##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553785_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835175
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.551625707187117	31.0	31.0	34.0	30.0	34.0
2	31.71913910258329	31.0	31.0	34.0	30.0	34.0
3	31.664275151914268	31.0	31.0	34.0	30.0	34.0
4	35.47663184362559	37.0	35.0	37.0	33.0	37.0
5	33.603517825605415	37.0	35.0	37.0	30.0	37.0
6	34.4072272278265	37.0	35.0	37.0	30.0	37.0
7	35.31165803574101	37.0	35.0	37.0	32.0	37.0
8	35.39715269254947	37.0	35.0	37.0	33.0	37.0
9	37.13872421947496	39.0	37.0	39.0	34.0	39.0
10	36.55614212590176	38.0	35.0	39.0	32.0	39.0
11	36.30868979555183	38.0	35.0	39.0	32.0	39.0
12	36.57139282186368	39.0	35.0	39.0	32.0	39.0
13	33.12917173047565	38.0	35.0	39.0	15.0	39.0
14	35.31936061304517	39.0	35.0	40.0	18.0	41.0
15	36.642143263387915	39.0	35.0	40.0	30.0	41.0
16	37.24166072978717	39.0	36.0	40.0	32.0	41.0
17	37.380495105816145	39.0	36.0	40.0	32.0	41.0
18	37.520350824677465	39.0	36.0	40.0	32.0	41.0
19	37.50447151794534	39.0	36.0	40.0	32.0	41.0
20	37.51376897057503	39.0	36.0	40.0	32.0	41.0
21	37.409042416260064	39.0	36.0	40.0	32.0	41.0
22	37.42883826742898	39.0	36.0	40.0	32.0	41.0
23	37.30830305025893	39.0	36.0	40.0	32.0	41.0
24	37.322551561050076	39.0	36.0	40.0	32.0	41.0
25	37.11057323315473	39.0	35.0	40.0	31.0	41.0
26	37.08056754572395	39.0	35.0	40.0	31.0	41.0
27	36.65796270242763	39.0	35.0	40.0	31.0	41.0
28	36.69987008710749	39.0	35.0	40.0	30.0	41.0
29	36.75867452929027	39.0	35.0	40.0	30.0	41.0
30	36.241956476187625	39.0	35.0	40.0	30.0	41.0
31	36.30777741191966	39.0	35.0	40.0	30.0	41.0
32	36.290003891400005	38.0	35.0	40.0	30.0	41.0
33	36.22803843505852	38.0	35.0	40.0	30.0	41.0
34	36.27827700781273	38.0	35.0	40.0	30.0	41.0
35	36.21316490555871	38.0	35.0	40.0	30.0	41.0
36	36.07550034423923	38.0	35.0	40.0	30.0	41.0
37	36.04656149908702	38.0	35.0	40.0	30.0	41.0
38	35.9470051186877	38.0	35.0	40.0	29.0	41.0
39	35.90540904600832	38.0	35.0	40.0	29.0	41.0
40	35.78455054329931	38.0	35.0	40.0	28.0	41.0
41	35.601931331756816	38.0	34.0	40.0	28.0	41.0
42	35.51956655790703	38.0	34.0	40.0	27.0	41.0
43	35.37129942826354	38.0	34.0	40.0	27.0	41.0
44	35.263190349327985	38.0	34.0	40.0	27.0	41.0
45	35.21528302451582	38.0	34.0	40.0	26.0	41.0
46	33.87084263777053	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	15.0
11	13.0
12	18.0
13	7.0
14	18.0
15	33.0
16	48.0
17	97.0
18	210.0
19	417.0
20	720.0
21	1210.0
22	1955.0
23	2861.0
24	4037.0
25	5663.0
26	7452.0
27	9716.0
28	12511.0
29	16194.0
30	20600.0
31	26472.0
32	34365.0
33	45612.0
34	62561.0
35	79173.0
36	95847.0
37	126213.0
38	154334.0
39	126785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.70907294878319	25.609363307091325	22.116562397102406	19.565001347023077
2	25.09198670937229	27.22962253419942	24.464453557637622	23.213937198790674
3	26.735833807285896	28.820786062801208	23.616128356332506	20.82725177358039
4	25.233513934205405	27.691561648756245	23.069895530876764	24.005028886161583
5	20.405424012931423	35.81177597509504	19.45628161762505	24.32651839434849
6	18.831741850510372	39.89966174753794	22.75307570269704	18.515520699254647
7	79.67324213488192	7.666776424102734	8.030532523123895	4.62944891789146
8	78.13787529559673	6.176669560271799	9.311341934325142	6.374113209806328
9	71.72305205495854	9.114975903253809	11.572065734726255	7.589906307061395
10	32.42781452988894	26.83497470589996	20.077947735504534	20.659263028706558
11	26.03897386775227	28.071362289340556	24.766426198102195	21.12323764480498
12	21.703595054928606	23.902415661388332	31.708564073397795	22.68542521028527
13	20.968898733798305	30.348609572844016	30.037417307749877	18.645074385607806
14	20.43811177298171	27.48226419612656	29.627922291735263	22.451701739156466
15	18.85544945670069	27.61457179633011	31.224234441883436	22.30574430508576
16	20.81455982279163	25.47657676534858	30.204328434160505	23.504534977699283
17	19.698985242613823	27.398209955997245	27.935103421438622	24.96770137995031
