FastQCFastQC Report
Sat 18 Jun 2016
SRR3553768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853251
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138731.6258990613547477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16760.19642520196284566No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG16590.19443282222933228No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAC200.001255586740.00000429
CGTTAGT200.001255586740.00000428
CTTACCG200.001255586740.0000041
ACGCGAG257.7929624E-540.01
ATAACGC257.7929624E-540.011
GCGTACG257.7929624E-540.01
CGTTTTT65400.038.7767561
AAGGGCG5850.036.2393155
TATAGCG455.4396878E-835.5555571
TTACGAG455.4396878E-835.5555571
GCGTAAG455.4396878E-835.5555571
CGTTAGG800.035.0000042
GCGAAGT351.4006457E-534.2857139
TCCGTCA351.4006457E-534.28571329
TATCGCG351.4006457E-534.2857131
TACGTAG351.4006457E-534.2857131
AGGGCGA14250.034.2456136
TTAACGG950.033.684212
CACGACC3150.033.6507927
ACCGCTA900.033.33333626
Produced by FastQC (version 0.11.5)