##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553777_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563439
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.543196335361948	31.0	31.0	34.0	30.0	34.0
2	31.698906181503233	31.0	31.0	34.0	30.0	34.0
3	31.624461565493338	31.0	31.0	34.0	30.0	34.0
4	35.43354293898718	37.0	35.0	37.0	33.0	37.0
5	33.5778513734406	37.0	35.0	37.0	30.0	37.0
6	34.35442168539984	36.0	35.0	37.0	30.0	37.0
7	35.19327380603757	37.0	35.0	37.0	32.0	37.0
8	35.070534698521044	37.0	35.0	37.0	32.0	37.0
9	36.94443941580189	39.0	37.0	39.0	33.0	39.0
10	36.50868328248488	38.0	35.0	39.0	32.0	39.0
11	36.24653245515486	38.0	35.0	39.0	32.0	39.0
12	36.47041649584072	38.0	35.0	39.0	32.0	39.0
13	32.91180944166094	38.0	34.0	39.0	12.0	39.0
14	35.17295217405966	39.0	34.0	40.0	18.0	41.0
15	36.435440216243464	39.0	34.0	40.0	29.0	41.0
16	37.11564162225192	39.0	36.0	40.0	32.0	41.0
17	37.209541760510014	39.0	36.0	40.0	32.0	41.0
18	37.310828678880945	39.0	36.0	40.0	32.0	41.0
19	37.27128047579241	39.0	36.0	40.0	32.0	41.0
20	37.164504054564915	39.0	35.0	40.0	31.0	41.0
21	37.19856985405696	39.0	35.0	40.0	32.0	41.0
22	37.23314147582968	39.0	35.0	40.0	32.0	41.0
23	37.0694431872838	39.0	35.0	40.0	31.0	41.0
24	37.05116259257879	39.0	35.0	40.0	31.0	41.0
25	36.83732755453563	39.0	35.0	40.0	31.0	41.0
26	36.751627061669495	39.0	35.0	40.0	31.0	41.0
27	36.32325238402028	39.0	35.0	40.0	30.0	41.0
28	36.34658765190198	38.0	35.0	40.0	30.0	41.0
29	36.336047025498765	38.0	35.0	40.0	30.0	41.0
30	35.90854733165436	38.0	35.0	40.0	29.0	41.0
31	35.92099410938895	38.0	35.0	40.0	29.0	41.0
32	35.85758174354278	38.0	35.0	40.0	29.0	41.0
33	35.775473476276936	38.0	35.0	40.0	28.0	41.0
34	35.633367587263216	38.0	35.0	40.0	27.0	41.0
35	35.67063515305117	38.0	35.0	40.0	28.0	41.0
36	35.493102181425144	38.0	34.0	40.0	27.0	41.0
37	35.461551294816296	38.0	34.0	40.0	27.0	41.0
38	35.30646795837704	38.0	34.0	40.0	26.0	41.0
39	35.1837182019704	38.0	34.0	40.0	26.0	41.0
40	35.06081936110209	38.0	34.0	40.0	25.0	41.0
41	34.81610254171259	38.0	34.0	40.0	24.0	41.0
42	34.800035141337396	38.0	34.0	40.0	24.0	41.0
43	34.70173346182994	38.0	33.0	40.0	24.0	41.0
44	34.62844602521302	37.0	33.0	40.0	24.0	41.0
45	34.59626862890215	37.0	33.0	40.0	24.0	41.0
46	33.20592468749945	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	12.0
11	15.0
12	12.0
13	7.0
14	15.0
15	17.0
16	44.0
17	97.0
18	222.0
19	513.0
20	844.0
21	1362.0
22	2090.0
23	2899.0
24	3975.0
25	5000.0
26	6343.0
27	7707.0
28	9826.0
29	11976.0
30	15314.0
31	19478.0
32	25403.0
33	32775.0
34	44077.0
35	54406.0
36	63130.0
37	80854.0
38	96196.0
39	78819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.13001762391315	21.850990080558855	19.956197565308756	21.06279473021924
2	24.151682790861123	33.13650634762592	21.489282779502304	21.22252808201065
3	25.662050372799893	33.137748718139854	22.174538858687455	19.025662050372798
4	23.017930956146095	26.539873881644688	27.990430197412675	22.451764964796546
5	23.65881665983363	33.58109751011201	19.97004112246401	22.79004470759035
6	18.387438569215124	42.95389563022794	21.87228786079771	16.786377939759227
7	75.1877310587304	6.8765207946201805	13.481849854198947	4.453898292450469
8	72.85083212202208	11.150097881048348	9.956712261664528	6.042357735265043
9	67.14817398156677	8.878689618574503	12.135652661601345	11.83748373825738
10	33.90251651021672	26.84904665811206	19.686425682283264	19.562011149387956
11	27.74674809517978	28.03107346136849	24.371049927321327	19.851128516130405
12	23.15157452714491	23.16346578777827	30.36797239807681	23.316987287000014
13	20.92453664016868	30.008394875044146	31.205507605969768	17.861560878817407
14	21.111957106270598	30.021173543187462	26.604832111373195	22.262037239168748
15	18.36986790051807	26.011866413223082	34.79489350222473	20.82337218403412
16	20.00571490436409	26.47225342938632	27.9416227843653	25.58040888188429
17	19.49829529017338	26.617255816512525	29.055496690857396	24.8289522024567
