##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553773_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	949481
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69283640220289	31.0	31.0	34.0	30.0	34.0
2	31.879212959501032	33.0	31.0	34.0	30.0	34.0
3	31.538359377386172	31.0	31.0	34.0	28.0	34.0
4	35.50294739968467	37.0	35.0	37.0	33.0	37.0
5	35.380120297299264	37.0	35.0	37.0	33.0	37.0
6	35.40037452039588	37.0	35.0	37.0	33.0	37.0
7	35.77946794090666	37.0	35.0	37.0	35.0	37.0
8	35.60589522065212	37.0	35.0	37.0	35.0	37.0
9	37.364904616311435	39.0	37.0	39.0	34.0	39.0
10	36.896700407907055	39.0	37.0	39.0	32.0	39.0
11	36.860640707923594	39.0	37.0	39.0	32.0	39.0
12	36.908427867434945	39.0	37.0	39.0	33.0	39.0
13	35.3725730162057	39.0	35.0	39.0	30.0	39.0
14	37.151608089050754	40.0	37.0	41.0	31.0	41.0
15	37.72889083615154	40.0	37.0	41.0	32.0	41.0
16	37.885679650251035	40.0	37.0	41.0	33.0	41.0
17	37.90363998858324	40.0	37.0	41.0	33.0	41.0
18	37.7868614537837	39.0	37.0	41.0	33.0	41.0
19	37.59515250963421	39.0	37.0	41.0	33.0	41.0
20	37.378940705501215	39.0	35.0	41.0	33.0	41.0
21	37.24081366557098	39.0	35.0	41.0	32.0	41.0
22	37.234580786766664	39.0	35.0	41.0	33.0	41.0
23	37.150232600757676	39.0	35.0	40.0	32.0	41.0
24	37.06300284049918	39.0	35.0	40.0	32.0	41.0
25	36.85201810252127	39.0	35.0	40.0	32.0	41.0
26	36.832969801396764	39.0	35.0	40.0	32.0	41.0
27	36.80225933957604	39.0	35.0	40.0	32.0	41.0
28	36.62375655752985	38.0	35.0	40.0	31.0	41.0
29	36.49359913468516	38.0	35.0	40.0	31.0	41.0
30	36.256391649753915	38.0	35.0	40.0	30.0	41.0
31	36.019373742075935	38.0	35.0	40.0	30.0	41.0
32	35.58933880720099	38.0	35.0	40.0	27.0	41.0
33	35.12567181439123	38.0	34.0	40.0	24.0	41.0
34	34.688643585285014	38.0	34.0	40.0	21.0	41.0
35	34.41060537283	38.0	34.0	40.0	20.0	41.0
36	34.17397925814208	38.0	33.0	40.0	18.0	41.0
37	34.07410048226347	38.0	33.0	40.0	18.0	41.0
38	33.89959883346797	38.0	33.0	40.0	18.0	41.0
39	33.77184904173964	38.0	33.0	40.0	16.0	41.0
40	33.63996435947639	38.0	33.0	40.0	16.0	41.0
41	33.54745592592163	37.0	33.0	40.0	15.0	41.0
42	33.42841826218745	37.0	33.0	40.0	15.0	41.0
43	33.330400503011646	37.0	32.0	40.0	15.0	41.0
44	33.19782597018792	37.0	32.0	40.0	15.0	41.0
45	33.144246172382594	37.0	32.0	40.0	15.0	41.0
46	32.312497037855415	36.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	19.0
10	22.0
11	21.0
12	20.0
13	21.0
14	12.0
15	32.0
16	72.0
17	162.0
18	337.0
19	655.0
20	1186.0
21	1974.0
22	3077.0
23	4570.0
24	6768.0
25	10030.0
26	15296.0
27	22814.0
28	28067.0
29	28419.0
30	27880.0
31	29421.0
32	34032.0
33	42508.0
34	59088.0
35	76485.0
36	90700.0
37	122108.0
38	175219.0
39	168462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.892606592443663	19.226398421874688	18.40573955666306	34.47525542901859
2	34.66209434417329	23.678093611141243	19.924674638039097	21.73513740664637
3	24.03639461979755	23.854505777366793	31.464031402418797	20.645068200416862
4	21.56757217890616	24.868533440900872	31.26286887257354	22.301025507619425
5	19.455576256923518	28.677035138143893	29.481263974739885	22.386124630192704
6	18.335490652261605	34.93297917493873	30.097811330611147	16.63371884218852
7	73.86551179012534	4.9699783355327805	17.228570134631447	3.935939739710431
8	73.00146079805704	4.950915289510796	17.341473921015798	4.706149991416363
9	65.91759076800905	7.296933798569956	20.573555447660354	6.211919985760642
10	29.854520522264266	25.734269564109237	27.04719736361233	17.364012550014166
11	24.355726970839857	24.761738254899253	33.31809693927524	17.56443783498564
12	20.506676805539026	21.754727056149623	37.38758332183583	20.351012816475528
13	19.83315095299432	23.93644527905245	37.96389817173804	18.266505596215197
14	19.163521966211015	24.11106699344168	36.664662062747965	20.060748977599342
15	18.448710400734715	23.09977766801021	39.55350344030054	18.89800849095453
