##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553788_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1518396
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.578315538239035	31.0	31.0	34.0	30.0	34.0
2	31.746294115632548	31.0	31.0	34.0	30.0	34.0
3	31.634235074381124	31.0	31.0	34.0	30.0	34.0
4	35.46194997879341	37.0	35.0	37.0	33.0	37.0
5	33.579395625383626	37.0	35.0	37.0	30.0	37.0
6	34.412190890913834	37.0	35.0	37.0	30.0	37.0
7	35.44692030274052	37.0	35.0	37.0	32.0	37.0
8	35.557708265827884	37.0	35.0	37.0	35.0	37.0
9	37.28028985850858	39.0	37.0	39.0	34.0	39.0
10	36.60985803439946	38.0	35.0	39.0	32.0	39.0
11	36.37664943795953	38.0	35.0	39.0	32.0	39.0
12	36.67710663094476	39.0	35.0	39.0	32.0	39.0
13	33.07536834923169	38.0	35.0	39.0	10.0	39.0
14	35.34542701640415	39.0	35.0	41.0	17.0	41.0
15	36.74317832765629	39.0	35.0	41.0	30.0	41.0
16	37.341197553207465	39.0	36.0	41.0	32.0	41.0
17	37.517155603676514	39.0	36.0	41.0	32.0	41.0
18	37.60444376829233	39.0	36.0	40.0	33.0	41.0
19	37.597456131338596	39.0	37.0	41.0	32.0	41.0
20	37.57958925076199	39.0	36.0	41.0	32.0	41.0
21	37.49305714714738	39.0	36.0	41.0	32.0	41.0
22	37.50556310738437	39.0	36.0	41.0	32.0	41.0
23	37.38143870242018	39.0	36.0	40.0	32.0	41.0
24	37.37351784382994	39.0	36.0	41.0	32.0	41.0
25	37.17673913787971	39.0	35.0	40.0	32.0	41.0
26	37.15307469197759	39.0	35.0	40.0	32.0	41.0
27	36.77428417883082	39.0	35.0	40.0	31.0	41.0
28	36.8118264273615	39.0	35.0	40.0	31.0	41.0
29	36.88104552435596	39.0	35.0	40.0	31.0	41.0
30	36.418435638660796	39.0	35.0	40.0	30.0	41.0
31	36.51646606023725	39.0	35.0	40.0	30.0	41.0
32	36.468121623081196	39.0	35.0	40.0	30.0	41.0
33	36.403846559132134	39.0	35.0	40.0	30.0	41.0
34	36.35550343915553	39.0	35.0	40.0	30.0	41.0
35	36.295180572130064	39.0	35.0	40.0	30.0	41.0
36	36.17930039330978	39.0	35.0	40.0	30.0	41.0
37	36.19449010666519	39.0	35.0	40.0	30.0	41.0
38	36.07154787025256	39.0	35.0	40.0	29.0	41.0
39	36.020572367155864	39.0	35.0	40.0	29.0	41.0
40	35.938546993011045	38.0	35.0	40.0	29.0	41.0
41	35.80844917926549	38.0	35.0	40.0	28.0	41.0
42	35.72752430854665	38.0	35.0	40.0	28.0	41.0
43	35.64400920444996	38.0	35.0	40.0	28.0	41.0
44	35.564383072663524	38.0	35.0	40.0	27.0	41.0
45	35.510690228372574	38.0	34.0	40.0	27.0	41.0
46	34.39340000895682	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	14.0
10	21.0
11	31.0
12	18.0
13	13.0
14	26.0
15	32.0
16	82.0
17	163.0
18	302.0
19	546.0
20	1077.0
21	1856.0
22	3088.0
23	4594.0
24	6861.0
25	9720.0
26	13937.0
27	19283.0
28	24443.0
29	29538.0
30	36263.0
31	45346.0
32	59161.0
33	77911.0
34	106963.0
35	134575.0
36	168487.0
37	228032.0
38	289744.0
39	256268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.246265137684766	22.829024839369964	21.511779535773275	23.41293048717199
2	27.043208754501464	26.19389144860761	23.318554579964648	23.444345216926283
3	26.771408776103208	26.916825386789746	25.296431234012733	21.015334603094317
4	23.782004167555762	27.31948714301144	24.657533344397642	24.240975345035157
5	20.540886567140586	34.39320177345041	21.846277255735657	23.21963440367335
6	19.806493167790222	37.64116870697763	23.744003540578348	18.808334584653803
7	82.42401850373685	4.340369705926517	9.08985534735339	4.145756442983253
8	81.81870868995966	4.133572533120477	9.08761614229753	4.960102634622325
9	74.20277714114104	7.12093551352875	12.465852122898111	6.210435222432094
10	29.863224086470197	34.22756645828888	18.612996873015998	17.296212582224925
11	22.75651411094339	26.307037162900848	31.255153464577095	19.681295261578665
12	20.434260891098237	23.856029652343658	34.03058227234529	21.679127184212817
13	19.853582332935545	30.067123464498064	32.52023846216666	17.55905574039974
14	19.56044404753437	27.667222516392297	31.587872992289235	21.184460443784097
15	18.99603265551279	26.268114510312195	34.20576713848034	20.530085695694666
