##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553778_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519797
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56901828983238	31.0	31.0	34.0	30.0	34.0
2	31.732283949310983	31.0	31.0	34.0	30.0	34.0
3	31.677281708051414	31.0	31.0	34.0	30.0	34.0
4	35.48014320975304	37.0	35.0	37.0	33.0	37.0
5	33.62150224029765	37.0	35.0	37.0	30.0	37.0
6	34.42985627081342	37.0	35.0	37.0	30.0	37.0
7	35.32175253031472	37.0	35.0	37.0	32.0	37.0
8	35.41593737555238	37.0	35.0	37.0	33.0	37.0
9	37.14553181338099	39.0	37.0	39.0	34.0	39.0
10	36.59357402986166	38.0	35.0	39.0	32.0	39.0
11	36.36156230220644	38.0	35.0	39.0	32.0	39.0
12	36.57831037885944	39.0	35.0	39.0	32.0	39.0
13	33.179522005706076	38.0	35.0	39.0	15.0	39.0
14	35.36158923579782	39.0	35.0	40.0	18.0	41.0
15	36.65224501103315	39.0	35.0	40.0	30.0	41.0
16	37.255278502954035	39.0	36.0	40.0	32.0	41.0
17	37.39706847096078	39.0	36.0	40.0	32.0	41.0
18	37.49951230961317	39.0	36.0	40.0	32.0	41.0
19	37.44685713845982	39.0	36.0	40.0	32.0	41.0
20	37.460064217377166	39.0	36.0	40.0	32.0	41.0
21	37.3337668358994	39.0	36.0	40.0	32.0	41.0
22	37.331542890782366	39.0	36.0	40.0	32.0	41.0
23	37.204989640186454	39.0	35.0	40.0	32.0	41.0
24	37.199389377006796	39.0	35.0	40.0	32.0	41.0
25	36.98794144637233	39.0	35.0	40.0	31.0	41.0
26	36.96269120445097	39.0	35.0	40.0	31.0	41.0
27	36.55658458975331	39.0	35.0	40.0	31.0	41.0
28	36.54912975642414	39.0	35.0	40.0	30.0	41.0
29	36.59968025979373	39.0	35.0	40.0	30.0	41.0
30	36.14154564185634	38.0	35.0	40.0	30.0	41.0
31	36.186203460196964	38.0	35.0	40.0	30.0	41.0
32	36.086980109542765	38.0	35.0	40.0	29.0	41.0
33	36.046839439242625	38.0	35.0	40.0	30.0	41.0
34	35.99079833088687	38.0	35.0	40.0	29.0	41.0
35	35.936277046616276	38.0	35.0	40.0	29.0	41.0
36	35.77713222661924	38.0	35.0	40.0	29.0	41.0
37	35.721745219768486	38.0	35.0	40.0	28.0	41.0
38	35.56619026273719	38.0	34.0	40.0	28.0	41.0
39	35.51184981829445	38.0	34.0	40.0	27.0	41.0
40	35.42897515760961	38.0	34.0	40.0	27.0	41.0
41	35.21820826207154	38.0	34.0	40.0	27.0	41.0
42	35.11268437486172	38.0	34.0	40.0	26.0	41.0
43	34.97861280461411	38.0	34.0	40.0	26.0	41.0
44	34.88696356462234	37.0	34.0	40.0	26.0	41.0
45	34.83424875480235	37.0	34.0	40.0	26.0	41.0
46	33.5657881826944	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	12.0
10	6.0
11	10.0
12	13.0
13	17.0
14	12.0
15	20.0
16	50.0
17	96.0
18	181.0
19	328.0
20	559.0
21	920.0
22	1450.0
23	2144.0
24	2885.0
25	4090.0
26	5404.0
27	6568.0
28	8472.0
29	10333.0
30	12936.0
31	16578.0
32	21509.0
33	28699.0
34	39698.0
35	49923.0
36	60104.0
37	77841.0
38	93984.0
39	74952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.89611136655271	25.792569022137492	21.329865312804806	19.981454298504993
2	25.377599332046934	28.5142084313684	23.580359255632487	22.527832980952176
3	27.428015167459602	28.188696741227826	23.286398343968894	21.096889747343674
4	24.831616958158666	29.63387630171009	21.77619339857675	23.75831334155449
5	19.724238500799352	36.301863996906484	19.81812130504793	24.155776197246233
6	18.578406570257236	40.15086658830274	22.02013478338659	19.25059205805343
7	80.27980153790807	7.985809844997181	7.289191742160882	4.445196874933869
8	79.43004672978104	6.526009192049973	8.252067634095619	5.79187644407336
9	72.45347703045611	9.68512707845563	11.131653318507032	6.72974257258122
10	32.783375048336175	28.888585351589178	18.54762532296262	19.780414277112026
11	26.848558187138437	28.81297891292178	23.307945217075126	21.03051768286466