16	19.800922819940578	23.337697120848127	37.54703885596447	19.314341203246826
17	19.29053872589341	23.74128602889368	35.52393360162026	21.444241643592658
18	20.67824421973689	23.01941797676836	36.014622725467916	20.28771507802684
19	21.92260824597859	24.987967110452974	33.86323686308626	19.226187780482178
20	23.087455146548482	23.000986854923898	33.66312754020354	20.248430458324073
21	23.030266008482528	24.920351223457867	33.7086260809853	18.340756687074308
22	22.02403207647125	23.087876429333498	33.131468665513054	21.756622828682197
23	20.5968313215325	24.9198246199766	33.551803564263004	20.9315404942279
24	20.036735858853415	23.488621678580195	35.40523717694193	21.069405285624462
25	20.82232293221244	24.77069051408085	33.59561697390469	20.81136957980202
26	19.87527923149594	24.622398973755136	34.56161839994692	20.940703394802
27	21.841616630559223	23.26586840600286	33.469337459096074	21.423177504341844
28	21.07140637885329	25.149845020595464	34.03248722196653	19.74626137858472
29	21.60338121563254	24.408597960359398	32.564527357577454	21.423493466430607
30	22.818676729708127	23.841024728246275	32.45520447486574	20.88509406717986
31	23.15338590240352	24.485482068624858	31.293938477968492	21.06719355100313
32	23.93075796145473	25.46033043315243	30.852223477878965	19.756688127513875
33	23.499996313775632	26.290362840330666	29.322440364788765	20.88720048110494
34	22.813831977680437	27.49375711572954	29.91529056400286	19.77712034258716
35	23.292409221458882	26.89511427822147	29.760258499116887	20.052218001202764
36	23.82954477235458	28.787095265729384	27.62172176167822	19.76163820023781
37	23.846817366540247	27.929152874043822	29.189420325419885	19.034609433996046
38	23.591625319516663	27.605607695151352	29.23955297683682	19.563214008495166
39	23.93918361715506	26.329647460033428	28.920220625794514	20.810948297017003
40	24.178366918348022	24.925722578966823	31.29383315727224	19.602077345412916
41	22.90862060430909	25.426522489654875	29.761838309560694	21.903018596475338
42	23.92559724733828	25.839168977578275	29.300323018575412	20.934910756508028
43	22.99814319612504	25.095078258543353	30.65485249309886	21.251926052232747
44	23.273767458221915	26.52259497557086	29.100845619870224	21.102791946337
45	22.129458093421565	25.116669001275433	30.505823707899367	22.248049197403635
46	22.922944218999643	25.644325689508268	29.22733577607135	22.20539431542074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3205.0
1	2842.0
2	2479.0
3	20928.5
4	39378.0
5	26879.5
6	14381.0
7	14381.0
8	13418.0
9	12455.0
10	12101.5
11	11748.0
12	11085.0
13	10422.0
14	9849.5
15	9277.0
16	8574.5
17	7872.0
18	7352.5
19	6833.0
20	6833.0
21	6483.5
22	6134.0
23	6146.0
24	6158.0
25	6737.0
26	7316.0
27	8011.0
28	8706.0
29	10266.5
30	11827.0
31	11827.0
32	13904.5
33	15982.0
34	17570.5
35	19159.0
36	21831.0
37	24503.0
38	27310.5
39	30118.0
40	34246.5
41	38375.0
42	43671.0
43	48967.0
44	48967.0
45	56616.0
46	64265.0
47	71588.0
48	78911.0
49	81381.5
50	83852.0
51	78392.5
52	72933.0
53	64939.5
54	56946.0
55	53433.5
56	49921.0
57	49921.0
58	47796.0
59	45671.0
60	43121.0
61	40571.0
62	37569.0
63	34567.0
64	30236.0
65	25905.0
66	22595.0
67	19285.0
68	16844.5
69	14404.0
70	14404.0
71	12248.5
72	10093.0
73	8327.0
74	6561.0
75	5534.0
76	4507.0
77	3621.5
78	2736.0
79	2189.5
80	1643.0
81	1643.0
82	1250.5
83	858.0
84	603.0
85	348.0
86	235.0
87	122.0
88	82.5
89	43.0
90	39.0
91	35.0
92	20.0
93	5.0
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	949481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.14443586610503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.48893356564606	32.5692745282368
2	9.160146248519505	7.904186846867847
3	3.811442493636511	4.933276086721436
4	2.225863538014623	3.8413450665029414
5	1.6106775052815092	3.474588616379806
6	1.198443765521812	3.1023708108413537
