##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553797_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	922916
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.726370547265407	31.0	31.0	34.0	30.0	34.0
2	31.8967338305978	31.0	31.0	34.0	30.0	34.0
3	31.75812316613863	31.0	31.0	34.0	30.0	34.0
4	35.586298211321505	37.0	35.0	37.0	33.0	37.0
5	33.7085390219695	37.0	35.0	37.0	30.0	37.0
6	34.54072201587143	37.0	35.0	37.0	30.0	37.0
7	35.46580403850405	37.0	35.0	37.0	32.0	37.0
8	35.550903874242074	37.0	35.0	37.0	34.0	37.0
9	37.31424961751665	39.0	37.0	39.0	34.0	39.0
10	36.75350736145001	39.0	37.0	39.0	32.0	39.0
11	36.50802889970485	39.0	35.0	39.0	32.0	39.0
12	36.709571618652184	39.0	35.0	39.0	32.0	39.0
13	33.11123547538454	38.0	35.0	39.0	10.0	39.0
14	35.41921258272692	39.0	35.0	41.0	17.0	41.0
15	36.83437604289014	39.0	35.0	41.0	30.0	41.0
16	37.421331952203666	39.0	36.0	41.0	32.0	41.0
17	37.606352040705765	39.0	36.0	41.0	33.0	41.0
18	37.69351381924249	39.0	37.0	41.0	33.0	41.0
19	37.65240932002479	39.0	37.0	41.0	33.0	41.0
20	37.61122789072895	39.0	36.0	41.0	33.0	41.0
21	37.52038972127474	39.0	36.0	41.0	32.0	41.0
22	37.51020352881519	39.0	36.0	41.0	33.0	41.0
23	37.38978520255365	39.0	36.0	41.0	32.0	41.0
24	37.359967754378516	39.0	35.0	41.0	32.0	41.0
25	37.144915680300265	39.0	35.0	40.0	32.0	41.0
26	37.10494346181017	39.0	35.0	40.0	32.0	41.0
27	36.681229927750735	39.0	35.0	40.0	31.0	41.0
28	36.694732781748286	39.0	35.0	40.0	31.0	41.0
29	36.73627719099029	39.0	35.0	40.0	31.0	41.0
30	36.24670825947324	39.0	35.0	40.0	30.0	41.0
31	36.31865088480425	39.0	35.0	40.0	30.0	41.0
32	36.218971173974666	38.0	35.0	40.0	30.0	41.0
33	36.13243025367422	38.0	35.0	40.0	30.0	41.0
34	36.04037203819199	38.0	35.0	40.0	29.0	41.0
35	35.944786957859655	38.0	35.0	40.0	29.0	41.0
36	35.831960871845325	38.0	35.0	40.0	28.0	41.0
37	35.809287085715276	38.0	35.0	40.0	28.0	41.0
38	35.68932925640036	38.0	35.0	40.0	28.0	41.0
39	35.64776209319158	38.0	35.0	40.0	28.0	41.0
40	35.55095371626454	38.0	35.0	40.0	27.0	41.0
41	35.377054899904216	38.0	34.0	40.0	27.0	41.0
42	35.276546294570686	38.0	34.0	40.0	26.0	41.0
43	35.19048970870588	38.0	34.0	40.0	26.0	41.0
44	35.09540954973151	38.0	34.0	40.0	26.0	41.0
45	35.02941112733986	38.0	34.0	40.0	26.0	41.0
46	33.929663154609955	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	15.0
10	31.0
11	27.0
12	21.0
13	14.0
14	18.0
15	25.0
16	89.0
17	130.0
18	280.0
19	494.0
20	833.0
21	1442.0
22	2249.0
23	3254.0
24	4841.0
25	6511.0
26	9206.0
27	12351.0
28	15739.0
29	18572.0
30	22435.0
31	28026.0
32	35669.0
33	47550.0
34	67309.0
35	84272.0
36	101715.0
37	136410.0
38	169683.0
39	153700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.943427137464298	22.806192546233895	22.01457120691374	23.235809109388068
2	28.748553497826457	25.311295935924825	22.064629933818463	23.875520632430252
3	27.51182122750066	25.895531120925412	25.3860589696137	21.206588681960223
4	24.80940844020474	27.105066983344095	23.580585882138784	24.504938694312376
5	20.611734979131363	34.53640417979534	21.087401236948974	23.76445960412432
6	20.204764030529322	38.74913859982924	22.910535736730104	18.135561632911337
7	81.63418989377148	5.298965452977302	9.053261618608845	4.013583034642373
8	81.32636122897425	5.0634077207459836	9.212647738255702	4.397583312024063
9	74.66497492729566	7.309549298094301	11.98657299255837	6.038902782051671
10	34.01403811397787	27.212118979408746	19.174334392295727	19.599508514317662
11	28.21611067529439	26.883161631177703	24.838230131452914	20.062497562074988