12	21.896047880230167	24.8152644205334	30.29259499381489	22.99609270542154
13	21.890084013566835	30.240459256209633	28.99785877948507	18.87159795073846
14	21.088424904337653	27.9911196101555	28.55364690446463	22.366808581042214
15	19.396802982702862	27.543637227610006	30.32934010777284	22.730219681914285
16	21.25618270209332	25.61807782653613	30.24623074007738	22.87950873129318
17	20.549175928295085	27.11116070312064	26.980340402118518	25.359322966465754
18	22.892205995802207	25.65520770608526	27.689078621077073	23.76350767703546
19	23.5178348470653	29.044992564404946	25.20964915149568	22.227523437034073
20	25.26659445899072	26.55459727547485	24.63673318622462	23.542075079309807
21	25.74062566732782	29.50170932113883	24.668091581906012	20.089573429627336
22	23.12114152255592	25.473598347047023	24.611531040002156	26.7937290903949
23	21.96318947589155	29.06884803105828	24.49610136264734	24.471861130402832
24	21.919326198496723	26.822009361346833	26.995346260174646	24.2633181799818
25	23.767547715742875	28.34260297770091	23.78505455014169	24.104794756414524
26	22.295626946673412	27.00188727522475	25.308533908429638	25.3939518696722
27	24.296408020823517	26.644824806607193	24.845660902236837	24.213106270332457
28	22.19020117468935	28.010165506919048	26.167523090745043	23.632110227646564
29	24.867015392547476	27.360873571798223	22.66711812496032	25.104992910693984
30	25.559401073880768	25.96725260053444	25.489758501876693	22.983587823708103
31	26.33489612290952	26.801809167809743	23.14961417630344	23.713680532977296
32	25.802765310303833	26.426277950815415	24.5126462830682	23.25831045581256
33	24.32853594768727	26.00996158115572	23.295247952566097	26.36625451859091
34	23.755619982416214	25.478407916936803	27.431285674984657	23.334686425662326
35	25.73331512109535	25.041506588148838	24.562281044330767	24.662897246425047
36	25.356052458940702	28.199085412189756	24.711185328118475	21.733676800751063
37	25.21522825256783	27.57903566199882	24.593639440012158	22.612096645421193
38	22.920293883958546	28.77565665057705	25.625388372768597	22.678661092695805
39	24.149235182196126	25.36240109119522	25.269864966515776	25.218498760092885
40	25.590951852357747	24.5014880809239	28.46361175612787	21.44394831059048
41	22.615559535741838	27.10250347731903	25.790452811385983	24.49148417555315
42	24.288135560613085	27.635211438311497	25.597108101816673	22.479544899258748
43	24.359509577777477	24.145195143488706	27.703892096337608	23.791403182396202
44	23.610178588949147	24.14827326821817	27.54132863406291	24.70021950876977
45	25.065362054802165	22.442222636914025	26.804887292539203	25.68752801574461
46	25.775254570534265	23.54399890726572	26.646171486176335	24.03457503602368
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4725.0
1	3837.5
2	2950.0
3	3308.0
4	3666.0
5	2793.5
6	1921.0
7	1921.0
8	2446.0
9	2971.0
10	2589.0
11	2207.0
12	2026.0
13	1845.0
14	1868.5
15	1892.0
16	1833.5
17	1775.0
18	1779.5
19	1784.0
20	1784.0
21	1860.0
22	1936.0
23	1941.0
24	1946.0
25	2633.0
26	3320.0
27	3694.5
28	4069.0
29	4924.5
30	5780.0
31	5780.0
32	6815.5
33	7851.0
34	8465.0
35	9079.0
36	10372.5
37	11666.0
38	13043.0
39	14420.0
40	17040.0
41	19660.0
42	23014.0
43	26368.0
44	26368.0
45	31556.5
46	36745.0
47	42399.0
48	48053.0
49	50403.5
50	52754.0
51	49036.0
52	45318.0
53	41551.5
54	37785.0
55	35561.0
56	33337.0
57	33337.0
58	30371.5
59	27406.0
60	25615.0
61	23824.0
62	22308.5
63	20793.0
64	18796.0
65	16799.0
66	15109.0
67	13419.0
68	11847.5
69	10276.0
70	10276.0
71	8996.5
72	7717.0
73	6544.5
74	5372.0
75	4406.0
76	3440.0
77	2819.0
78	2198.0
