##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553786_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1335364
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.602353365823852	31.0	31.0	34.0	30.0	34.0
2	31.756959151212705	31.0	31.0	34.0	30.0	34.0
3	31.714472608217683	31.0	31.0	34.0	30.0	34.0
4	35.51616712746487	37.0	35.0	37.0	33.0	37.0
5	33.6316802010538	37.0	35.0	37.0	30.0	37.0
6	34.421512037167396	37.0	35.0	37.0	30.0	37.0
7	35.39259183263889	37.0	35.0	37.0	32.0	37.0
8	35.39975092933462	37.0	35.0	37.0	33.0	37.0
9	37.19982042349502	39.0	37.0	39.0	34.0	39.0
10	36.56497554224916	38.0	35.0	39.0	32.0	39.0
11	36.335492794474014	38.0	35.0	39.0	32.0	39.0
12	36.650597140554936	39.0	35.0	39.0	32.0	39.0
13	33.10675815732639	38.0	35.0	39.0	10.0	39.0
14	35.37948005188098	39.0	35.0	40.0	18.0	41.0
15	36.68207994224796	39.0	35.0	40.0	30.0	41.0
16	37.31351901054694	39.0	36.0	40.0	32.0	41.0
17	37.46515481921034	39.0	36.0	40.0	32.0	41.0
18	37.55054501993464	39.0	36.0	40.0	32.0	41.0
19	37.57525214098927	39.0	36.0	41.0	32.0	41.0
20	37.56108971037111	39.0	36.0	41.0	32.0	41.0
21	37.534413088865655	39.0	36.0	41.0	32.0	41.0
22	37.581219802241186	39.0	36.0	41.0	32.0	41.0
23	37.471770992778	39.0	36.0	40.0	32.0	41.0
24	37.46116414700411	39.0	36.0	41.0	32.0	41.0
25	37.2850443774132	39.0	36.0	40.0	32.0	41.0
26	37.25692095937887	39.0	36.0	40.0	32.0	41.0
27	36.885093502595545	39.0	36.0	40.0	31.0	41.0
28	36.92458685422102	39.0	35.0	40.0	31.0	41.0
29	37.00539478374436	39.0	36.0	40.0	31.0	41.0
30	36.598758840286244	39.0	35.0	40.0	30.0	41.0
31	36.737659544513704	39.0	35.0	40.0	30.0	41.0
32	36.71532780575184	39.0	35.0	40.0	30.0	41.0
33	36.69072402730641	39.0	35.0	40.0	30.0	41.0
34	36.729978492755535	39.0	35.0	40.0	30.0	41.0
35	36.666817437043385	39.0	35.0	40.0	30.0	41.0
36	36.56266680845073	39.0	35.0	40.0	30.0	41.0
37	36.55848143277788	39.0	35.0	40.0	30.0	41.0
38	36.470748799578246	39.0	35.0	40.0	30.0	41.0
39	36.3628755904757	39.0	35.0	40.0	30.0	41.0
40	36.25145877828068	39.0	35.0	40.0	30.0	41.0
41	36.046967718165234	39.0	35.0	40.0	29.0	41.0
42	36.00872046872613	38.0	35.0	40.0	29.0	41.0
43	35.902842221296964	38.0	35.0	40.0	29.0	41.0
44	35.88302515269245	38.0	35.0	40.0	29.0	41.0
45	35.88065051925917	38.0	35.0	40.0	29.0	41.0
46	34.63012257332083	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	3.0
9	13.0
10	27.0
11	27.0
12	24.0
13	15.0
14	16.0
15	22.0
16	63.0
17	115.0
18	247.0
19	456.0
20	845.0
21	1567.0
22	2462.0
23	3721.0
24	5272.0
25	7164.0
26	10132.0
27	13489.0
28	17707.0
29	22812.0
30	29899.0
31	39871.0
32	52735.0
33	69683.0
34	94886.0
35	120257.0
36	150300.0
37	203576.0
38	258919.0
39	229037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.67559107479309	21.89897286432763	21.73954067954505	20.685895381334227
2	23.841139943865493	29.76169793404645	23.126054019727952	23.271108102360106
3	25.49087739372935	29.83598479515698	23.386207805512203	21.28693000560147
4	23.577541404441035	27.68278911218215	25.519259168286702	23.220410315090117
5	22.214018050509075	33.662881431579706	20.264661919896	23.858438598015223
6	19.160693264158688	40.45481232083537	22.82426364646643	17.560230768539515
7	80.97282838237363	4.8025856620367176	10.03411803822778	4.1904679173618575
8	79.33177770255901	6.598350711865828	8.383856386723021	5.686015198852148
9	72.37749407652146	7.481630476783859	11.297069563055466	8.843805883639218
10	31.02105493333653	30.251601810442697	19.542386944683248	19.18495631153753
11	24.59172180768689	26.419987359251863	28.529000332493617	20.459290500567636
12	21.411016022597586	22.81348006985361	33.01579194886188	22.75971195868692
13	19.817143490464023	29.667266752735582	32.78147381537917	17.734115941421216
14	20.557091549570007	27.99641146533829	29.616044763824696	21.83045222126701
15	18.56362759517255	26.901878439137196	34.67099607298085	19.863497892709404
