##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553768_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	853251
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.876511132128766	31.0	31.0	34.0	30.0	34.0
2	32.05105180070108	33.0	31.0	34.0	30.0	34.0
3	31.846229304155518	33.0	31.0	34.0	30.0	34.0
4	35.703769465257	37.0	35.0	37.0	33.0	37.0
5	35.54590618704226	37.0	35.0	37.0	33.0	37.0
6	35.55373155144266	37.0	35.0	37.0	33.0	37.0
7	35.831438814604375	37.0	35.0	37.0	35.0	37.0
8	35.608108282322554	37.0	35.0	37.0	35.0	37.0
9	37.39623393350843	39.0	37.0	39.0	35.0	39.0
10	36.99114797404281	39.0	37.0	39.0	33.0	39.0
11	36.96748553473714	39.0	37.0	39.0	33.0	39.0
12	36.95449756285079	39.0	37.0	39.0	33.0	39.0
13	35.24919748116322	39.0	35.0	39.0	30.0	39.0
14	37.06134654398296	40.0	36.0	41.0	31.0	41.0
15	37.71328600845472	40.0	37.0	41.0	32.0	41.0
16	37.9121887932156	40.0	37.0	41.0	33.0	41.0
17	37.9456619447267	40.0	37.0	41.0	33.0	41.0
18	37.902551535245784	39.0	37.0	41.0	33.0	41.0
19	37.805207377430555	39.0	37.0	41.0	33.0	41.0
20	37.659514023423355	39.0	35.0	41.0	33.0	41.0
21	37.53414294269799	39.0	35.0	41.0	33.0	41.0
22	37.53359562426531	39.0	35.0	41.0	33.0	41.0
23	37.455461523045386	39.0	35.0	41.0	33.0	41.0
24	37.37641561510036	39.0	35.0	41.0	33.0	41.0
25	37.173040523831794	39.0	35.0	41.0	32.0	41.0
26	37.16380174180868	39.0	35.0	41.0	32.0	41.0
27	37.130629791233766	39.0	35.0	41.0	32.0	41.0
28	36.985419296314916	39.0	35.0	41.0	32.0	41.0
29	36.89033824747935	39.0	35.0	41.0	31.0	41.0
30	36.720680081242214	39.0	35.0	41.0	31.0	41.0
31	36.58472712015573	39.0	35.0	41.0	31.0	41.0
32	36.267561362365825	39.0	35.0	40.0	30.0	41.0
33	35.9801781656277	39.0	35.0	40.0	29.0	41.0
34	35.665888466582516	39.0	35.0	40.0	26.0	41.0
35	35.459578717165286	39.0	35.0	40.0	25.0	41.0
36	35.3084238987121	38.0	35.0	40.0	25.0	41.0
37	35.20006774091094	38.0	35.0	40.0	24.0	41.0
38	35.07174911016805	38.0	34.0	40.0	24.0	41.0
39	34.935811384926595	38.0	34.0	40.0	23.0	41.0
40	34.83803007555807	38.0	34.0	40.0	23.0	41.0
41	34.72541256910335	38.0	34.0	40.0	23.0	41.0
42	34.65389668456292	38.0	34.0	40.0	23.0	41.0
43	34.57839955651971	38.0	34.0	40.0	22.0	41.0
44	34.49377967327317	38.0	34.0	40.0	22.0	41.0
45	34.41138773936392	38.0	34.0	40.0	22.0	41.0
46	33.548149372224586	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	20.0
10	38.0
11	40.0
12	12.0
13	17.0
14	23.0
15	39.0
16	65.0
17	114.0
18	240.0
19	465.0
20	889.0
21	1458.0
22	2257.0
23	3299.0
24	4848.0
25	6881.0
26	10081.0
27	14425.0
28	18110.0
29	19702.0
30	20601.0
31	22972.0
32	27963.0
33	36698.0
34	53610.0
35	66951.0
36	80981.0
37	113021.0
38	168883.0
39	178541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.653814645397425	19.870237479944354	19.99306183057506	27.48288604408316
2	31.23992822745007	24.64011176078317	21.143954123698656	22.97600588806811
3	26.90005637262658	24.765045689955244	26.955843005164954	21.37905493225323
4	23.792295584769313	25.246967187849766	27.06214232388828	23.898594903492643
5	21.054883029729822	29.307905879981387	25.55906761316424	24.07814347712455
6	19.57196651395662	37.0355264746247	26.637999838265646	16.754507173153037
7	77.74828274446793	5.220152100612832	13.161308923165635	3.8702562317536104
8	76.68400037034824	5.708519532939311	13.047157284316105	4.560322812396352
9	69.38087385775113	7.479276320801265	16.059869839003998	7.079979982443618
10	32.76093435577573	26.085055862811764	22.76000848519369	18.394001296218814
11	27.883119972903636	26.105565654186165	28.307965651373397	17.703348721536805
