##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553779_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086360
Sequences flagged as poor quality	0
Sequence length	46
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.562640377038917	31.0	31.0	34.0	30.0	34.0
2	31.714530174159577	31.0	31.0	34.0	30.0	34.0
3	31.6412837365146	31.0	31.0	34.0	30.0	34.0
4	35.462826319083916	37.0	35.0	37.0	33.0	37.0
5	33.5729877756913	37.0	35.0	37.0	30.0	37.0
6	34.36739386575353	37.0	35.0	37.0	30.0	37.0
7	35.26741779888803	37.0	35.0	37.0	32.0	37.0
8	35.24663003056077	37.0	35.0	37.0	33.0	37.0
9	37.0754298759159	39.0	37.0	39.0	33.0	39.0
10	36.545699399830625	38.0	35.0	39.0	32.0	39.0
11	36.28411300121507	38.0	35.0	39.0	32.0	39.0
12	36.51895688353768	39.0	35.0	39.0	32.0	39.0
13	32.93315199381421	38.0	35.0	39.0	10.0	39.0
14	35.18879469052616	39.0	34.0	40.0	17.0	41.0
15	36.52354007879524	39.0	34.0	40.0	30.0	41.0
16	37.160022460326225	39.0	36.0	40.0	32.0	41.0
17	37.27043429434073	39.0	36.0	40.0	32.0	41.0
18	37.35333867226334	39.0	36.0	40.0	32.0	41.0
19	37.33590154276667	39.0	36.0	40.0	32.0	41.0
20	37.2961200706948	39.0	36.0	40.0	32.0	41.0
21	37.270304503111305	39.0	36.0	40.0	32.0	41.0
22	37.297983173165434	39.0	36.0	40.0	32.0	41.0
23	37.17071688942892	39.0	35.0	40.0	32.0	41.0
24	37.12657590485659	39.0	35.0	40.0	32.0	41.0
25	36.91108840531684	39.0	35.0	40.0	31.0	41.0
26	36.85399867447255	39.0	35.0	40.0	31.0	41.0
27	36.456579770978315	39.0	35.0	40.0	30.0	41.0
28	36.45654387127656	39.0	35.0	40.0	30.0	41.0
29	36.501621009610076	39.0	35.0	40.0	30.0	41.0
30	36.06075334143378	38.0	35.0	40.0	30.0	41.0
31	36.184584299863765	38.0	35.0	40.0	30.0	41.0
32	36.075681173828194	38.0	35.0	40.0	29.0	41.0
33	36.051141426414816	38.0	35.0	40.0	29.0	41.0
34	36.027508376597076	38.0	35.0	40.0	29.0	41.0
35	35.904269302993484	38.0	35.0	40.0	29.0	41.0
36	35.843229684450826	38.0	35.0	40.0	29.0	41.0
37	35.760112669833205	38.0	35.0	40.0	28.0	41.0
38	35.6576429544534	38.0	35.0	40.0	28.0	41.0
39	35.540017121396225	38.0	34.0	40.0	27.0	41.0
40	35.41522147354468	38.0	34.0	40.0	27.0	41.0
41	35.2530956588976	38.0	34.0	40.0	26.0	41.0
42	35.198111123384514	38.0	34.0	40.0	26.0	41.0
43	35.120333038771676	38.0	34.0	40.0	26.0	41.0
44	35.064910342796125	38.0	34.0	40.0	26.0	41.0
45	35.00578353400346	37.0	34.0	40.0	26.0	41.0
46	33.7826052137413	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	12.0
10	22.0
11	17.0
12	16.0
13	16.0
14	22.0
15	42.0
16	85.0
17	116.0
18	336.0
19	634.0
20	1202.0
21	2066.0
22	3156.0
23	4437.0
24	6186.0
25	8342.0
26	10841.0
27	14153.0
28	18331.0
29	22518.0
30	28080.0
31	36246.0
32	47021.0
33	61835.0
34	83822.0
35	103189.0
36	122411.0
37	158860.0
38	190601.0
39	161742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.935932839942566	21.72659155344453	20.73226186531168	22.605213741301224
2	25.48381751905446	30.12528075407784	21.730273574137488	22.66062815273022
3	26.688390588755105	29.537262049412718	23.079181855002027	20.69516550683015
4	24.1259803380095	26.555653742774034	25.69829522441916	23.620070694797306
5	21.81256673662506	33.87366987002467	20.121506682867558	24.192256710482713
6	19.73710372252292	40.348503258588316	21.854449722007438	18.05994329688133
7	78.91905077506536	5.659081704039177	11.083158437350418	4.33870908354505
8	77.40500386612172	7.642586251334732	9.467211605729224	5.485198276814315
9	70.9248315475533	7.491071099819581	11.68866674030708	9.895430612320041
10	34.32839942560477	25.3372730954748	19.852811222799073	20.48151625612136
11	27.651791303067125	26.35765308001031	24.67671858315844	21.31383703376413
12	23.029750727199087	22.45876136823889	30.709249235980707	23.802238668581317
13	21.23771125593726	28.96507603372731	30.669575463014105	19.12763724732133
14	21.16710850914982	27.46980743031776	28.66756876173644	22.69551529879598
15	19.848024595898227	24.798041164991346	33.00765860304135	22.346275636069073
16	21.85242461062631	24.658308479693655	28.854247210869328	24.635019698810705