12	23.150427557870923	24.438735486219766	31.0014129129845	21.409424042924815
13	21.22576702538476	29.957330894685974	29.705520329044028	19.111381750885236
14	22.128991154124535	26.253960273741058	29.914098357813714	21.702950214320698
15	21.306922840215144	25.638302944146595	31.79032544673621	21.264448768902046
16	23.149127331198073	25.25451937121038	30.528347108512584	21.068006189078964
17	22.612025363088296	25.38400027738169	28.62080622721895	23.383168132311067
18	23.76272596856052	24.689895938525282	28.874783837315636	22.67259425559856
19	25.25495278010133	26.293183778372033	27.037671900801374	21.414191540725266
20	26.855748518825116	24.585010986915385	27.000940497293364	21.558299996966138
21	26.645003445600686	26.225571991383834	26.754005781674607	20.375418781340883
22	24.831945702534142	24.897498797290325	26.425806899002723	23.844748601172803
23	23.641263126871785	26.195558425685544	27.145807419093394	23.017371028349277
24	22.69979066350567	25.326573599330814	28.692643750893904	23.280991986269605
25	24.0623198644297	26.148208504349256	27.003866007307277	22.785605623913767
26	22.77596227609013	26.325797797416016	27.22295420168249	23.67528572481136
27	23.700531792709196	25.518357033576187	27.36749606681431	23.413615106900306
28	22.94109106354208	26.638068903345484	27.970909595239434	22.449930437873004
29	23.85005786008694	26.162185941082395	26.586493245322433	23.40126295350823
30	23.85265831343264	26.298709741731642	27.03908047969696	22.809551465138757
31	25.248776703405294	26.067702802855298	25.493869431237513	23.189651062501895
32	23.998717109682786	26.966159433794623	26.601987613173893	22.43313584334869
33	24.369498415890504	25.77027595144087	26.074420640665025	23.785804992003605
34	22.906093295597866	26.215711939114716	27.680525638302946	23.197669126984472
35	24.047583962137402	25.590519613919362	26.60361289651496	23.758283527428283
36	23.025605797277326	28.52307252230972	25.547612133715308	22.90370954669764
37	23.844315192281854	27.13269680014216	26.17757195671112	22.845416050864866
38	23.52207568186054	27.28330638974728	26.515522539429377	22.679095388962807
39	23.34665343324853	25.04084878797204	26.690403026927694	24.922094751851738
40	23.726427973943455	25.294501341400515	28.492733899943225	22.4863367847128
41	21.71660259438562	26.38766691659913	27.827667956780466	24.068062532234784
42	22.626544560935123	26.867883967771718	27.615622656883183	22.88994881440998
43	23.10156070541631	24.792505493457693	28.78355126577067	23.32238253535533
44	23.285326075179107	25.084298029289776	27.35568567453593	24.274690220995193
45	23.75579142630532	24.235466716364222	27.390141681366455	24.61860017596401
46	23.4266173736288	25.170871455257032	26.63904407335012	24.763467097764043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2901.0
1	2541.5
2	2182.0
3	9046.5
4	15911.0
5	10477.0
6	5043.0
7	5043.0
8	4605.0
9	4167.0
10	4001.5
11	3836.0
12	3723.5
13	3611.0
14	3475.5
15	3340.0
16	3235.5
17	3131.0
18	3116.0
19	3101.0
20	3101.0
21	3200.5
22	3300.0
23	3799.0
24	4298.0
25	5173.0
26	6048.0
27	7172.0
28	8296.0
29	10090.5
30	11885.0
31	11885.0
32	13852.5
33	15820.0
34	18019.5
35	20219.0
36	22675.5
37	25132.0
38	27684.5
39	30237.0
40	34134.0
41	38031.0
42	42469.5
43	46908.0
44	46908.0
45	54170.0
46	61432.0
47	68479.5
48	75527.0
49	78639.5
50	81752.0
51	79600.5
52	77449.0
53	71123.0
54	64797.0
55	61901.0
56	59005.0
57	59005.0
58	56490.0
59	53975.0
60	50945.0
61	47915.0
62	44178.0
63	40441.0
64	35562.5
