##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553789_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406019
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.561380132456854	31.0	31.0	34.0	30.0	34.0
2	31.707461966065626	31.0	31.0	34.0	30.0	34.0
3	31.670567140946606	31.0	31.0	34.0	30.0	34.0
4	35.485110302719825	37.0	35.0	37.0	33.0	37.0
5	33.57189688167303	37.0	35.0	37.0	30.0	37.0
6	34.38120137234957	37.0	35.0	37.0	30.0	37.0
7	35.20140683071482	37.0	35.0	37.0	32.0	37.0
8	35.196483415800735	37.0	35.0	37.0	32.0	37.0
9	37.013519071767575	39.0	37.0	39.0	33.0	39.0
10	36.560850600587656	38.0	35.0	39.0	32.0	39.0
11	36.31302722286395	38.0	35.0	39.0	32.0	39.0
12	36.590006871599606	39.0	35.0	39.0	32.0	39.0
13	32.90067459897197	38.0	35.0	39.0	10.0	39.0
14	35.164344525748795	39.0	34.0	40.0	17.0	41.0
15	36.51098593908167	39.0	34.0	40.0	30.0	41.0
16	37.19811880724794	39.0	36.0	40.0	32.0	41.0
17	37.336129589994556	39.0	36.0	40.0	32.0	41.0
18	37.40984781500373	39.0	36.0	40.0	32.0	41.0
19	37.36021959563469	39.0	36.0	40.0	32.0	41.0
20	37.3289870671077	39.0	36.0	40.0	32.0	41.0
21	37.274721133740044	39.0	35.0	40.0	32.0	41.0
22	37.290762254968364	39.0	35.0	40.0	32.0	41.0
23	37.14407946426152	39.0	35.0	40.0	32.0	41.0
24	37.11949194495824	39.0	35.0	40.0	32.0	41.0
25	36.92238294267017	39.0	35.0	40.0	31.0	41.0
26	36.91195239631643	39.0	35.0	40.0	31.0	41.0
27	36.5030429610437	39.0	35.0	40.0	31.0	41.0
28	36.531967715796554	39.0	35.0	40.0	30.0	41.0
29	36.5472404000798	39.0	35.0	40.0	30.0	41.0
30	36.04063602934838	38.0	35.0	40.0	30.0	41.0
31	36.16342584952921	38.0	35.0	40.0	30.0	41.0
32	36.05027104642886	38.0	35.0	40.0	29.0	41.0
33	35.95043335410412	38.0	35.0	40.0	29.0	41.0
34	35.92573992842699	38.0	35.0	40.0	29.0	41.0
35	35.840761146645846	38.0	35.0	40.0	29.0	41.0
36	35.691172088005736	38.0	35.0	40.0	28.0	41.0
37	35.64063504417281	38.0	35.0	40.0	28.0	41.0
38	35.46970215679562	38.0	34.0	40.0	27.0	41.0
39	35.338841285752636	38.0	34.0	40.0	26.0	41.0
40	35.12066676682618	38.0	34.0	40.0	26.0	41.0
41	34.976077474206875	38.0	34.0	40.0	25.0	41.0
42	34.89543346493637	38.0	34.0	40.0	25.0	41.0
43	34.80176543462252	38.0	33.0	40.0	24.0	41.0
44	34.774830734522276	38.0	34.0	40.0	24.0	41.0
45	34.722444023555546	37.0	33.0	40.0	24.0	41.0
46	33.42281272551285	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	10.0
11	14.0
12	9.0
13	6.0
14	7.0
15	17.0
16	41.0
17	57.0
18	117.0
19	250.0
20	438.0
21	799.0
22	1301.0
23	1768.0
24	2530.0
25	3402.0
26	4288.0
27	5346.0
28	6720.0
29	8290.0
30	10794.0
31	13739.0
32	17624.0
33	23126.0
34	31097.0
35	39762.0
36	46447.0
37	59851.0
38	71097.0
39	57062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.61385058334709	25.93277654494002	19.806954846940663	18.64641802477224
2	24.290981456532823	32.0420965521318	23.018134619315845	20.648787372019537
3	25.242414763841104	32.87875690546502	22.40609429607974	19.472734034614145
4	23.089559848184443	28.06839088811115	26.43644755540997	22.40560170829444
5	21.00714498582579	36.99186491272576	19.094426615503217	22.906563485945238
6	17.996448442067983	41.63992325482305	23.57943840066598	16.78418990244299
7	74.2879027828747	9.562360382149604	11.314495134464151	4.835241700511552
8	72.52862550767328	10.320206689834713	10.764520872175932	6.3866469303160684
9	65.90085685645252	11.727037404653478	12.227260300626325	10.144845438267668
10	32.033229971996384	27.08961895871868	20.972417546962088	19.90473352232285
11	25.628603587516842	29.562902228713433	24.58948965442504	20.219004529344687
12	21.77681340035811	23.361960893455723	32.147510338186144	22.71371536800002
13	20.616522872082342	31.549016179045807	30.436260372051553	17.398200576820297
14	19.46362116058608	30.20425152517493	28.576002600863504	21.75612471337548
15	18.029944411468428	28.169863971883093	32.92382868782003	20.876362928828453
16	18.829168093118795	27.383940160435845	28.837320420965522	24.94957132547984