17	21.074413638204646	25.09232666887588	28.959092750101256	24.874166942818217
18	21.851596155970395	24.69144666593026	28.850012887072424	24.606944291026917
19	23.626514231009978	27.04582274752384	25.92519974962259	23.402463271843587
20	24.922401413895944	25.987241798298903	26.79967966419971	22.290677123605434
21	24.860911668323578	27.698645016384994	26.52619757723038	20.914245738061048
22	23.060771751537242	23.423542840310763	26.267259471998234	27.248425936153758
23	22.485916270849444	26.69188850841342	26.78476748039324	24.0374277403439
24	23.531794248683678	24.783128981184873	27.182609816267167	24.50246695386428
25	23.775083765970763	27.527983357266468	24.69687764645237	24.000055230310394
26	21.475569792702235	25.297323170956222	26.293309768400896	26.933797267940644
27	24.033285467064324	24.85557273831879	25.414963732096176	25.696178062520715
28	21.49453219927096	25.824404433152914	27.540410177105194	25.14065319047093
29	23.613074855480686	25.266025995066094	24.65701977245112	26.463879377002097
30	22.49944769689606	24.946702750469456	27.12406568724916	25.429783865385325
31	23.30452152141095	24.703689384734343	24.683254169888436	27.30853492396627
32	23.12198534555764	27.655841525829377	23.920247431790568	25.301925696822416
33	21.247376560256267	24.81295334879782	24.34229905372068	29.59737103722523
34	21.351945947936226	25.256728892816376	26.35286645310947	27.03845870613793
35	21.135627232225048	25.847877315070512	26.146857395338564	26.86963805736588
36	21.288062888913437	26.10865643064914	24.243528848632128	28.359751831805298
37	21.16140137707574	26.647244007511322	27.692017379137674	24.499337236275267
38	21.760374093302403	26.984609153503442	24.419437387238116	26.835579365956036
39	22.70849442173865	24.15350344268935	25.90200301925697	27.235999116315035
40	21.808608564380133	24.316709009904635	29.205511984977356	24.66917044073788
41	20.372620494127176	24.919547847858905	27.752862771088772	26.954968886925144
42	21.84745388269082	25.56353326705696	27.659339445487685	24.929673404764536
43	22.301354983615006	22.901340255532237	27.559004381604623	27.23830037924813
44	22.78406789646158	23.15401892558636	27.140450679332815	26.921462498619242
45	22.38309584299864	22.18500312971759	27.92803490555617	27.503866121727604
46	24.68923745351449	23.63829669722744	25.890404654074157	25.78206119518392
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4982.0
1	4220.0
2	3458.0
3	7495.5
4	11533.0
5	7834.5
6	4136.0
7	4136.0
8	3922.5
9	3709.0
10	3629.0
11	3549.0
12	3427.5
13	3306.0
14	3340.5
15	3375.0
16	3142.5
17	2910.0
18	3010.5
19	3111.0
20	3111.0
21	3111.0
22	3111.0
23	3537.0
24	3963.0
25	4988.5
26	6014.0
27	7242.5
28	8471.0
29	10100.0
30	11729.0
31	11729.0
32	13889.0
33	16049.0
34	18624.0
35	21199.0
36	22817.5
37	24436.0
38	28166.0
39	31896.0
40	36681.5
41	41467.0
42	52019.0
43	62571.0
44	62571.0
45	68859.5
46	75148.0
47	95416.0
48	115684.0
49	109588.5
50	103493.0
51	95678.0
52	87863.0
53	79186.5
54	70510.0
55	66638.5
56	62767.0
57	62767.0
58	61070.0
59	59373.0
60	57200.0
61	55027.0
62	50585.0
63	46143.0
64	41972.5
65	37802.0
66	34036.5
67	30271.0
68	26761.0
69	23251.0
70	23251.0
71	19462.5
72	15674.0
73	13067.5
74	10461.0
75	9088.0
76	7715.0
77	6262.0
78	4809.0
79	3787.0
80	2765.0
81	2765.0
82	2097.0
83	1429.0
84	1089.0
85	749.0
86	537.5
87	326.0
88	198.0
89	70.0
90	51.5
91	33.0
92	22.0
93	11.0
94	11.0
95	9.5
96	8.0
97	5.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1086360.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.32546766069022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58394025092198	25.944540967505645
2	9.394671955337962	6.449530167714931
3	3.8170370907151545	3.930647496509944
4	2.0276441441969157	2.7839933399607655
5	1.2704779863559785	2.180487551714049
6	0.8758915248501234	1.8039231726309335
7	0.6735681215549727	1.6184378541594953
8	0.5047333718840078	1.386016722710051
9	0.4164523426176394	1.2865429276866418
>10	5.185161031302956	39.78416114615759
