##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553776_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1314117
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.751595938565593	31.0	31.0	34.0	30.0	34.0
2	31.914094407119002	33.0	31.0	34.0	30.0	34.0
3	31.826603719455726	33.0	31.0	34.0	30.0	34.0
4	35.6167289518361	37.0	35.0	37.0	33.0	37.0
5	35.42794210865547	37.0	35.0	37.0	33.0	37.0
6	35.42142822899331	37.0	35.0	37.0	33.0	37.0
7	35.77207280630264	37.0	35.0	37.0	35.0	37.0
8	35.56108550456314	37.0	35.0	37.0	35.0	37.0
9	37.298886628816156	39.0	37.0	39.0	34.0	39.0
10	36.80517412072137	39.0	37.0	39.0	32.0	39.0
11	36.7639799195962	39.0	37.0	39.0	32.0	39.0
12	36.775271151655446	39.0	35.0	39.0	33.0	39.0
13	35.142684403291334	39.0	35.0	39.0	30.0	39.0
14	36.910514056206566	39.0	36.0	41.0	30.0	41.0
15	37.53039950019671	40.0	36.0	41.0	32.0	41.0
16	37.716678195320505	40.0	36.0	41.0	33.0	41.0
17	37.74752324184224	39.0	37.0	41.0	33.0	41.0
18	37.764185380753766	39.0	37.0	41.0	33.0	41.0
19	37.70542196775477	39.0	36.0	41.0	33.0	41.0
20	37.66103626998205	39.0	36.0	41.0	33.0	41.0
21	37.534689833553635	39.0	36.0	41.0	32.0	41.0
22	37.54570179063204	39.0	36.0	41.0	33.0	41.0
23	37.47911259043145	39.0	36.0	41.0	32.0	41.0
24	37.424041390530675	39.0	35.0	41.0	32.0	41.0
25	37.211793927024765	39.0	35.0	41.0	32.0	41.0
26	37.19230936058205	39.0	35.0	41.0	32.0	41.0
27	37.16424945419624	39.0	35.0	41.0	32.0	41.0
28	37.01250116998715	39.0	35.0	41.0	31.0	41.0
29	36.9493743707752	39.0	35.0	41.0	31.0	41.0
30	36.80035491512552	39.0	35.0	40.0	31.0	41.0
31	36.71345702095019	39.0	35.0	40.0	31.0	41.0
32	36.58013631967321	39.0	35.0	40.0	30.0	41.0
33	36.428277695212834	39.0	35.0	40.0	30.0	41.0
34	36.315955124239316	39.0	35.0	40.0	30.0	41.0
35	36.203806053798864	39.0	35.0	40.0	30.0	41.0
36	36.087093462758645	39.0	35.0	40.0	29.0	41.0
37	36.03608126217072	39.0	35.0	40.0	29.0	41.0
38	35.9392740524626	38.0	35.0	40.0	29.0	41.0
39	35.83458170010737	38.0	35.0	40.0	29.0	41.0
40	35.734720728823994	38.0	35.0	40.0	28.0	41.0
41	35.60430768341023	38.0	35.0	40.0	28.0	41.0
42	35.51452572335644	38.0	34.0	40.0	27.0	41.0
43	35.41727715264318	38.0	34.0	40.0	27.0	41.0
44	35.312536859351184	38.0	34.0	40.0	27.0	41.0
45	35.237356338895246	38.0	34.0	40.0	26.0	41.0
46	34.220038246213996	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	29.0
10	51.0
11	41.0
12	29.0
13	21.0
14	32.0
15	44.0
16	97.0
17	203.0
18	330.0
19	665.0
20	1105.0
21	1877.0
22	2917.0
23	4228.0
24	6203.0
25	8332.0
26	11637.0
27	15677.0
28	19992.0
29	24075.0
30	28388.0
31	35296.0
32	44856.0
33	59604.0
34	86716.0
35	110761.0
36	131999.0
37	182796.0
38	269505.0
39	266603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.10683219226294	22.022468319030956	21.32953154095107	26.541167947755035
2	26.529829535726275	26.939382109812136	22.350901784239912	24.17988657022168
3	26.51704528592203	26.762000643778293	24.31267535539073	22.408278714908946
4	24.860495678847467	27.855206195490968	22.754975394123964	24.529322731537604
5	21.360731198211422	31.370113924407033	21.67683699396629	25.592317883415248
6	20.420784450699596	38.61254363195971	22.52029309414611	18.446378823194586
7	81.07093964997028	5.884255359302102	8.571611203568633	4.473193787158982
8	79.84677163448917	5.730768264926183	8.865877239241254	5.556582861343396
9	71.98453410160587	8.252765925712856	12.656407306198764	7.106292666482513
10	34.609094928381566	26.381212631751964	19.3510166902947	19.658675749571767
11	28.327234180822558	26.76063090272784	25.387693789822368	19.524441126627234
12	23.425463638321396	23.383838729732588	31.06960795728234	22.121089674663672
13	21.969961578763535	26.286091725470413	31.659966350028196	20.083980345737864