17	18.642969910275134	29.88160652580298	27.438863698496867	24.036559865425016
18	19.673956144909475	27.379999458153435	29.019577901526777	23.926466495410313
19	21.11674576805519	31.190904859132207	25.709880572091453	21.982468800721147
20	22.83439937539869	27.097500363283494	28.418374509567286	21.649725751750534
21	23.33634632862009	32.02707262468012	26.160105807856283	18.476475238843502
22	20.197330666791455	26.614271745903515	26.86228969580241	26.326107891502616
23	20.35027917412732	30.471234104807905	26.04237732716942	23.13610939389536
24	21.064777756705965	27.26153209578862	27.61102312945946	24.062667018045953
25	20.719966306995484	31.88126664023112	23.98927143803615	23.409495614737242
26	18.981624012669357	27.807319361901783	28.44965383393388	24.76140279149498
27	22.0923158768432	29.12252874865462	23.875976247416993	24.909179127085185
28	19.31880035170768	27.44773027863228	28.711217947928546	24.522251421731493
29	22.41914787239021	27.496989057162352	25.24783322947941	24.83602984096803
30	20.683514810883235	28.631911314495134	25.56358199985715	25.12099187476448
31	23.69445764853369	27.47753183964297	22.196005605648995	26.632004906174338
32	23.28905790123122	28.564180494016288	24.649585364231726	23.497176240520766
33	21.235213130420007	29.22400183242656	22.143298712621824	27.397486324531613
34	22.486878692869052	26.780520123442496	26.656388001546727	24.07621318214172
35	22.996214462869965	28.254096483169505	22.365702097685084	26.383986956275447
36	22.016457357906898	30.248584425851995	24.23778197572035	23.497176240520766
37	23.703077934776452	28.895692073523655	23.905285220642384	23.495944771057513
38	21.564508064893513	30.445373246079615	23.575005110598273	24.415113578428596
39	26.47289905152222	27.44625251527638	23.470083912329226	22.610764520872177
40	23.00754398193188	28.017900640117826	28.294734975456816	20.67982040249348
41	20.983747066024	29.410692603055523	25.377383817013488	24.228176513906984
42	20.582288021003944	28.552604681061723	28.045239262202017	22.81986803573232
43	22.417670109034308	26.58496277267813	26.394085005874107	24.603282112413456
44	22.675786108531867	24.55402333388339	27.206608557727595	25.56358199985715
45	22.294769456601784	24.372997322785388	26.785692295188156	26.546540925424672
46	24.746378864043308	26.091882399592137	26.052967964553382	23.108770771811173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5583.0
1	4481.0
2	3379.0
3	3449.5
4	3520.0
5	2693.5
6	1867.0
7	1867.0
8	2762.0
9	3657.0
10	2922.5
11	2188.0
12	1954.0
13	1720.0
14	1750.0
15	1780.0
16	1801.5
17	1823.0
18	1799.5
19	1776.0
20	1776.0
21	1630.0
22	1484.0
23	1805.0
24	2126.0
25	2488.0
26	2850.0
27	3438.5
28	4027.0
29	4683.5
30	5340.0
31	5340.0
32	5810.0
33	6280.0
34	7043.0
35	7806.0
36	8902.5
37	9999.0
38	11434.0
39	12869.0
40	14888.5
41	16908.0
42	21152.5
43	25397.0
44	25397.0
45	28775.0
46	32153.0
47	38866.0
48	45579.0
49	43270.5
50	40962.0
51	37205.0
52	33448.0
53	29984.0
54	26520.0
55	24444.0
56	22368.0
57	22368.0
58	20493.0
59	18618.0
60	17094.0
61	15570.0
62	14034.0
63	12498.0
64	10987.5
65	9477.0
66	8738.0
67	7999.0
68	7376.5
69	6754.0
70	6754.0
71	5699.5
72	4645.0
73	3816.5
74	2988.0
75	2528.0
76	2068.0
77	1527.0
78	986.0
79	738.5
80	491.0
81	491.0
82	392.5
83	294.0
84	213.0
85	132.0
86	98.5
87	65.0
88	39.0
89	13.0
90	11.0
91	9.0
92	6.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	406019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.27906171044831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14631134208257	27.987957899128713
2	9.293280846886326	6.743030186734327
3	3.5265512937622736	3.838199160343885
4	1.8511043393051894	2.6862531423252647
5	1.1607602561206931	2.1055646481419203
6	0.8250095330723736	1.795834305722447
7	0.6119360400754084	1.5540325752520174
8	0.5058732618353279	1.4682085827031708
9	0.39476009528007455	1.288937327173945
>10	4.381603115434365	35.12169586301978
>50	0.23816507112562074	5.362976143193856