>50	0.20028560500217849	4.266703234298509
>100	0.041647842945222495	2.634163604328066
>500	0.004509639042295057	1.012817648755753
>1k	0.0029180017332497425	2.033921407916682
>5k	7.958186545226571E-4	1.790801465259318
>10k+	2.652728848408857E-4	1.0933112926917492
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	12007	1.1052505615081558	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTA	7263	0.6685629073235392	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCG	6475	0.5960270996723002	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5929	0.5457675172134467	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4569	0.4205788136529327	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	3310	0.30468721234213336	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT	3084	0.2838837954269303	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	2037	0.18750690378879928	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	1617	0.1488456865127582	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATG	1617	0.1488456865127582	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC	1371	0.126201259251077	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	1276	0.11745646010530579	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC	1240	0.11414264148164513	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC	1200	0.11046062078868885	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8410103464781472E-4	0.0	0.0	0.13733937184726977	0.0
2	1.8410103464781472E-4	0.0	0.0	0.7387054015243566	0.0
3	1.8410103464781472E-4	0.0	0.0	1.0193674288449501	0.0
4	1.8410103464781472E-4	0.0	0.0	1.6040723148864096	0.0
5	1.8410103464781472E-4	0.0	0.0	3.459902794653706	0.0
6	1.8410103464781472E-4	0.0	0.0	4.669354541772525	0.0
7	1.8410103464781472E-4	0.0	0.0	5.652269965757208	0.0
8	1.8410103464781472E-4	0.0	0.0	7.191722817482234	0.0
9	1.8410103464781472E-4	0.0	0.0	7.809381788725653	0.0
10	1.8410103464781472E-4	0.0	0.0	9.521337309915682	0.0
11	1.8410103464781472E-4	0.0	0.0	10.434938694355463	0.0
12	1.8410103464781472E-4	0.0	0.0	11.968132110902463	0.0
13	1.8410103464781472E-4	0.0	0.0	12.45618395375382	0.0
14	1.8410103464781472E-4	0.0	0.0	12.694318642070769	0.0
15	1.8410103464781472E-4	0.0	0.0	13.063809418608933	0.0
16	1.8410103464781472E-4	0.0	0.0	13.61878198755477	0.0
17	1.8410103464781472E-4	0.0	0.0	14.181578850473139	0.0
18	2.761515519717221E-4	0.0	0.0	14.942560477189883	0.0
19	2.761515519717221E-4	0.0	0.0	15.314812769247764	0.0
20	2.761515519717221E-4	0.0	0.0	15.66129091645495	0.0
21	2.761515519717221E-4	0.0	0.0	16.035568319893958	0.0
22	2.761515519717221E-4	0.0	0.0	16.42365330093155	0.0
23	2.761515519717221E-4	0.0	0.0	16.828031223535476	0.0
24	3.6820206929562944E-4	0.0	0.0	17.154350307448727	0.0
25	3.6820206929562944E-4	0.0	0.0	17.43749769873707	0.0
26	3.6820206929562944E-4	0.0	0.0	17.704260097941752	0.0
27	3.6820206929562944E-4	0.0	0.0	17.998361500791635	0.0
28	4.602525866195368E-4	0.0	0.0	18.280588386906736	0.0
29	4.602525866195368E-4	0.0	0.0	18.58601200338746	0.0
30	4.602525866195368E-4	0.0	0.0	18.945193121985344	0.0
31	4.602525866195368E-4	0.0	0.0	19.244541404322693	0.0
32	4.602525866195368E-4	0.0	0.0	19.54342943407342	0.0
33	4.602525866195368E-4	0.0	0.0	19.848024595898227	0.0
34	4.602525866195368E-4	0.0	0.0	20.140561139953608	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAT	25	7.79437E-5	40.0	37
GTTAACG	20	0.0012557385	40.0	1
GCTCGAG	55	5.456968E-12	40.0	1
TATAACG	50	7.8216544E-11	40.0	1
ATAACGC	125	0.0	36.8	11
TCGTGTA	185	0.0	36.756756	17
GCGAGAC	230	0.0	36.52174	21
CGTTTTT	2505	0.0	36.247505	1
ACGGGCT	575	0.0	36.173912	5
GCAACCG	45	5.4420525E-8	35.555553	1
TCGATAG	45	5.4420525E-8	35.555553	1
CGTAAGG	130	0.0	35.384617	2
TCTACGG	170	0.0	35.294117	2
CCCTCGT	200	0.0	35.0	14
CGTACGG	115	0.0	34.782608	2
GCACTTA	875	0.0	34.285713	36
TAGTACG	35	1.4009969E-5	34.285713	1
GTCTTAC	425	0.0	33.882355	31
TAGCGAG	130	0.0	33.846153	1
TAGGGCG	435	0.0	33.563217	5
>>END_MODULE