14	20.86800490367296	26.84730507253159	30.008515223530324	22.27617480026512
15	20.361124618279803	24.960867259155766	33.62896911005641	21.04903901250802
16	21.51467487293749	25.066641706940857	31.38411572181168	22.034567698309967
17	21.69540459487245	26.102546424709523	28.408581579874546	23.793467400543484
18	23.1626255500842	24.733946825130488	29.116281122609326	22.987146502175985
19	24.402012910570367	27.34756494284755	26.4997713293413	21.75065081724078
20	26.19728684736595	24.751068588261166	26.172859798632846	22.878784765740036
21	25.720769155257866	27.39375565493788	25.647411912333528	21.23806327747073
22	23.970772769852303	24.898467944635065	25.72434570133405	25.406413584178576
23	23.42105002826993	26.94120843121275	26.236781047654052	23.40096049286327
24	22.34199846741196	25.802268747759904	28.072690635613117	23.783042149215024
25	23.418614933069126	26.579292406992682	26.549158103882682	23.45293455605551
26	22.107848844509277	26.76253332085347	27.59594465332996	23.533673181307297
27	23.779313409688786	25.24265343192425	27.165769866762247	23.81226329162472
28	22.290404887844844	26.442698785572365	28.395645136620256	22.87125118996254
29	23.413820839392535	25.751360038718012	26.50585906734332	24.328960054546133
30	24.62961821512088	24.64255465837517	27.439718076853126	23.288109049650828
31	25.1972997838092	24.513798999632453	26.354122197642983	23.934779018915364
32	25.329936375528206	24.881726665129513	26.891593366496284	22.896743592845993
33	23.36603209607668	24.936592403872716	27.430434276400046	24.266941223650555
34	23.210414293400056	24.45429136066271	29.01765976697661	23.317634578960625
35	23.965978676175716	23.6434046587937	27.819136347828998	24.571480317201587
36	23.64774217212014	25.899215975442065	27.982896500083328	22.47014535235447
37	24.40246949092052	25.13802043501454	27.951544649372924	22.507965424692017
38	22.184858730234826	25.63112721317813	28.31444993101832	23.86956412556873
39	23.709532712840637	24.184528470448218	28.18318308034977	23.922755736361374
40	24.4744569927944	23.122674769445947	29.52142008664373	22.881448151115922
41	22.06378884071966	24.245558043918464	28.49731036125398	25.19334275410789
42	23.685257857557584	24.935755339897437	27.841128301361294	23.537858501183685
43	22.37943805612438	23.638077888041934	30.006460611954644	23.976023443879047
44	23.20280462089753	24.845961204367647	28.597529748112233	23.353704426622592
45	23.040262016243606	23.83318989100666	28.60491113043968	24.521636962310055
46	23.81233938834974	24.36274700045734	27.647690426347122	24.177223184845793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5598.0
1	4883.0
2	4168.0
3	10502.0
4	16836.0
5	11309.5
6	5783.0
7	5783.0
8	5365.0
9	4947.0
10	4934.0
11	4921.0
12	4898.0
13	4875.0
14	4628.5
15	4382.0
16	4143.5
17	3905.0
18	3739.5
19	3574.0
20	3574.0
21	4008.5
22	4443.0
23	5074.5
24	5706.0
25	6759.5
26	7813.0
27	9063.0
28	10313.0
29	13141.0
30	15969.0
31	15969.0
32	18924.5
33	21880.0
34	24666.5
35	27453.0
36	31221.5
37	34990.0
38	39543.0
39	44096.0
40	48431.5
41	52767.0
42	61602.5
43	70438.0
44	70438.0
45	83526.5
46	96615.0
47	108529.5
48	120444.0
49	122917.0
50	125390.0
51	117231.0
52	109072.0
53	97746.5
54	86421.0
55	82451.5
56	78482.0
57	78482.0
58	76244.0
59	74006.0
60	69652.0
61	65298.0
62	61001.5
63	56705.0
64	50506.5
65	44308.0
66	39489.5
67	34671.0
68	29884.0
69	25097.0
70	25097.0
71	20944.0
72	16791.0
73	13755.5
74	10720.0
75	8770.0
76	6820.0
77	5430.5
78	4041.0
79	3216.5
80	2392.0
81	2392.0
82	1790.0
83	1188.0
84	824.0
85	460.0
86	331.5
87	203.0
88	146.0
89	89.0
90	56.5
91	24.0
92	17.5
93	11.0
94	11.0
95	8.5
96	6.0
97	6.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1314117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.098018017377292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06823578585015	22.29307095197867
