FastQCFastQC Report
Sat 18 Jun 2016
SRR3553786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335364
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG118930.8906185878906425No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTA86500.647763456256122No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCG78330.5865816361681159No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT45120.3378854005349852No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39830.2982707336726166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36570.27385791439637436No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT33290.24929532322273176No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGT29450.22053911892188197No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC23320.17463403236870248No Hit
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT20970.157035834424172No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATG18340.13734082991603788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG304.8681068E-640.0000041
CGTAATC200.001255841740.022
TATAGCG401.9286745E-840.01
ACCGTAA200.001255841740.020
CGCCGTT200.001255841740.026
CGATCTA200.001255841740.010
TAGTCCG200.001255841740.01
CCGTAAG650.036.9230771
CGAATAT650.036.92307714
CGTACGG650.036.9230772
GTTCACG650.036.9230771
GCTCGAG601.4551915E-1136.6666681
CGTAAGG2550.036.078432
CGTTTTT22150.036.027091
GTCAACG455.4436896E-835.5555531
CGGTCTA750.034.66666831
AAGGGAT14150.034.6289755
CGACGGT701.8189894E-1234.28571328
GCGAATT351.401237E-534.2857139
TCGACAG351.401237E-534.2857131
Produced by FastQC (version 0.11.5)