FastQCFastQC Report
Sat 18 Jun 2016
SRR3553779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086360
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG120071.1052505615081558No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTA72630.6685629073235392No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCG64750.5960270996723002No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59290.5457675172134467No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45690.4205788136529327No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT33100.30468721234213336No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT30840.2838837954269303No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC20370.18750690378879928No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT16170.1488456865127582No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATG16170.1488456865127582No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC13710.126201259251077No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT12760.11745646010530579No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC12400.11414264148164513No Hit
CGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC12000.11046062078868885No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAT257.79437E-540.037
GTTAACG200.001255738540.01
GCTCGAG555.456968E-1240.01
TATAACG507.8216544E-1140.01
ATAACGC1250.036.811
TCGTGTA1850.036.75675617
GCGAGAC2300.036.5217421
CGTTTTT25050.036.2475051
ACGGGCT5750.036.1739125
GCAACCG455.4420525E-835.5555531
TCGATAG455.4420525E-835.5555531
CGTAAGG1300.035.3846172
TCTACGG1700.035.2941172
CCCTCGT2000.035.014
CGTACGG1150.034.7826082
GCACTTA8750.034.28571336
TAGTACG351.4009969E-534.2857131
GTCTTAC4250.033.88235531
TAGCGAG1300.033.8461531
TAGGGCG4350.033.5632175
Produced by FastQC (version 0.11.5)