FastQCFastQC Report
Sat 18 Jun 2016
SRR3553794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953528
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150631.579712394392194No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25230.26459632019196083No Hit
CGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTC16210.1700002516968563No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15010.15741540888154307No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA9980.10466394274735509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATACG304.8666407E-640.0000041
GCGTAAG257.793652E-540.0000041
CGTTTTT61850.038.4478571
ACGCATT1250.036.817
CGTTATT3600.036.111111
ACTAACG455.4407792E-835.5555571
CATAACG455.4407792E-835.5555571
CGCATTT1300.035.38461318
ACGGGTA2500.035.25
GTCAAGC2200.034.54545216
TCACACG351.4008176E-534.2857131
GCGATCG701.8189894E-1234.2857139
AACGGGA4050.034.0740784
TACGCAT1350.034.07407416
CGTAAGG2350.034.0425532
CACTTAC7250.033.93103437
CGACGGT653.45608E-1133.84615328
GAGTACG653.45608E-1133.8461531
GCACTTA7350.033.46938736
ACGTTAG302.2779709E-433.3333361
Produced by FastQC (version 0.11.5)