16	20.983854014367793	27.02266075516532	31.508249494861683	20.485235735605205
17	20.654229858350522	26.661753587338215	30.526687372727533	22.157329181583723
18	22.147911348554658	24.683547638428973	31.358091038174496	21.810449974841873
19	22.19473707781106	27.34536971909831	28.928487693592448	21.53140550949818
20	23.763234360469866	26.3750694812157	28.38850998026865	21.47318617804578
21	24.29234534337551	27.492630381007327	29.11486858500681	19.100155690610354
22	22.78503104591951	24.297087189376157	29.134955571537336	23.782926193167
23	20.374460944312286	27.554340237987983	29.34280648789907	22.72839232980066
24	20.497222068551288	25.828901024502173	31.021090677267328	22.652786229679215
25	21.664901646210872	27.52839180292888	28.199099576131655	22.607606974728593
26	20.68854238288299	26.848990645391584	29.32805407811928	23.13441289360615
27	21.6244642372609	25.82132724269558	28.604593268159295	23.949615251884225
28	20.444205595905153	26.895816374647985	29.62316813268739	23.036809896759475
29	21.66648226154442	25.150619469492806	28.52990919364909	24.652989075313688
30	21.86254442187677	24.835813582227562	29.226104389105345	24.075537606790324
31	21.686766824991636	24.93117737401837	28.709506610923636	24.67254919006636
32	21.782130616782446	24.835089133533018	29.100050316254784	24.28272993342975
33	21.25881522343315	24.33344134204779	28.722217392564257	25.685526041954798
34	20.317295356415585	24.167871885858496	32.31363886627731	23.201193891448607
35	21.387832950034117	23.61926664717241	30.459774656940613	24.53312574585286
36	20.183074771008354	25.1830879428028	31.103480251528588	23.53035703466026
37	20.956456681919605	24.375591084275776	31.712939180556326	22.955013053248297
38	20.4542161596843	25.643639735615743	31.01898319015593	22.883160914544032
39	21.563808123835944	23.459953793345083	31.441336779074756	23.534901303744217
40	22.0623605436263	23.39883666711451	32.08444964291265	22.45435314634654
41	19.663381621131773	24.66036528020358	30.952202192313464	24.724050906351174
42	21.210869891648816	25.11571421421026	30.010616466323675	23.662799427817248
43	21.13164154805466	23.92840866282577	31.257919541410807	23.682030247708767
44	22.112742657383187	24.518439195045296	29.970837647096015	23.3979805004755
45	21.93314524010864	23.167737533555147	30.348011981064225	24.551105245271984
46	22.616893089813196	23.696848516460793	29.568834480596628	24.117423913129382
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3866.0
1	3350.0
2	2834.0
3	11765.0
4	20696.0
5	13812.0
6	6928.0
7	6928.0
8	6369.0
9	5810.0
10	5571.0
11	5332.0
12	5201.5
13	5071.0
14	4950.5
15	4830.0
16	4702.5
17	4575.0
18	4574.0
19	4573.0
20	4573.0
21	4881.0
22	5189.0
23	6459.5
24	7730.0
25	9654.5
26	11579.0
27	14772.5
28	17966.0
29	21484.5
30	25003.0
31	25003.0
32	28196.0
33	31389.0
34	36288.5
35	41188.0
36	46711.0
37	52234.0
38	58003.0
39	63772.0
40	72030.0
41	80288.0
42	89718.0
43	99148.0
44	99148.0
45	112517.5
46	125887.0
47	142309.0
48	158731.0
49	159498.5
50	160266.0
51	147332.5
52	134399.0
53	118535.5
54	102672.0
55	91963.0
56	81254.0
57	81254.0
58	74134.0
59	67014.0
60	60741.5
61	54469.0
62	48099.0
63	41729.0
64	35499.0
65	29269.0
66	25529.5
67	21790.0
68	18591.5
69	15393.0
70	15393.0
71	12252.0
72	9111.0
73	7840.5
74	6570.0
75	5442.0
76	4314.0
77	3416.0
78	2518.0
79	2161.0
80	1804.0
81	1804.0
82	1248.0
83	692.0
84	503.0
85	314.0
86	219.5
87	125.0
88	86.0
89	47.0
90	31.0
91	15.0
92	11.5
93	8.0
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1518396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.0041243914816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.07946405051192	24.449278463773847
2	9.406776768660462	6.209248611915384
3	3.495034832731589	3.4605169311610333
4	1.990233768946241	2.6274369151371593
