FastQCFastQC Report
Sat 18 Jun 2016
SRR3553776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314117
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97980.7455957117973514No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51330.39060448955458305No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT21490.1635318620792517No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTC20250.1540958681761213No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC14510.11041634801163064No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC14340.10912270368620144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG304.8680467E-640.0000041
ATATACG451.2223609E-940.01
TATTACG200.001255834240.01
TCATACG200.001255834240.01
CGTTTTT38050.037.2667541
CATACGA1900.036.84210618
GACACGA3200.035.025
CACGACC3200.035.027
ACACGAC3150.034.28571326
ACGCATT701.8189894E-1234.28571317
ATCGACG351.4012199E-534.2857131
ACGACCA3250.033.84615328
CTAAGCG950.033.684211
CGACGTC302.2784375E-433.33333618
ACGTTAG302.2784375E-433.3333361
ACGCAAT302.2784375E-433.33333629
CAAATCG302.2784375E-433.3333361
CGTTTAG302.2784375E-433.3333361
CGACCAA3300.033.33333229
TGCGTAG900.033.3333321
Produced by FastQC (version 0.11.5)