18	22.136917412518333	24.927230819888045	28.78486544736133	24.15098632023229
19	22.589277696291195	29.417666956027176	25.338641602059447	22.654413745622175
20	24.006585446163978	25.751130002694044	27.182446792588376	23.0598377585536
21	24.486963809979944	28.841260813601938	26.418894243721375	20.25288113269674
22	21.94749603376538	25.165504235639236	25.907743886011914	26.97925584458347
23	20.798395545843682	28.811087496632442	25.707306851857396	24.68321010566648
24	21.834106624360164	25.730535516508517	28.348908911306015	24.086448947825307
25	23.307809740473555	27.604154817852546	24.270841440416678	24.817194001257224
26	20.83527404436196	26.340347831292842	27.84721764899572	24.97716047534948
27	23.075882300116742	26.60903403478313	26.306821923548956	24.00826174155117
28	20.924596641422458	26.228275511120426	29.16382794025204	23.683299907205075
29	23.853683359774898	25.73939593498369	25.597389768611368	24.809530936630047
30	24.84712784745712	24.403149040620228	27.911994492172298	22.83772861975035
31	23.92971532912264	25.975394378423683	26.206603406471697	23.88828688598198
32	23.160415481785254	25.38797258059688	28.7942048073757	22.657407130242166
33	22.584488280899212	24.20319094800491	27.70257730415781	25.50974346693807
34	22.32047175741611	23.340018558984642	29.738557787290087	24.600951896309155
35	22.786721345825725	22.804082976621668	28.857664561319485	25.55153111623312
36	23.922052264495466	24.432963151435327	28.31466459125333	23.330319992815877
37	21.647199688687998	24.3232855389589	29.332774568204268	24.69674020414883
38	21.743107731912474	24.28257550812704	30.288861615829017	23.68545514413147
39	23.656119974855567	22.04065016313946	26.980572933816266	27.322656928188703
40	24.336935372826055	21.495494956146917	30.360343640554376	23.807226030472656
41	20.183793815667375	24.19169635106415	28.554973508546112	27.069536324722364
42	22.083754901667316	23.54141347621756	29.580986020893825	24.7938456012213
43	21.653904870236776	22.635615290208637	30.47349357918999	25.236986260364596
44	22.965246804561918	23.069536324722364	29.421737959110366	24.54347891160535
45	23.483581285359357	21.866554913640854	28.415601520639388	26.234262280360404
46	25.05115694315563	22.353398988236	28.370940222109137	24.22450384649924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4953.0
1	4204.0
2	3455.0
3	3866.0
4	4277.0
5	3277.0
6	2277.0
7	2277.0
8	4062.0
9	5847.0
10	4503.0
11	3159.0
12	2679.0
13	2199.0
14	2425.0
15	2651.0
16	2769.0
17	2887.0
18	2810.5
19	2734.0
20	2734.0
21	2605.0
22	2476.0
23	2930.0
24	3384.0
25	4346.5
26	5309.0
27	6218.0
28	7127.0
29	8832.5
30	10538.0
31	10538.0
32	12027.0
33	13516.0
34	14902.0
35	16288.0
36	18773.0
37	21258.0
38	24738.0
39	28218.0
40	32184.0
41	36150.0
42	43101.0
43	50052.0
44	50052.0
45	58212.0
46	66372.0
47	75108.5
48	83845.0
49	86242.5
50	88640.0
51	81376.0
52	74112.0
53	65300.0
54	56488.0
55	52563.5
56	48639.0
57	48639.0
58	45771.0
59	42903.0
60	39900.5
61	36898.0
62	33759.5
63	30621.0
64	27134.0
65	23647.0
66	20867.5
67	18088.0
68	15566.5
69	13045.0
70	13045.0
71	10624.0
72	8203.0
73	7286.0
74	6369.0
75	5284.0
76	4199.0
77	3132.5
78	2066.0
79	1775.0
80	1484.0
81	1484.0
82	1005.5
83	527.0
84	347.0
85	167.0
86	111.5
87	56.0
88	43.0
89	30.0
90	23.0
91	16.0
92	10.5
93	5.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	835175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.76902499627722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5893514866003	26.62937076286267
2	9.47923496461941	6.591675148608743
3	3.678022587207476	3.8364377781446675
4	1.9371272186793576	2.6940809874892615
5	1.156783863700565	2.011012353614754
6	0.8160566556518941	1.7024096555243458
7	0.6081282764813337	1.480081907214573
8	0.4335437137365869	1.2059113775908994
9	0.40273218003216477	1.2602344710909197
>10	4.401555148520905	36.06584630292222
>50	0.43378305336567	9.391564646706351
>100	0.05409448667676445	3.397366483923727