5	1.338784695541653	2.2092708312534266
6	1.0139592487669533	2.0078902304518658
7	0.8254340768377469	1.9069910264244545
8	0.6589871625508119	1.73994354281732
9	0.5729864983680324	1.701982590010021
>10	6.383961511902273	44.39749027661798
>50	0.19133796351339466	3.9170095934066147
>100	0.03790038756753424	2.290008900513627
>500	0.002355390629780365	0.5609121577729593
>1k	0.002569517050669489	1.7458452747414877
>5k	0.0	0.0
>10k+	2.1412642088912407E-4	0.7761746540028237
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10983	0.7233290920155216	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3491	0.2299136720591993	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	3196	0.21048527525098856	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	2310	0.152134225854125	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	2275	0.14982916182603223	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	2185	0.14390185432522215	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTC	2121	0.1396868801024239	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	2045	0.13468159821285092	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	1804	0.11880958590512619	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	1643	0.10820629137589931	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3171794446244589E-4	0.0	0.0	0.16715007152284383	0.0
2	1.3171794446244589E-4	0.0	0.0	0.686513926538268	0.0
3	1.3171794446244589E-4	0.0	0.0	1.0295733128907083	0.0
4	1.3171794446244589E-4	0.0	0.0	1.4586445169771258	0.0
5	1.3171794446244589E-4	0.0	0.0	2.505077726759027	0.0
6	1.3171794446244589E-4	0.0	0.0	3.8872599769757032	0.0
7	1.3171794446244589E-4	0.0	0.0	4.9297416484237315	0.0
8	1.3171794446244589E-4	0.0	0.0	6.6900202582198585	0.0
9	1.3171794446244589E-4	0.0	0.0	7.44621297737876	0.0
10	1.3171794446244589E-4	0.0	0.0	8.663879514961842	0.0
11	1.3171794446244589E-4	0.0	0.0	9.73547085213607	0.0
12	1.3171794446244589E-4	0.0	0.0	10.76036817799836	0.0
13	1.3171794446244589E-4	0.0	0.0	11.257800995260789	0.0
14	1.3171794446244589E-4	0.0	0.0	11.490019731348081	0.0
15	1.3171794446244589E-4	0.0	0.0	11.798042144473511	0.0
16	1.3171794446244589E-4	0.0	0.0	12.398149099444414	0.0
17	1.3171794446244589E-4	0.0	0.0	13.114760576292351	0.0
18	1.3171794446244589E-4	0.0	0.0	13.967700125658919	0.0
19	1.3171794446244589E-4	0.0	0.0	14.39209534271692	0.0
20	1.9757691669366884E-4	0.0	0.0	14.829991649082322	0.0
21	1.9757691669366884E-4	0.0	0.0	15.332495607206551	0.0
22	1.9757691669366884E-4	0.0	0.0	15.826833052774111	0.0
23	1.9757691669366884E-4	0.0	0.0	16.352980381929353	0.0
24	2.6343588892489177E-4	0.0	0.0	16.766311291652507	0.0
25	2.6343588892489177E-4	0.0	0.0	17.13439708745281	0.0
26	2.6343588892489177E-4	0.0	0.0	17.48107871727797	0.0
27	2.6343588892489177E-4	0.0	0.0	17.86562925613608	0.0
28	2.6343588892489177E-4	0.0	0.0	18.2499822180775	0.0
29	2.6343588892489177E-4	0.0	0.0	18.649877897465483	0.0
30	2.6343588892489177E-4	0.0	0.0	19.151328112034015	0.0
31	2.6343588892489177E-4	0.0	0.0	19.55596563742265	0.0
32	2.6343588892489177E-4	0.0	0.0	19.991359302843264	0.0
33	2.6343588892489177E-4	0.0	0.0	20.40429505873303	0.0
34	3.2929486115611473E-4	0.0	0.0	20.811105930205297	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATC	20	0.0012558961	40.0	1
ACGCTAG	35	3.0572483E-7	40.0	1
TATAGCG	60	0.0	40.0	1
TACGTAG	60	0.0	40.0	1
CGTTTTT	4980	0.0	38.07229	1
GCGAAGT	50	3.426976E-9	36.000004	9
GCTACGA	100	0.0	36.000004	10
CCGCACG	45	5.4444172E-8	35.555557	1
AACGGGA	1045	0.0	35.4067	4
GCGATCG	85	0.0	35.29412	9
CGTAAGG	335	0.0	35.22388	2
GCGTTAG	80	0.0	35.0	1
CCGTAAG	70	1.8189894E-12	34.285713	1
TCGATAG	100	0.0	34.000004	1
TGCACGG	390	0.0	33.846153	2
GCGATCA	285	0.0	33.68421	9
CGCACGG	410	0.0	33.65854	2
TGGGCGA	1245	0.0	33.5743	6
ACGGGAA	1205	0.0	33.52697	5
TAGGGCG	640	0.0	33.4375	5
>>END_MODULE