FastQCFastQC Report
Sat 18 Jun 2016
SRR3553772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031426
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183941.7833562465945207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39900.3868430696918635No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG200.001255708640.0000041
TACGTCG257.794095E-540.0000041
CGTTTTT89350.038.679351
CGTAAGG1250.036.82
CTCGTAG408.698771E-735.0000041
ACGTAAG408.698771E-735.0000041
CTTACCG351.4009289E-534.2857131
GTTTTTT102450.034.202052
CGTTCAT653.45608E-1133.84615317
CGAATAT653.45608E-1133.84615314
TAGGGCG5200.033.8461535
CATACGA1950.033.84615318
AGGGCGC1950.033.8461536
GGGCGAA2550.033.7254877
AACCGGT302.2781E-433.33333230
GCCGATT900.033.3333329
ATACGAG302.2781E-433.3333321
TCAACGT302.2781E-433.33333219
ACGGTCG302.2781E-433.3333325
TCATGCG558.682036E-932.7272721
Produced by FastQC (version 0.11.5)