.0
39	131138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.51177958343462	23.813378296602412	22.901785945185498	20.77305617477747
2	26.022405776262914	26.749869403081572	23.62666349441149	23.601061326244032
3	28.21203767397837	26.388077147866746	23.399347274015607	22.000537904139275
4	25.153095793486184	28.114930460373532	22.04553565425177	24.686438091888512
5	21.102910371722793	35.39021324795829	19.565487242879236	23.941389137439682
6	20.610391894198393	39.56763368728115	21.35143242837858	18.47054199014187
7	83.5632787326151	5.700102925888189	6.845218083923391	3.8914002575733284
8	83.24467397319789	5.214696161743637	6.880776650822631	4.65985321423584
9	75.99395892274352	8.06597601154425	10.012128703909633	5.927936361802599
10	35.06669494111501	28.77916449005136	16.764894513894994	19.38924605493863
11	28.543572821358932	28.310308622499907	23.180694413555184	19.965424142585974
12	24.35994579581366	24.41735671837098	30.078668480369085	21.14402900544628
13	21.816409179541026	30.617865658441218	27.529313189512937	20.036411972504823
14	21.932006847933465	28.058036753334747	27.58245708404235	22.427499314689438
15	21.30333138515488	26.67707131884785	29.789932917147244	22.22966437885002
16	23.49580796822228	24.86578257294032	28.469481698328874	23.168927760508527
17	22.582146754731227	25.797287721820805	26.642676486865312	24.977889036582653
18	24.500757720734654	25.239082873442538	26.942402879856008	23.317756525966804
19	26.009087476660646	26.954686748421196	24.579374479551884	22.456851295366267
20	27.61349001515441	24.376367388527125	24.87845435314441	23.131688243174047
21	27.625773883719607	26.5098814024816	24.62217406371061	21.242170650088184
22	25.606435195481602	24.28805283873737	25.128269448596534	24.977242517184486
23	24.95513155376714	25.6822072689469	25.166026181449546	24.196634995836416
24	22.95350749703894	25.802201269246883	27.139591296297255	24.104699937416925
25	24.494809742271507	26.602721588058525	24.90250487475626	23.999963794913704
26	23.204874239046667	26.72413793103448	25.668242449946465	24.402745379972384
27	24.214996146744387	25.629968501574922	25.47687270808873	24.678162643591957
28	22.95635218239088	27.07196536724888	26.545181361621573	23.426501088738664
29	23.977464919857454	27.093171203508792	24.857895036282667	24.071468840351084
30	23.935053247337635	26.41186906171933	25.44390021878216	24.209177472160874
31	24.333438500488768	27.7961963970767	24.101855252064983	23.76850985036955
32	24.510972727225706	28.417113627077267	24.06448643085087	23.007427214846153
33	23.90699430545714	27.106877414749952	24.06655529292501	24.919572986867898
34	23.448482748276376	27.65150535576669	25.110942728725632	23.789069167231293
35	25.508940070237866	25.92775533637111	24.735185654510378	23.828118938880642
36	25.27425352870287	28.71296090367895	23.368443646783177	22.644341920834993
37	25.18322359744082	27.793222407845125	24.12176804952856	22.901785945185498
38	25.629709893815654	27.028777871451254	24.31559456509933	23.02591766963376
39	26.332347175744665	25.333345401695436	24.25857155418091	24.075735868378995
40	25.507259119802633	25.36308529401116	26.98765923772777	22.14199634845844
41	23.519987793713764	25.698628861660367	26.776893913924994	24.00448943070088
42	24.56385801399585	26.851243644714316	25.358301050464714	23.22659729082511
43	23.698427147608136	24.670145803054673	27.518451663623715	24.112975385713472
44	24.44140723998283	25.69552556854916	25.76030681224559	24.102760379222417
45	24.566056179949623	24.4582167443352	25.931117237241587	25.04460983847359
46	24.20064341610506	25.652596680510804	25.70082702761414	24.445932875770005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3191.0
1	2876.5
2	2562.0
3	4769.5
4	6977.0
5	4676.5
6	2376.0
7	2376.0
8	2174.5
9	1973.0
10	2076.5
11	2180.0
12	2104.5
13	2029.0
14	2066.5
15	2104.0
16	2035.0
17	1966.0
18	2072.5
19	2179.0
20	2179.0
