FastQCFastQC Report
Sat 18 Jun 2016
SRR3553797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922916
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85010.921102245491464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26340.2853997546905677No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTC9430.1021761460414599No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCT9380.10163438492777242No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGTCG200.0012556440.0000041
CGTCATA200.0012556440.00000438
CGATTCA304.8664715E-640.010
TCGATAG257.793458E-540.01
CAATACG257.793458E-540.01
CGTTTTT34950.037.8254621
CGTACGG750.037.3333322
AATGCGG1100.036.3636362
CACGACC2900.035.17241327
CGTTATT2050.035.1219521
TATTGCG408.6974615E-735.0000041
CGGGATC1600.035.0000046
CCAACGG750.034.6666682
ACACGAC2950.034.5762726
TCGTTAG351.4007694E-534.2857131
ACTTACT7000.034.28571338
CTAACGG653.45608E-1133.8461532
CCCTCGT1250.033.614
GCGAATT302.2779153E-433.3333329
GTCGTAA302.2779153E-433.33333232
Produced by FastQC (version 0.11.5)