7	0.9607465583965586	2.9015607790625024
8	0.7780274864060228	2.6854045591449243
9	0.6624297911701532	2.5722143676844222
>10	4.017353708591474	27.65337888503307
>50	0.058426938690516406	1.7334566854390192
>100	0.022152782418535296	1.8804498375169423
>500	0.0029212460218397476	0.8015858540200933
>1k	0.0021909345163798107	1.4916302802402208
>5k	0.0	0.0
>10k+	2.4343716848664564E-4	2.4552767963086635
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23377	2.4620819163311323	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2954	0.31111733673448966	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	2517	0.265092192471466	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC	1681	0.17704409040307284	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC	1532	0.16135130666121808	No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	1177	0.1239624594910272	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	1135	0.11953899024835674	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1097	0.11553680379070248	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGT	1062	0.11185057942181044	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	1047	0.11027076897799956	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1064139250811758E-4	0.0	0.0	0.08162353959689557	0.0
2	2.1064139250811758E-4	0.0	0.0	0.3666213436603787	0.0
3	2.1064139250811758E-4	0.0	0.0	0.7888520149429004	0.0
4	2.1064139250811758E-4	0.0	0.0	1.0868042646456326	0.0
5	2.1064139250811758E-4	0.0	0.0	1.7233625528051641	0.0
6	2.1064139250811758E-4	0.0	0.0	2.8186977938473756	0.0
7	2.1064139250811758E-4	0.0	0.0	3.6731646025565547	0.0
8	2.1064139250811758E-4	0.0	0.0	5.402846397136962	0.0
9	2.1064139250811758E-4	0.0	0.0	6.271742141232947	0.0
10	2.1064139250811758E-4	0.0	0.0	7.535590496281653	0.0
11	2.1064139250811758E-4	0.0	0.0	8.419757741334477	0.0
12	2.1064139250811758E-4	0.0	0.0	9.244524113700011	0.0
13	2.1064139250811758E-4	0.0	0.0	9.788926792637241	0.0
14	2.1064139250811758E-4	0.0	0.0	10.052649816057404	0.0
15	2.1064139250811758E-4	0.0	0.0	10.2899373447178	0.0
16	2.1064139250811758E-4	0.0	0.0	10.685311238455535	0.0
17	2.1064139250811758E-4	0.0	0.0	11.092375729477473	0.0
18	2.1064139250811758E-4	0.0	0.0	11.674588538369909	0.0
19	2.1064139250811758E-4	0.0	0.0	11.919459157160595	0.0
20	2.1064139250811758E-4	0.0	0.0	12.195504702042484	0.0
21	2.1064139250811758E-4	0.0	0.0	12.473761981545708	0.0
22	2.1064139250811758E-4	0.0	0.0	12.7745578900473	0.0
23	2.1064139250811758E-4	0.0	0.0	13.101368010523643	0.0
24	2.1064139250811758E-4	0.0	0.0	13.367724051350159	0.0
25	2.1064139250811758E-4	0.0	0.0	13.606064786973093	0.0
26	2.1064139250811758E-4	0.0	0.0	13.83924480847958	0.0
27	2.1064139250811758E-4	0.0	0.0	14.122662802099253	0.0
28	2.1064139250811758E-4	0.0	0.0	14.373220738487658	0.0
29	3.159620887621764E-4	0.0	0.0	14.634521385893978	0.0
30	3.159620887621764E-4	0.0	0.0	14.987029756256312	0.0
31	3.159620887621764E-4	0.0	0.0	15.250752779676477	0.0
32	3.159620887621764E-4	0.0	0.0	15.521953572530677	0.0
33	3.159620887621764E-4	0.0	0.0	15.78904685823097	0.0
34	3.159620887621764E-4	0.0	0.0	16.030125931956512	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	40	1.927765E-8	40.0	1
ATCGCCA	50	7.8216544E-11	40.0	24
CGTTTTT	11750	0.0	38.995743	1
CGTATGG	75	0.0	37.333332	2
CGTACGG	65	0.0	36.923077	2
CATCGCC	55	2.1645974E-10	36.363636	23
GTTTTTT	12965	0.0	35.233322	2
CGGGTAT	160	0.0	35.0	6
GCCGATC	40	8.697807E-7	35.0	9
AAGCACG	40	8.697807E-7	35.0	1
TCGTAAG	40	8.697807E-7	35.0	1
AATGCGG	150	0.0	34.666668	2
GCGTACG	35	1.4008116E-5	34.285713	1
AAATGCG	35	1.4008116E-5	34.285713	1
CGCATGG	200	0.0	34.0	2
CTAAACG	65	3.45608E-11	33.846153	1
AGTAGCG	65	3.45608E-11	33.846153	1
CGCACTT	355	0.0	33.802814	35
TCGTTTA	60	5.511538E-10	33.333336	38
CATCGTT	60	5.511538E-10	33.333336	36
>>END_MODULE