79	1794.0
80	1390.0
81	1390.0
82	1097.5
83	805.0
84	559.0
85	313.0
86	232.0
87	151.0
88	96.5
89	42.0
90	29.5
91	17.0
92	12.0
93	7.0
94	7.0
95	6.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	519797.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.56155357120051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33291154697392	28.671827463173706
2	9.444372626392727	7.094906167052602
3	3.6201545388197447	4.079358859377076
4	1.9660988326884838	2.9539890652121312
5	1.179465341280068	2.2151275300932785
6	0.8353901386958521	1.8827130868486124
7	0.6542626788276601	1.720258586229562
8	0.5000744694385042	1.502685917872324
9	0.42659306358757987	1.4421148389942686
>10	4.842608633281391	39.19404638708141
>50	0.14739953861514865	3.500525768171519
>100	0.04606235581722348	3.5345525165352027
>500	0.003070823721148232	0.8360583538785813
>1k	0.001535411860574116	1.3718354594797784
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4375	0.8416747307121818	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1442	0.27741599124273514	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT	1319	0.25375290738499834	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	882	0.16968162571157586	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTT	869	0.16718064936888824	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT	864	0.16621873539093146	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	639	0.12293260638287638	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	591	0.11369823219449131	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19950095902823603	0.0
2	0.0	0.0	0.0	0.8570653543594904	0.0
3	0.0	0.0	0.0	1.195274309009094	0.0
4	0.0	0.0	0.0	1.5702283776166466	0.0
5	0.0	0.0	0.0	2.625063245844051	0.0
6	0.0	0.0	0.0	3.9992535547531056	0.0
7	0.0	0.0	0.0	5.018497605796109	0.0
8	0.0	0.0	0.0	6.975800168142563	0.0
9	0.0	0.0	0.0	7.808625290257543	0.0
10	0.0	0.0	0.0	9.160883960469183	0.0
11	0.0	0.0	0.0	10.569895555380274	0.0
12	0.0	0.0	0.0	11.791718690180975	0.0
13	0.0	0.0	0.0	12.37290711566246	0.0
14	0.0	0.0	0.0	12.635124866053479	0.0
15	0.0	0.0	0.0	12.955057455121903	0.0
16	0.0	0.0	0.0	13.649174581615515	0.0
17	0.0	0.0	0.0	14.456989940303618	0.0
18	0.0	0.0	0.0	15.533371681637254	0.0
19	0.0	0.0	0.0	15.985856016868123	0.0
20	0.0	0.0	0.0	16.457963397249312	0.0
21	0.0	0.0	0.0	16.98528463996522	0.0
22	0.0	0.0	0.0	17.49375236871317	0.0
23	0.0	0.0	0.0	18.03415564152929	0.0
24	0.0	0.0	0.0	18.46393880688038	0.0
25	0.0	0.0	0.0	18.815614557221377	0.0
26	0.0	0.0	0.0	19.13285378715152	0.0
27	0.0	0.0	0.0	19.503383051460474	0.0
28	0.0	0.0	0.0	19.85063399750287	0.0
29	0.0	0.0	0.0	20.228858573635478	0.0
30	0.0	0.0	0.0	20.72308997550967	0.0
31	0.0	0.0	0.0	21.072841897894754	0.0
32	0.0	0.0	0.0	21.471266667564453	0.0
33	0.0	0.0	0.0	21.848529329719103	0.0
34	0.0	0.0	0.0	22.166730473627204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACT	20	0.001255134	40.000004	35
ATCTACC	20	0.001255134	40.000004	15
CTATAAC	40	1.9248546E-8	40.000004	37
CGGCACG	20	0.001255134	40.000004	32
TAGCGCA	20	0.001255134	40.000004	21
ATTCGCC	20	0.001255134	40.000004	11
GCGCTAT	20	0.001255134	40.000004	34
GCGTAAG	20	0.001255134	40.000004	1
CCCGCGA	20	0.001255134	40.000004	36
TGTCACG	25	7.7887555E-5	40.0	1
ACTTCGG	55	5.456968E-12	40.0	12
ACGATAG	35	3.052064E-7	40.0	1
GTTCGAT	50	7.8216544E-11	40.0	39
CGTAGTG	25	7.7887555E-5	40.0	28
CGGGCTA	65	0.0	40.0	6
CGAATAT	70	0.0	40.0	14
GCTACGA	70	0.0	40.0	10
GGTTCGA	50	7.8216544E-11	40.0	38
TTCGATT	50	7.8216544E-11	40.0	40
CGTCATC	25	7.7887555E-5	40.0	38
>>END_MODULE