65	30684.0
66	27490.0
67	24296.0
68	20776.0
69	17256.0
70	17256.0
71	14482.5
72	11709.0
73	9935.5
74	8162.0
75	6573.0
76	4984.0
77	4000.0
78	3016.0
79	2337.0
80	1658.0
81	1658.0
82	1268.0
83	878.0
84	580.0
85	282.0
86	222.5
87	163.0
88	124.0
89	85.0
90	59.0
91	33.0
92	25.0
93	17.0
94	17.0
95	9.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	922916.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.172719573229266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92994121528477	30.135550140113626
2	8.482453423922053	6.64561538536554
3	3.252996160625612	3.8228611911893564
4	1.8773167438709455	2.941584094311377
5	1.175066459273944	2.301527444452282
6	0.8808018938410301	2.070204335220236
7	0.70244185581396	1.9261590484009303
8	0.5815620718707557	1.8225094364655456
9	0.5310768227249107	1.8723351103600068
>10	5.49139752913525	40.6455469464764
>50	0.06473604688906129	1.6773797921183164
>100	0.02697301495399653	2.1842166482012364
>500	0.0026973014947731196	0.7779792559408862
>1k	2.697301494773119E-4	0.2783101127459316
>5k	2.697301494773119E-4	0.8982210586382553
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8501	0.921102245491464	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2634	0.2853997546905677	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTC	943	0.1021761460414599	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCT	938	0.10163438492777242	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08538155151714782	0.0
2	0.0	0.0	0.0	0.3494359183284286	0.0
3	0.0	0.0	0.0	0.5901945572511474	0.0
4	0.0	0.0	0.0	0.8201179739001165	0.0
5	0.0	0.0	0.0	1.371305730965765	0.0
6	0.0	0.0	0.0	2.284281559751917	0.0
7	0.0	0.0	0.0	2.916733483870688	0.0
8	0.0	0.0	0.0	4.0516146648232345	0.0
9	0.0	0.0	0.0	4.624364514213645	0.0
10	0.0	0.0	0.0	5.450550212587061	0.0
11	0.0	0.0	0.0	6.25409029640834	0.0
12	0.0	0.0	0.0	6.883291653844987	0.0
13	0.0	0.0	0.0	7.250497336702365	0.0
14	0.0	0.0	0.0	7.415517771931573	0.0
15	0.0	0.0	0.0	7.620195120682705	0.0
16	0.0	0.0	0.0	8.029333113739495	0.0
17	0.0	0.0	0.0	8.480728473663909	0.0
18	0.0	0.0	0.0	9.062254853096057	0.0
19	0.0	0.0	0.0	9.334110579944436	0.0
20	0.0	0.0	0.0	9.625903115776516	0.0
21	0.0	0.0	0.0	9.946192286188559	0.0
22	0.0	0.0	0.0	10.276774917760664	0.0
23	0.0	0.0	0.0	10.622201803847805	0.0
24	0.0	0.0	0.0	10.908793432988483	0.0
25	0.0	0.0	0.0	11.172847799799765	0.0
26	0.0	0.0	0.0	11.412956325386059	0.0
27	0.0	0.0	0.0	11.68784591447109	0.0
28	0.0	0.0	0.0	11.959376584651258	0.0
29	1.0835222273749724E-4	0.0	0.0	12.2423925904416	0.0
30	1.0835222273749724E-4	0.0	0.0	12.612198726644678	0.0
31	1.0835222273749724E-4	0.0	0.0	12.884812919052221	0.0
32	1.0835222273749724E-4	0.0	0.0	13.208027599478175	0.0
33	1.0835222273749724E-4	0.0	0.0	13.49656956862813	0.0
34	1.0835222273749724E-4	0.0	0.0	13.770483987708523	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGTCG	20	0.00125564	40.000004	1
CGTCATA	20	0.00125564	40.000004	38
CGATTCA	30	4.8664715E-6	40.0	10
TCGATAG	25	7.793458E-5	40.0	1
CAATACG	25	7.793458E-5	40.0	1
CGTTTTT	3495	0.0	37.825462	1
CGTACGG	75	0.0	37.333332	2
AATGCGG	110	0.0	36.363636	2
CACGACC	290	0.0	35.172413	27
CGTTATT	205	0.0	35.121952	1
TATTGCG	40	8.6974615E-7	35.000004	1
CGGGATC	160	0.0	35.000004	6
CCAACGG	75	0.0	34.666668	2
ACACGAC	295	0.0	34.57627	26
TCGTTAG	35	1.4007694E-5	34.285713	1
ACTTACT	700	0.0	34.285713	38
CTAACGG	65	3.45608E-11	33.846153	2
CCCTCGT	125	0.0	33.6	14
GCGAATT	30	2.2779153E-4	33.333332	9
GTCGTAA	30	2.2779153E-4	33.333332	32
>>END_MODULE