FastQCFastQC Report
Sat 18 Jun 2016
SRR3553788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518396
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109830.7233290920155216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34910.2299136720591993No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT31960.21048527525098856No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG23100.152134225854125No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT22750.14982916182603223No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT21850.14390185432522215No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTC21210.1396868801024239No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC20450.13468159821285092No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC18040.11880958590512619No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG16430.10820629137589931No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATC200.001255896140.01
ACGCTAG353.0572483E-740.01
TATAGCG600.040.01
TACGTAG600.040.01
CGTTTTT49800.038.072291
GCGAAGT503.426976E-936.0000049
GCTACGA1000.036.00000410
CCGCACG455.4444172E-835.5555571
AACGGGA10450.035.40674
GCGATCG850.035.294129
CGTAAGG3350.035.223882
GCGTTAG800.035.01
CCGTAAG701.8189894E-1234.2857131
TCGATAG1000.034.0000041
TGCACGG3900.033.8461532
GCGATCA2850.033.684219
CGCACGG4100.033.658542
TGGGCGA12450.033.57436
ACGGGAA12050.033.526975
TAGGGCG6400.033.43755