FastQCFastQC Report
Sat 18 Jun 2016
SRR3553785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835175
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43370.5192923638758344No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT28550.34184452360283774No Hit
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT17800.21312898494327534No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT17070.2043883018529051No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15950.19097793875535068No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG15230.18235699104977998No Hit
ATATATATATACATATATATGTATATATACATATATATGTATATAT13800.16523483102343822No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCG11390.13637860328673632No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA11380.13625886790193673No Hit
ATATATATATATGTATATATACATATATATGTATATATACATATAT10850.1299128925075583No Hit
AAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG10110.12105247403238842No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA10040.12021432633879127No Hit
ACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT9270.11099470170922261No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC9060.10848025862843116No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9050.10836052324363157No Hit
ATATATATATGTATATATACATATATATGTATATATACATATATAT8810.10548687400844135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAT257.792823E-540.037
CAATTGA200.001255571740.031
GCGTTAC200.001255571740.036
TAACCGT200.001255571740.022
TGCGAAC257.792823E-540.035
TGTTACG200.001255571740.01
TCATCGC200.001255571740.035
GTATACG353.0550473E-740.01
TATAGCG200.001255571740.01
TACTACG257.792823E-540.01
CGCAAGT200.001255571740.029
AGTAACG451.2205419E-940.01
ACGGGAC3050.038.0327875
CGTACAC601.4551915E-1136.66666825
CTACGCA1650.036.36363615
CCGTCTT2700.036.29629540
TTCACGG1050.036.190482
ACGGGCC1500.036.05
AGTACGG1450.035.862072
CGCATCG455.439506E-835.55555321
Produced by FastQC (version 0.11.5)