12	22.758016105460175	24.331761697320015	33.14921400619512	19.761008191024683
13	21.525553442070386	26.58151001288015	33.748803107174794	18.14413343787467
14	20.758252847052038	25.845735897174453	33.388768369448144	20.00724288632536
15	19.730536500982712	24.67691218644924	35.70039765555505	19.892153657013
16	22.343835518505106	24.800908525158484	33.086278246377674	19.768977709958733
17	22.772548757634038	24.72472930005356	31.442916562652723	21.059805379659675
18	23.63560077866888	23.125317169273753	32.40078241924123	20.838299632816135
19	24.440404992200417	25.333577106853667	29.91276892731447	20.31324897363144
20	26.41918966400274	23.93785650412364	30.06676816083427	19.576185671039354
21	26.01333019240528	26.00125871519635	30.153378079838173	17.832033012560196
22	24.782508312325447	23.57875935685982	30.298587402768938	21.34014492804579
23	22.876269702584583	26.00032112473352	30.598264754450916	20.52514441823098
24	22.149871491507188	24.95150899325052	31.76087692835988	21.137742586882407
25	22.58737464122515	26.309960375083065	30.376407411183813	20.726257572507972
26	21.59106757566062	25.17629630671397	31.482529759707283	21.750106357918128
27	22.81532632250065	24.973073573895608	30.800198300382885	21.41140180322086
28	21.584152845997252	26.36844258020207	31.21121451952591	20.836190054274766
29	22.687814019555795	25.74459332599669	30.520796342459604	21.046796311987915
30	22.754968936455978	24.83313819731826	31.159529845262412	21.25236302096335
31	23.946880812328377	24.818371147528687	29.494720779700227	21.74002726044271
32	23.78374007179599	25.646029128591703	29.6069972376241	20.963233561988208
33	23.541138539538775	26.211396177678083	28.17892976392644	22.068535518856702
34	22.174366042348616	26.24936859142269	30.254403452208084	21.32186191402061
35	22.700119894380435	27.909724102286432	28.440751900671668	20.949404102661468
36	23.13270069416854	28.84801775796337	26.45716207774734	21.56211947012075
37	23.225170553565132	29.352793023389367	26.300350072839056	21.121686350206446
38	22.556316957143913	28.936971653124345	27.203132489736316	21.30357889999543
39	23.296779025163755	27.697945856494748	26.881187364562127	22.124087753779367
40	23.62306050622853	26.515644282866358	28.872160712381234	20.98913449852388
41	21.806010189264356	27.47690890488262	28.326483063014287	22.39059784283874
42	23.033081707492872	26.92595730916225	28.349336830545756	21.69162415279912
43	22.903694223622356	25.771080256571633	28.902046408383935	22.42317911142208
44	23.453122234840627	26.08904062227879	28.318513544080233	22.139323598800352
45	22.777939902795307	25.75818838770772	28.665773611750822	22.79809809774615
46	22.37512760020205	26.878843388405055	28.300933722902172	22.445095288490727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1911.0
1	1960.5
2	2010.0
3	13083.0
4	24156.0
5	16438.5
6	8721.0
7	8721.0
8	7992.0
9	7263.0
10	6999.0
11	6735.0
12	6425.0
13	6115.0
14	5792.5
15	5470.0
16	5108.5
17	4747.0
18	4515.0
19	4283.0
20	4283.0
21	4281.5
22	4280.0
23	4843.5
24	5407.0
25	6207.5
26	7008.0
27	8217.5
28	9427.0
29	11376.5
30	13326.0
31	13326.0
32	14360.5
33	15395.0
34	18028.0
35	20661.0
36	23264.0
37	25867.0
38	28811.5
39	31756.0
40	34570.0
41	37384.0
42	42006.5
43	46629.0
44	46629.0
45	50893.0
46	55157.0
47	60570.5
48	65984.0
49	67590.0
50	69196.0
51	65724.0
52	62252.0
53	59424.5
54	56597.0
55	53786.0
56	50975.0
57	50975.0
58	48812.0
59	46649.0
60	43161.0
61	39673.0
62	36560.5
63	33448.0
64	29410.0
65	25372.0
66	22833.0
67	20294.0
68	17303.0
69	14312.0
70	14312.0
