FastQCFastQC Report
Sat 18 Jun 2016
SRR3553789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406019
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49771.2258047037207618No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG35280.8689248532704135No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCG32350.7967607427238627No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA28950.7130208192227457No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT17060.42017738086148676No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.31180806809533546No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT10780.2655048162770708No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT10500.2586085872828612No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10280.2531901216445536No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9150.22535891177506473No Hit
ATATATATATACATATATATGTATATATACATATATATGTATATAT7860.19358699962317036No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC7600.1871833584142614No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGT6960.17142054928463937No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA6920.17043537371403802No Hit
ATATATATATATGTATATATACATATATATGTATATATACATATAT5880.14482080887840224No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATG5870.1445745149857519No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGT5440.13398387760178712No Hit
ACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT5230.1288117058561299No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAA4900.12068400739866852No Hit
ATATATATATGTATATATACATATATATGTATATATACATATATAT4670.11501924786771062No Hit
AAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG4160.10245825934254307No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC257.7857396E-540.08
TCCATAA200.0012548140.030
CAAACGG451.2169039E-940.02
TGCCACG200.0012548140.01
GCTATTC200.0012548140.010
CGTTGGA200.0012548140.028
CGGTTAC200.0012548140.026
AGCCACG304.8597285E-640.01
ATTAACG200.0012548140.01
CGTCTGA257.7857396E-540.028
CCTAACG257.7857396E-540.01
CATTATC200.0012548140.021
ATATACG200.0012548140.024
CTACGAA304.8597285E-640.011
TACGAAT304.8597285E-640.012
CGGGCTA850.040.06
ACTACGG200.0012548140.02
TCGTCGG200.0012548140.02
CGAATAT304.8597285E-640.014
ATGCGTA200.0012548140.012
Produced by FastQC (version 0.11.5)