FastQCFastQC Report
Sat 18 Jun 2016
SRR3553773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949481
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233772.4620819163311323No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29540.31111733673448966No Hit
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC25170.265092192471466No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC16810.17704409040307284No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC15320.16135130666121808No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT11770.1239624594910272No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG11350.11953899024835674No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.11553680379070248No Hit
CGTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGT10620.11185057942181044No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG10470.11027076897799956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG401.927765E-840.01
ATCGCCA507.8216544E-1140.024
CGTTTTT117500.038.9957431
CGTATGG750.037.3333322
CGTACGG650.036.9230772
CATCGCC552.1645974E-1036.36363623
GTTTTTT129650.035.2333222
CGGGTAT1600.035.06
GCCGATC408.697807E-735.09
AAGCACG408.697807E-735.01
TCGTAAG408.697807E-735.01
AATGCGG1500.034.6666682
GCGTACG351.4008116E-534.2857131
AAATGCG351.4008116E-534.2857131
CGCATGG2000.034.02
CTAAACG653.45608E-1133.8461531
AGTAGCG653.45608E-1133.8461531
CGCACTT3550.033.80281435
TCGTTTA605.511538E-1033.33333638
CATCGTT605.511538E-1033.33333636