2	9.519011046549526	5.730067319733222
3	3.6388739626221653	3.2856868226990117
4	1.9739947281828714	2.3765331558022345
5	1.2940337846993992	1.9473926083488726
6	0.8864327215762593	1.6007920815117027
7	0.7309318576254821	1.539972015420077
8	0.5794840928858835	1.3953058134770289
9	0.5134087663179419	1.3907327669025178
>10	6.158955642424497	42.08626726722088
>50	0.5777031949866955	10.75719978223533
>100	0.05207566960605902	2.655973326815422
>500	0.004318470127524998	0.9184049583350031
>1k	0.0020322212364823522	0.8810173438972894
>5k	5.080553091205881E-4	1.141583785622705
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9798	0.7455957117973514	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5133	0.39060448955458305	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT	2149	0.1635318620792517	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTC	2025	0.1540958681761213	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	1451	0.11041634801163064	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC	1434	0.10912270368620144	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.609672502524509E-5	0.0	0.0	0.09032681260496592	0.0
2	7.609672502524509E-5	0.0	0.0	0.4484380005737693	0.0
3	7.609672502524509E-5	0.0	0.0	0.7716968884810105	0.0
4	7.609672502524509E-5	0.0	0.0	1.0678653422792643	0.0
5	7.609672502524509E-5	0.0	0.0	1.8147546984020448	0.0
6	7.609672502524509E-5	0.0	0.0	3.024844819753492	0.0
7	7.609672502524509E-5	0.0	0.0	3.9315372984292876	0.0
8	7.609672502524509E-5	0.0	0.0	5.774067301465547	0.0
9	7.609672502524509E-5	0.0	0.0	6.627568169348696	0.0
10	7.609672502524509E-5	0.0	0.0	7.907895567898445	0.0
11	7.609672502524509E-5	0.0	0.0	8.85956121106416	0.0
12	7.609672502524509E-5	0.0	0.0	9.71686691519857	0.0
13	7.609672502524509E-5	0.0	0.0	10.207538598161351	0.0
14	7.609672502524509E-5	0.0	0.0	10.421142105307213	0.0
15	7.609672502524509E-5	0.0	0.0	10.648366926232596	0.0
16	7.609672502524509E-5	0.0	0.0	11.1306679694426	0.0
17	7.609672502524509E-5	0.0	0.0	11.617458719429091	0.0
18	7.609672502524509E-5	0.0	0.0	12.322799263688088	0.0
19	7.609672502524509E-5	0.0	0.0	12.595453829453541	0.0
20	7.609672502524509E-5	0.0	0.0	12.926778970213459	0.0
21	7.609672502524509E-5	0.0	0.0	13.269518619727164	0.0
22	7.609672502524509E-5	0.0	0.0	13.60213740481251	0.0
23	7.609672502524509E-5	0.0	0.0	13.953628177704116	0.0
24	7.609672502524509E-5	0.0	0.0	14.234501189772296	0.0
25	7.609672502524509E-5	0.0	0.0	14.487370607031185	0.0
26	7.609672502524509E-5	0.0	0.0	14.732402061612474	0.0
27	7.609672502524509E-5	0.0	0.0	15.010687785029795	0.0
28	1.5219345005049019E-4	0.0	0.0	15.265916200764467	0.0
29	1.5219345005049019E-4	0.0	0.0	15.52129680994919	0.0
30	1.5219345005049019E-4	0.0	0.0	15.88313673744423	0.0
31	2.2829017507573527E-4	0.0	0.0	16.15632398028486	0.0
32	2.2829017507573527E-4	0.0	0.0	16.44298034345496	0.0
33	2.2829017507573527E-4	0.0	0.0	16.70992765484352	0.0
34	2.2829017507573527E-4	0.0	0.0	16.964927780403116	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	30	4.8680467E-6	40.000004	1
ATATACG	45	1.2223609E-9	40.0	1
TATTACG	20	0.0012558342	40.0	1
TCATACG	20	0.0012558342	40.0	1
CGTTTTT	3805	0.0	37.266754	1
CATACGA	190	0.0	36.842106	18
GACACGA	320	0.0	35.0	25
CACGACC	320	0.0	35.0	27
ACACGAC	315	0.0	34.285713	26
ACGCATT	70	1.8189894E-12	34.285713	17
ATCGACG	35	1.4012199E-5	34.285713	1
ACGACCA	325	0.0	33.846153	28
CTAAGCG	95	0.0	33.68421	1
CGACGTC	30	2.2784375E-4	33.333336	18
ACGTTAG	30	2.2784375E-4	33.333336	1
ACGCAAT	30	2.2784375E-4	33.333336	29
CAAATCG	30	2.2784375E-4	33.333336	1
CGTTTAG	30	2.2784375E-4	33.333336	1
CGACCAA	330	0.0	33.333332	29
TGCGTAG	90	0.0	33.333332	1
>>END_MODULE