18	20.83224625913364	26.1211950184492	28.24209896723514	24.804459755182016
19	22.00202683875273	28.412126246141995	25.442860717841683	24.142986197263593
20	24.317982958226178	26.554072401803925	28.108455396236327	21.01948924373357
21	23.944916841042243	29.971656204132124	26.27169933213711	19.811727622688522
22	21.537735229545703	25.27727047648459	26.211000658456374	26.973993635513338
23	21.952332018195403	28.73780480229448	26.613528704970722	22.69633447453939
24	23.336865215222943	25.710680304345278	25.59354251303158	25.3589119674002
25	21.2922783122929	31.61726469058762	23.997983810137388	23.092473186982087
26	20.376828725026133	27.39551220274067	27.08740431528524	25.140254756947954
27	24.504161053814165	27.7110743132797	23.908000688628228	23.876763944277908
28	21.22927237908629	28.50051203413324	26.914359850844544	23.355855735935922
29	24.078915375045035	28.876950299854997	23.188135716554942	23.855998608545022
30	23.95059624910594	29.313022350245543	24.48233082906934	22.254050571579175
31	24.13499952967402	29.797014406173517	21.65008102030566	24.417905043846805
32	26.804853764116437	28.88564689345253	22.893161460246805	21.41633788218423
33	23.3823004797325	26.789057910439283	21.762426811065616	28.0662147987626
34	22.638120541886522	30.351821581395676	23.60078020868275	23.40927766803505
35	24.993477554801853	25.312944258384668	24.647743588924445	25.045834597889034
36	22.28209264889367	30.813628449574843	22.288481982965326	24.61579691856616
37	25.67216681841335	27.48141324970405	24.72778774632214	22.11863218556046
38	22.51707815752903	27.63706452694968	24.295797770477375	25.55005954504392
39	25.509238799586115	25.98400181741058	23.48027026883123	25.026489114172072
40	22.683200843392097	24.188776424777128	29.481629777136476	23.6463929546943
41	20.849107001822734	28.336519126294064	24.719623597230576	26.094750274652622
42	22.43082214756167	25.763037347432466	29.29491923704252	22.511221267963347
43	23.31237276794826	24.163396569992493	25.981872039386694	26.542358622672552
44	23.839670310361903	24.530605797610743	26.385110011909006	25.244613880118344
45	22.959007097485266	22.66385535967514	27.201169958061122	27.175967584778476
46	25.88248239827204	25.48545627831939	25.07139193417566	23.560669389232906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5352.0
1	4375.5
2	3399.0
3	4921.5
4	6444.0
5	4502.0
6	2560.0
7	2560.0
8	2448.0
9	2336.0
10	2332.5
11	2329.0
12	2332.0
13	2335.0
14	2321.0
15	2307.0
16	2167.5
17	2028.0
18	2093.0
19	2158.0
20	2158.0
21	2013.0
22	1868.0
23	2252.5
24	2637.0
25	3094.5
26	3552.0
27	4005.0
28	4458.0
29	5519.0
30	6580.0
31	6580.0
32	7219.5
33	7859.0
34	9161.5
35	10464.0
36	11640.0
37	12816.0
38	14690.5
39	16565.0
40	18995.0
41	21425.0
42	29164.0
43	36903.0
44	36903.0
45	38855.5
46	40808.0
47	53905.0
48	67002.0
49	59433.5
50	51865.0
51	47652.5
52	43440.0
53	39132.5
54	34825.0
55	32862.5
56	30900.0
57	30900.0
58	29901.5
59	28903.0
60	27766.0
61	26629.0
62	23478.0
63	20327.0
64	18813.5
65	17300.0
66	15252.5
67	13205.0
68	12192.0
69	11179.0
70	11179.0
71	9250.0
72	7321.0
73	6322.0
74	5323.0
75	4261.5
76	3200.0
77	2737.5
78	2275.0
79	1767.0
80	1259.0
81	1259.0
82	992.0
83	725.0
84	504.0
85	283.0
86	211.0
87	139.0
88	118.5
89	98.0
90	68.5
91	39.0
92	27.0
93	15.0
94	15.0
95	9.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	563439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.89593445108576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5281064399062	27.829438268836725
2	8.928411159079849	6.409873234373458
3	3.4217129018194092	3.6847674610243195
4	1.77377511611721	2.546852611964416
5	1.131619083336578	2.031026221952378
6	0.8008328508610196	1.7247986112469975
7	0.5998983997917335	1.5073739545364713
8	0.4912810465118119	1.4107993794119051
9	0.38475418753734353	1.2429999986059117
>10	4.720173895172991	37.46856311280797
>50	0.17347220016428647	3.8644324939152264
>100	0.0375609322292751	2.621526831131988
>500	0.002965336754942771	0.7921172972189434