>100	0.05239631564763656	3.4178974729570495
>500	0.006124244686087391	1.5036773931225271
>1k	0.006124244686087391	5.125735300181092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4977	1.2258047037207618	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	3528	0.8689248532704135	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCG	3235	0.7967607427238627	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	2895	0.7130208192227457	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	1706	0.42017738086148676	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1266	0.31180806809533546	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	1078	0.2655048162770708	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	1050	0.2586085872828612	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1028	0.2531901216445536	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	915	0.22535891177506473	No Hit
ATATATATATACATATATATGTATATATACATATATATGTATATAT	786	0.19358699962317036	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	760	0.1871833584142614	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGT	696	0.17142054928463937	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	692	0.17043537371403802	No Hit
ATATATATATATGTATATATACATATATATGTATATATACATATAT	588	0.14482080887840224	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATG	587	0.1445745149857519	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGT	544	0.13398387760178712	No Hit
ACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	523	0.1288117058561299	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAA	490	0.12068400739866852	No Hit
ATATATATATGTATATATACATATATATGTATATATACATATATAT	467	0.11501924786771062	No Hit
AAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	416	0.10245825934254307	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31082289252473405	0.0
2	0.0	0.0	0.0	1.3629904019270034	0.0
3	0.0	0.0	0.0	1.7053389127109815	0.0
4	0.0	0.0	0.0	2.330186518364904	0.0
5	0.0	0.0	0.0	4.341176151854962	0.0
6	0.0	0.0	0.0	5.614515576857241	0.0
7	0.0	0.0	0.0	6.702888288479111	0.0
8	0.0	0.0	0.0	8.60772525423687	0.0
9	0.0	0.0	0.0	9.31532760782131	0.0
10	0.0	0.0	0.0	11.373113080914932	0.0
11	0.0	0.0	0.0	12.715414795859306	0.0
12	0.0	0.0	0.0	14.916543314475431	0.0
13	0.0	0.0	0.0	15.518978175898173	0.0
14	0.0	0.0	0.0	15.86181927446745	0.0
15	0.0	0.0	0.0	16.3610569948697	0.0
16	0.0	0.0	0.0	17.181708245180644	0.0
17	0.0	0.0	0.0	18.005315022203394	0.0
18	0.0	0.0	0.0	19.228410493105002	0.0
19	0.0	0.0	0.0	19.767055236331306	0.0
20	0.0	0.0	0.0	20.32318684593578	0.0
21	0.0	0.0	0.0	20.8736536960093	0.0
22	0.0	0.0	0.0	21.415253965947407	0.0
23	0.0	0.0	0.0	21.941830308433843	0.0
24	0.0	0.0	0.0	22.36200768929533	0.0
25	0.0	0.0	0.0	22.731448528270846	0.0
26	2.4629389265034396E-4	0.0	0.0	23.073797039054824	0.0
27	2.4629389265034396E-4	0.0	0.0	23.43831200017733	0.0
28	2.4629389265034396E-4	0.0	0.0	23.800117728480686	0.0
29	2.4629389265034396E-4	0.0	0.0	24.180395498732818	0.0
30	2.4629389265034396E-4	0.0	0.0	24.681849864168917	0.0
31	2.4629389265034396E-4	0.0	0.0	25.067299806166705	0.0
32	2.4629389265034396E-4	0.0	0.0	25.460631152729306	0.0
33	2.4629389265034396E-4	0.0	0.0	25.84411074358589	0.0
34	2.4629389265034396E-4	0.0	0.0	26.177346380341806	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGC	25	7.7857396E-5	40.0	8
TCCATAA	20	0.00125481	40.0	30
CAAACGG	45	1.2169039E-9	40.0	2
TGCCACG	20	0.00125481	40.0	1
GCTATTC	20	0.00125481	40.0	10
CGTTGGA	20	0.00125481	40.0	28
CGGTTAC	20	0.00125481	40.0	26
AGCCACG	30	4.8597285E-6	40.0	1
ATTAACG	20	0.00125481	40.0	1
CGTCTGA	25	7.7857396E-5	40.0	28
CCTAACG	25	7.7857396E-5	40.0	1
CATTATC	20	0.00125481	40.0	21
ATATACG	20	0.00125481	40.0	24
CTACGAA	30	4.8597285E-6	40.0	11
TACGAAT	30	4.8597285E-6	40.0	12
CGGGCTA	85	0.0	40.0	6
ACTACGG	20	0.00125481	40.0	2
TCGTCGG	20	0.00125481	40.0	2
CGAATAT	30	4.8597285E-6	40.0	14
ATGCGTA	20	0.00125481	40.0	12
>>END_MODULE