SRR3553777_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553777_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563439
Sequences flagged as poor quality	0
Sequence length	46
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	7956	1.4120428298360603	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA	6599	1.1712004316350129	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCG	6210	1.102160127360726	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4844	0.8597203956417643	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3046	0.5406086550629261	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	2719	0.48257220391204725	Illumina PCR Primer Index 7 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGT	1867	0.3313579642161796	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	1668	0.296039145320079	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATG	1569	0.278468476623024	No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	1125	0.1996666897392619	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	1096	0.19451972618153873	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATC	947	0.16807498238496094	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	906	0.16079824080335228	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	746	0.13240120048487947	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	708	0.12565690340924218	No Hit
GAATGATACCTGACTCTTATACACATCTGACGCAGAGCATCTCGTA	627	0.11128090174801532	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTACG	30	4.86309E-6	40.000004	1
TTACGCG	30	4.86309E-6	40.000004	1
TGCCGAT	30	4.86309E-6	40.000004	14
CTTAACG	35	3.0526826E-7	40.0	1
CGAGTAT	20	0.0012552239	40.0	29
TGCCACG	20	0.0012552239	40.0	1
TTGTCGT	20	0.0012552239	40.0	31
CACGAGT	20	0.0012552239	40.0	27
ATGTCGT	20	0.0012552239	40.0	39
TATGCGG	65	0.0	40.0	2
TCTAGCG	20	0.0012552239	40.0	1
CGTTATT	65	0.0	40.0	1
ATCCGTT	20	0.0012552239	40.0	34
CCGAGTG	20	0.0012552239	40.0	27
CGTACGG	35	3.0526826E-7	40.0	2
TCGTAAG	25	7.789588E-5	40.0	1
ATGCGGA	20	0.0012552239	40.0	12
CAGTACG	20	0.0012552239	40.0	1
ACGAGTA	20	0.0012552239	40.0	28
TTACCAG	20	0.0012552239	40.0	1