16	20.496658588968998	26.371311492596778	30.42196734373549	22.710062574698732
17	20.434053935855694	26.879936856168058	29.425609796280266	23.260399411695985
18	20.897223528565995	26.665463499090887	29.611252063107884	22.826060909235235
19	21.642114060286183	28.330402796540866	27.41282526711818	22.61465787605477
20	23.073034768048263	26.625399516536312	28.848763333443166	21.452802381972255
21	23.566008968341215	28.628973074008286	28.436740843695052	19.368277113955447
22	21.619348731881345	25.3449246797128	27.667212834852517	25.36851375355334
23	20.37422006284429	28.275661168041072	28.43696550154115	22.913153267573485
24	21.326394900566438	25.411573174055913	29.08270703718237	24.17932488819528
25	20.601723574995283	29.262208656216583	26.962011855943395	23.174055912844736
26	19.59061349564613	26.93482825656525	29.598521451828862	23.876036795959756
27	22.228021722916	26.61162050197549	27.51684184986266	23.64351592524585
28	19.52194308068811	26.86024185165992	29.725977336516486	23.89183773113548
29	21.578910319583276	24.53241213631639	28.99621376643372	24.892463777666613
30	21.123903295281288	25.41696496236232	29.78581120952789	23.6733205328285
31	20.66485242974949	25.230573836047697	27.138218493234806	26.966355240968003
32	20.52444127593675	25.277377553985282	28.637734730006198	25.56044644007177
33	19.316006721762754	24.640023244598478	27.03068227090142	29.01328776273735
34	19.296761032946822	23.676091312930406	28.97831602469439	28.048831629428378
35	18.806857156550574	23.05393885112973	30.074346769869486	28.064857222450208
36	19.31937658945426	26.138940393780274	28.93623012152492	25.60545289524055
37	18.976324058458967	25.77424582361064	29.279806854161112	25.969623263769282
38	18.749269862000173	25.838348195697954	27.756851315446575	27.655530626855303
39	20.667548323902697	24.216992520391443	26.960589022918096	28.154870132787764
40	20.656015887802877	23.37055664223388	31.73763857644807	24.235788893515174
41	18.734292672260146	26.34390323537253	29.212634158177096	25.70916993419023
42	20.093697299013602	25.29325337510971	31.78017379530974	22.832875530566945
43	20.780700992388592	25.308979424336737	29.59732327664966	24.31299630662501
44	21.174226652807775	24.22620349208156	28.47553176512172	26.12403808998895
45	20.245416231080064	22.516182853514096	29.15092813644819	28.087472778957647
46	22.561938168169878	23.958561111427297	28.12925913833232	25.350241582070502
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4296.0
1	3796.5
2	3297.0
3	5888.0
4	8479.0
5	5869.0
6	3259.0
7	3259.0
8	3048.0
9	2837.0
10	2884.5
11	2932.0
12	2812.0
13	2692.0
14	2678.5
15	2665.0
16	2852.0
17	3039.0
18	3126.5
19	3214.0
20	3214.0
21	3672.0
22	4130.0
23	5469.5
24	6809.0
25	8426.0
26	10043.0
27	11510.0
28	12977.0
29	16605.0
30	20233.0
31	20233.0
32	23807.0
33	27381.0
34	31122.5
35	34864.0
36	38966.5
37	43069.0
38	48506.5
39	53944.0
40	62080.5
41	70217.0
42	85156.5
43	100096.0
44	100096.0
45	108007.0
46	115918.0
47	139646.5
48	163375.0
49	153495.5
50	143616.0
51	129445.0
52	115274.0
53	101356.5
54	87439.0
55	77992.5
56	68546.0
57	68546.0
58	63507.5
59	58469.0
60	53729.0
61	48989.0
62	43613.0
63	38237.0
64	32218.5
65	26200.0
66	22101.0
67	18002.0
68	15130.5
69	12259.0
70	12259.0
71	9602.5
72	6946.0
73	5767.0
74	4588.0
75	3962.5
76	3337.0
77	2612.0
78	1887.0
79	1414.0
80	941.0
81	941.0
82	708.0
83	475.0
84	377.0
85	279.0
86	178.0
87	77.0
88	49.0
89	21.0
90	14.5
91	8.0
92	5.0
93	2.0
94	2.0
95	2.5
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1335364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.27645654198867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83113582286978	24.15283903374508
2	9.900283444180891	6.390921366789488
3	3.802768828556127	3.6821970850236307
4	2.017329520750806	2.6044899442953695
5	1.2738792553437939	2.055815421242243