>500	0.0054779227014443745	1.2872852542799826
>1k	0.004108442026083281	2.4467228700268873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4337	0.5192923638758344	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	2855	0.34184452360283774	No Hit
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	1780	0.21312898494327534	No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	1707	0.2043883018529051	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1595	0.19097793875535068	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	1523	0.18235699104977998	No Hit
ATATATATATACATATATATGTATATATACATATATATGTATATAT	1380	0.16523483102343822	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCG	1139	0.13637860328673632	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA	1138	0.13625886790193673	No Hit
ATATATATATATGTATATATACATATATATGTATATATACATATAT	1085	0.1299128925075583	No Hit
AAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	1011	0.12105247403238842	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	1004	0.12021432633879127	No Hit
ACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	927	0.11099470170922261	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC	906	0.10848025862843116	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	905	0.10836052324363157	No Hit
ATATATATATGTATATATACATATATATGTATATATACATATATAT	881	0.10548687400844135	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.197353847995929E-4	0.0	0.0	0.24737330499595894	0.0
2	1.197353847995929E-4	0.0	0.0	1.116173257101805	0.0
3	1.197353847995929E-4	0.0	0.0	1.5091447900140689	0.0
4	1.197353847995929E-4	0.0	0.0	1.9949112461460172	0.0
5	1.197353847995929E-4	0.0	0.0	3.422875445265962	0.0
6	1.197353847995929E-4	0.0	0.0	4.863651330559463	0.0
7	1.197353847995929E-4	0.0	0.0	5.919118747567875	0.0
8	1.197353847995929E-4	0.0	0.0	7.909120842937109	0.0
9	1.197353847995929E-4	0.0	0.0	8.666686622564134	0.0
10	1.197353847995929E-4	0.0	0.0	10.156554015625467	0.0
11	1.197353847995929E-4	0.0	0.0	11.59709043014937	0.0
12	1.197353847995929E-4	0.0	0.0	13.018109976950939	0.0
13	1.197353847995929E-4	0.0	0.0	13.59104379321699	0.0
14	1.197353847995929E-4	0.0	0.0	13.865477295177657	0.0
15	1.197353847995929E-4	0.0	0.0	14.235818840362798	0.0
16	1.197353847995929E-4	0.0	0.0	15.058041727781603	0.0
17	1.197353847995929E-4	0.0	0.0	15.971862184572096	0.0
18	1.197353847995929E-4	0.0	0.0	17.168138414104828	0.0
19	1.197353847995929E-4	0.0	0.0	17.68288083335828	0.0
20	1.197353847995929E-4	0.0	0.0	18.225521597270035	0.0
21	2.394707695991858E-4	0.0	0.0	18.830424761277577	0.0
22	5.986769239979645E-4	0.0	0.0	19.424312269883558	0.0
23	5.986769239979645E-4	0.0	0.0	20.032807495435087	0.0
24	5.986769239979645E-4	0.0	0.0	20.549106474690934	0.0
25	5.986769239979645E-4	0.0	0.0	20.975005238423083	0.0
26	5.986769239979645E-4	0.0	0.0	21.348938845152215	0.0
27	5.986769239979645E-4	0.0	0.0	21.759750950399617	0.0
28	5.986769239979645E-4	0.0	0.0	22.17056305564702	0.0
29	5.986769239979645E-4	0.0	0.0	22.599574939383963	0.0
30	5.986769239979645E-4	0.0	0.0	23.137785494058132	0.0
31	5.986769239979645E-4	0.0	0.0	23.57290388241985	0.0
32	5.986769239979645E-4	0.0	0.0	24.019636603107134	0.0
33	5.986769239979645E-4	0.0	0.0	24.432723680665728	0.0
34	5.986769239979645E-4	0.0	0.0	24.83503457359236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAT	25	7.792823E-5	40.0	37
CAATTGA	20	0.0012555717	40.0	31
GCGTTAC	20	0.0012555717	40.0	36
TAACCGT	20	0.0012555717	40.0	22
TGCGAAC	25	7.792823E-5	40.0	35
TGTTACG	20	0.0012555717	40.0	1
TCATCGC	20	0.0012555717	40.0	35
GTATACG	35	3.0550473E-7	40.0	1
TATAGCG	20	0.0012555717	40.0	1
TACTACG	25	7.792823E-5	40.0	1
CGCAAGT	20	0.0012555717	40.0	29
AGTAACG	45	1.2205419E-9	40.0	1
ACGGGAC	305	0.0	38.032787	5
CGTACAC	60	1.4551915E-11	36.666668	25
CTACGCA	165	0.0	36.363636	15
CCGTCTT	270	0.0	36.296295	40
TTCACGG	105	0.0	36.19048	2
ACGGGCC	150	0.0	36.0	5
AGTACGG	145	0.0	35.86207	2
CGCATCG	45	5.439506E-8	35.555553	21
>>END_MODULE