21	2246.5
22	2314.0
23	2843.0
24	3372.0
25	4363.5
26	5355.0
27	5804.5
28	6254.0
29	8407.5
30	10561.0
31	10561.0
32	11760.5
33	12960.0
34	15197.0
35	17434.0
36	18921.0
37	20408.0
38	23305.5
39	26203.0
40	29831.5
41	33460.0
42	36803.5
43	40147.0
44	40147.0
45	45757.5
46	51368.0
47	55604.0
48	59840.0
49	62151.5
50	64463.0
51	61350.5
52	58238.0
53	54988.0
54	51738.0
55	50741.0
56	49744.0
57	49744.0
58	48413.0
59	47082.0
60	45165.5
61	43249.0
62	40533.0
63	37817.0
64	33833.5
65	29850.0
66	26775.0
67	23700.0
68	20488.0
69	17276.0
70	17276.0
71	14695.5
72	12115.0
73	10298.5
74	8482.0
75	7116.0
76	5750.0
77	4565.5
78	3381.0
79	2594.5
80	1808.0
81	1808.0
82	1350.5
83	893.0
84	622.0
85	351.0
86	247.0
87	143.0
88	86.0
89	29.0
90	32.5
91	36.0
92	23.0
93	10.0
94	10.0
95	6.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	773372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.356853003077724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57855144389139	26.30998035146402
2	8.372054179456214	5.752748695547593
3	3.263262593874484	3.3634629974456334
4	1.8138010544098244	2.4926598481274294
5	1.1516506422089894	1.9783545912637157
6	0.8567168090446389	1.766045608416748
7	0.661791494617512	1.5915951169482676
8	0.5355393058964935	1.4719556168044883
9	0.4752265894210717	1.4694561068304361
>10	6.055680284522046	45.58321066429576
>50	0.19430417164025562	4.098427552867384
>100	0.03727928791535539	2.427308193819803
>500	0.0030124677103317474	0.657735574958953
>1k	0.0011296753913744052	1.0370590812098675
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3467	0.44829655068970686	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2832	0.366188587122368	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	1717	0.22201476133089895	No Hit
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT	796	0.10292588818834922	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10486544638285328	0.0
2	0.0	0.0	0.0	0.43174565409660554	0.0
3	0.0	0.0	0.0	0.6240205231117755	0.0
4	0.0	0.0	0.0	0.8336221119978484	0.0
5	0.0	0.0	0.0	1.342820789994983	0.0
6	0.0	0.0	0.0	2.1168338134817395	0.0
7	0.0	0.0	0.0	2.631721862182753	0.0
8	0.0	0.0	0.0	3.66188587122368	0.0
9	0.0	0.0	0.0	4.137982756034612	0.0
10	0.0	0.0	0.0	4.873333919510921	0.0
11	0.0	0.0	0.0	5.546231309124199	0.0
12	0.0	0.0	0.0	6.118142368743632	0.0
13	0.0	0.0	0.0	6.4278251604661145	0.0
14	0.0	0.0	0.0	6.593463430276762	0.0
15	0.0	0.0	0.0	6.768411579421029	0.0
16	0.0	0.0	0.0	7.160331638590484	0.0
17	0.0	0.0	0.0	7.600352740983641	0.0
18	0.0	0.0	0.0	8.196055714455657	0.0
19	0.0	0.0	0.0	8.434104156861123	0.0
20	0.0	0.0	0.0	8.704995784693525	0.0
21	0.0	0.0	0.0	8.987783369452217	0.0
22	0.0	0.0	0.0	9.266821141701536	0.0
23	0.0	0.0	0.0	9.566288926932964	0.0
24	0.0	0.0	0.0	9.80472528097733	0.0
25	0.0	0.0	0.0	10.018593897891312	0.0
26	0.0	0.0	0.0	10.214101363897322	0.0
27	0.0	0.0	0.0	10.439607329978328	0.0
28	0.0	0.0	0.0	10.659423925355457	0.0
29	0.0	0.0	0.0	10.890489958260707	0.0
30	0.0	0.0	0.0	11.18685445038093	0.0
31	0.0	0.0	0.0	11.409774338869264	0.0
32	0.0	0.0	0.0	11.664502981747464	0.0
33	1.2930387963360453E-4	0.0	0.0	11.92440377981101	0.0
34	1.2930387963360453E-4	0.0	0.0	12.169563935596324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	30	4.8654456E-6	40.000004	1
TTGATCG	20	0.0012555139	40.000004	30
TCGTTAG	20	0.0012555139	40.000004	1
TCTATAG	20	0.0012555139	40.000004	1
CTATACG	25	7.792287E-5	40.0	1
TAGAACG	35	3.054647E-7	40.0	1
CGTTCGA	25	7.792287E-5	40.0	14
CGCATTT	220	0.0	38.18182	18
CGAATAT	95	0.0	37.894737	14
CGTAAGG	140	0.0	37.142857	2
CGTATGG	60	1.4551915E-11	36.666668	2
CGCTACA	225	0.0	36.444443	28
TTCACGG	50	3.421519E-9	36.0	2
TACGAAT	100	0.0	36.0	12
CGTTTTT	1460	0.0	35.753426	1
ACGCATT	235	0.0	35.74468	17
TCGTTCA	45	5.4385964E-8	35.555557	16
ATTAGTC	90	0.0	35.555557	31
CGTTCAT	45	5.4385964E-8	35.555557	17
AAGGGAT	530	0.0	35.094337	5
>>END_MODULE