6	0.8952994678121109	1.7338256618941907
7	0.6683566435181093	1.510052891134317
8	0.5320705147952967	1.373868067845115
9	0.45617263051667073	1.325127247606438
>10	5.086597157589915	37.13203166834392
>50	0.47737022463791395	9.614722082483276
>100	0.05034556241791531	2.8591072032020217
>500	0.004555074662923125	1.0184732698352204
>1k	0.0031166300325263492	2.350799113933596
>5k	4.794815434655921E-4	1.2754516719870792
>10k+	2.3974077173279605E-4	0.9202782706389816
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	11893	0.8906185878906425	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTA	8650	0.647763456256122	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCG	7833	0.5865816361681159	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	4512	0.3378854005349852	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3983	0.2982707336726166	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3657	0.27385791439637436	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	3329	0.24929532322273176	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGT	2945	0.22053911892188197	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	2332	0.17463403236870248	No Hit
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	2097	0.157035834424172	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATG	1834	0.13734082991603788	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18496829328932035	0.0
2	0.0	0.0	0.0	1.0025730811973363	0.0
3	0.0	0.0	0.0	1.3319214835805069	0.0
4	0.0	0.0	0.0	1.9684520475316094	0.0
5	0.0	0.0	0.0	3.9300145877828068	0.0
6	7.48859487001297E-5	0.0	0.0	5.360036664160484	0.0
7	7.48859487001297E-5	0.0	0.0	6.471194370973008	0.0
8	7.48859487001297E-5	0.0	0.0	8.33143622263293	0.0
9	7.48859487001297E-5	0.0	0.0	9.010202461650906	0.0
10	7.48859487001297E-5	0.0	0.0	10.903169472892785	0.0
11	7.48859487001297E-5	0.0	0.0	12.135267986856018	0.0
12	7.48859487001297E-5	0.0	0.0	13.846786344397483	0.0
13	7.48859487001297E-5	0.0	0.0	14.390757875755225	0.0
14	7.48859487001297E-5	0.0	0.0	14.64192534769546	0.0
15	7.48859487001297E-5	0.0	0.0	15.057542362981179	0.0
16	7.48859487001297E-5	0.0	0.0	15.812093182083686	0.0
17	7.48859487001297E-5	0.0	0.0	16.661898927932757	0.0
18	7.48859487001297E-5	0.0	0.0	17.70678257014567	0.0
19	7.48859487001297E-5	0.0	0.0	18.231882842430977	0.0
20	7.48859487001297E-5	0.0	0.0	18.722011376673326	0.0
21	7.48859487001297E-5	0.0	0.0	19.311139135097246	0.0
22	7.48859487001297E-5	0.0	0.0	19.916067828696896	0.0
23	7.48859487001297E-5	0.0	0.0	20.506468648248717	0.0
24	1.497718974002594E-4	0.0	0.0	20.961924988242906	0.0
25	1.497718974002594E-4	0.0	0.0	21.363463445173	0.0
26	1.497718974002594E-4	0.0	0.0	21.73834250436585	0.0
27	1.497718974002594E-4	0.0	0.0	22.14033027698815	0.0
28	1.497718974002594E-4	0.0	0.0	22.55182856509536	0.0
29	2.2465784610038912E-4	0.0	0.0	22.980999937095802	0.0
30	2.2465784610038912E-4	0.0	0.0	23.5001842194338	0.0
31	2.2465784610038912E-4	0.0	0.0	23.936020440868557	0.0
32	2.2465784610038912E-4	0.0	0.0	24.355980841178884	0.0
33	2.2465784610038912E-4	0.0	0.0	24.776764986924913	0.0
34	2.2465784610038912E-4	0.0	0.0	25.1734358571895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATG	30	4.8681068E-6	40.000004	1
CGTAATC	20	0.0012558417	40.0	22
TATAGCG	40	1.9286745E-8	40.0	1
ACCGTAA	20	0.0012558417	40.0	20
CGCCGTT	20	0.0012558417	40.0	26
CGATCTA	20	0.0012558417	40.0	10
TAGTCCG	20	0.0012558417	40.0	1
CCGTAAG	65	0.0	36.923077	1
CGAATAT	65	0.0	36.923077	14
CGTACGG	65	0.0	36.923077	2
GTTCACG	65	0.0	36.923077	1
GCTCGAG	60	1.4551915E-11	36.666668	1
CGTAAGG	255	0.0	36.07843	2
CGTTTTT	2215	0.0	36.02709	1
GTCAACG	45	5.4436896E-8	35.555553	1
CGGTCTA	75	0.0	34.666668	31
AAGGGAT	1415	0.0	34.628975	5
CGACGGT	70	1.8189894E-12	34.285713	28
GCGAATT	35	1.401237E-5	34.285713	9
TCGACAG	35	1.401237E-5	34.285713	1
>>END_MODULE