>1k	0.003953782339923694	3.181597224708741
>5k	0.0014826683774713854	3.683833298264582
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	7956	1.4120428298360603	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA	6599	1.1712004316350129	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCG	6210	1.102160127360726	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4844	0.8597203956417643	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3046	0.5406086550629261	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	2719	0.48257220391204725	Illumina PCR Primer Index 7 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGT	1867	0.3313579642161796	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	1668	0.296039145320079	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATG	1569	0.278468476623024	No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	1125	0.1996666897392619	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	1096	0.19451972618153873	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATC	947	0.16807498238496094	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	906	0.16079824080335228	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	746	0.13240120048487947	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	708	0.12565690340924218	No Hit
GAATGATACCTGACTCTTATACACATCTGACGCAGAGCATCTCGTA	627	0.11128090174801532	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7748150199045504E-4	0.0	0.0	0.23427558262740067	0.0
2	1.7748150199045504E-4	0.0	0.0	1.1527423554280054	0.0
3	1.7748150199045504E-4	0.0	0.0	1.4283711280191822	0.0
4	1.7748150199045504E-4	0.0	0.0	2.074581276766429	0.0
5	1.7748150199045504E-4	0.0	0.0	4.303571460264554	0.0
6	1.7748150199045504E-4	0.0	0.0	5.344322987936582	0.0
7	1.7748150199045504E-4	0.0	0.0	6.339284288095073	0.0
8	1.7748150199045504E-4	0.0	0.0	7.8784038733563	0.0
9	1.7748150199045504E-4	0.0	0.0	8.409960971817712	0.0
10	1.7748150199045504E-4	0.0	0.0	10.660426417056682	0.0
11	1.7748150199045504E-4	0.0	0.0	11.655210235713183	0.0
12	1.7748150199045504E-4	0.0	0.0	13.895559235338697	0.0
13	1.7748150199045504E-4	0.0	0.0	14.477343598863408	0.0
14	1.7748150199045504E-4	0.0	0.0	14.767703336119792	0.0
15	1.7748150199045504E-4	0.0	0.0	15.280092432366237	0.0
16	1.7748150199045504E-4	0.0	0.0	15.947422879850347	0.0
17	1.7748150199045504E-4	0.0	0.0	16.639600737613122	0.0
18	1.7748150199045504E-4	0.0	0.0	17.593031366305848	0.0
19	1.7748150199045504E-4	0.0	0.0	18.069214236146237	0.0
20	1.7748150199045504E-4	0.0	0.0	18.509368361082565	0.0
21	1.7748150199045504E-4	0.0	0.0	18.925562483250182	0.0
22	1.7748150199045504E-4	0.0	0.0	19.34832342099145	0.0
23	1.7748150199045504E-4	0.0	0.0	19.772326729246643	0.0
24	0.0015973335179140953	0.0	0.0	20.11433358358225	0.0
25	0.0015973335179140953	0.0	0.0	20.407710506372474	0.0
26	0.0015973335179140953	0.0	0.0	20.670738092322328	0.0
27	0.0015973335179140953	0.0	0.0	21.002273538040498	0.0
28	0.0015973335179140953	0.0	0.0	21.31978794510142	0.0
29	0.0015973335179140953	0.0	0.0	21.629493166074766	0.0
30	0.0015973335179140953	0.0	0.0	22.042492621206556	0.0
31	0.0015973335179140953	0.0	0.0	22.3484707306381	0.0
32	0.0015973335179140953	0.0	0.0	22.6608381741413	0.0
33	0.0015973335179140953	0.0	0.0	22.959007097485266	0.0
34	0.0015973335179140953	0.0	0.0	23.235700759088385	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTACG	30	4.86309E-6	40.000004	1
TTACGCG	30	4.86309E-6	40.000004	1
TGCCGAT	30	4.86309E-6	40.000004	14
CTTAACG	35	3.0526826E-7	40.0	1
CGAGTAT	20	0.0012552239	40.0	29
TGCCACG	20	0.0012552239	40.0	1
TTGTCGT	20	0.0012552239	40.0	31
CACGAGT	20	0.0012552239	40.0	27
ATGTCGT	20	0.0012552239	40.0	39
TATGCGG	65	0.0	40.0	2
TCTAGCG	20	0.0012552239	40.0	1
CGTTATT	65	0.0	40.0	1
ATCCGTT	20	0.0012552239	40.0	34
CCGAGTG	20	0.0012552239	40.0	27
CGTACGG	35	3.0526826E-7	40.0	2
TCGTAAG	25	7.789588E-5	40.0	1
ATGCGGA	20	0.0012552239	40.0	12
CAGTACG	20	0.0012552239	40.0	1
ACGAGTA	20	0.0012552239	40.0	28
TTACCAG	20	0.0012552239	40.0	1
>>END_MODULE