71	12015.0
72	9718.0
73	7842.0
74	5966.0
75	4922.5
76	3879.0
77	3238.5
78	2598.0
79	2034.0
80	1470.0
81	1470.0
82	1085.0
83	700.0
84	484.5
85	269.0
86	183.0
87	97.0
88	65.5
89	34.0
90	34.0
91	34.0
92	25.0
93	16.0
94	16.0
95	12.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	853251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.547109673447494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92972300330352	25.924408620525764
2	8.846220183508278	6.289151181172671
3	3.7145366343886725	3.9612312338595794
4	2.2110947700962726	3.143921131639935
5	1.5829236396787454	2.813418011217652
6	1.2767863710761078	2.7231639097323255
7	1.0237011582103828	2.5472732140617786
8	0.8991280148569916	2.556912172367248
9	0.8034381387761007	2.570391327141412
>10	6.612206943240831	41.26641172036422
>50	0.06748263465333842	1.5522409280555352
>100	0.030137827553941517	2.237529650911151
>500	0.001637925410536706	0.41012643406195565
>1k	6.551701642146824E-4	0.38835083974918283
>5k	0.0	0.0
>10k+	3.275850821073412E-4	1.6154696251395544
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13873	1.6258990613547477	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1676	0.19642520196284566	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	1659	0.19443282222933228	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1719880785372651E-4	0.0	0.0	0.055083439691251464	0.0
2	1.1719880785372651E-4	0.0	0.0	0.22877207293047416	0.0
3	1.1719880785372651E-4	0.0	0.0	0.3695278411627997	0.0
4	1.1719880785372651E-4	0.0	0.0	0.5448572577119746	0.0
5	1.1719880785372651E-4	0.0	0.0	0.9984166441058961	0.0
6	1.1719880785372651E-4	0.0	0.0	1.6002325224347818	0.0
7	1.1719880785372651E-4	0.0	0.0	2.0409000399647934	0.0
8	1.1719880785372651E-4	0.0	0.0	3.010485777338673	0.0
9	1.1719880785372651E-4	0.0	0.0	3.505885138136375	0.0
10	1.1719880785372651E-4	0.0	0.0	4.325690799073191	0.0
11	1.1719880785372651E-4	0.0	0.0	4.835622812044756	0.0
12	1.1719880785372651E-4	0.0	0.0	5.35657151295457	0.0
13	1.1719880785372651E-4	0.0	0.0	5.624136391284628	0.0
14	1.1719880785372651E-4	0.0	0.0	5.763602972630562	0.0
15	1.1719880785372651E-4	0.0	0.0	5.904007144439327	0.0
16	1.1719880785372651E-4	0.0	0.0	6.133599609024777	0.0
17	1.1719880785372651E-4	0.0	0.0	6.360379302221737	0.0
18	1.1719880785372651E-4	0.0	0.0	6.686074789247244	0.0
19	1.1719880785372651E-4	0.0	0.0	6.846285559583288	0.0
20	1.1719880785372651E-4	0.0	0.0	7.0115358786570425	0.0
21	1.1719880785372651E-4	0.0	0.0	7.174676619189429	0.0
22	1.1719880785372651E-4	0.0	0.0	7.349654439315043	0.0
23	1.1719880785372651E-4	0.0	0.0	7.529085814139099	0.0
24	1.1719880785372651E-4	0.0	0.0	7.672888751375621	0.0
25	1.1719880785372651E-4	0.0	0.0	7.824661207546197	0.0
26	1.1719880785372651E-4	0.0	0.0	7.966354566241352	0.0
27	1.1719880785372651E-4	0.0	0.0	8.148247116030335	0.0
28	1.1719880785372651E-4	0.0	0.0	8.300605566240181	0.0
29	1.1719880785372651E-4	0.0	0.0	8.459527149689833	0.0
30	1.1719880785372651E-4	0.0	0.0	8.701777085523487	0.0
31	1.1719880785372651E-4	0.0	0.0	8.866558609365825	0.0
32	1.1719880785372651E-4	0.0	0.0	9.055248690010325	0.0
33	1.1719880785372651E-4	0.0	0.0	9.22870292563384	0.0
34	1.1719880785372651E-4	0.0	0.0	9.40227436006521	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAC	20	0.0012555867	40.000004	29
CGTTAGT	20	0.0012555867	40.000004	28
CTTACCG	20	0.0012555867	40.000004	1
ACGCGAG	25	7.7929624E-5	40.0	1
ATAACGC	25	7.7929624E-5	40.0	11
GCGTACG	25	7.7929624E-5	40.0	1
CGTTTTT	6540	0.0	38.776756	1
AAGGGCG	585	0.0	36.239315	5
TATAGCG	45	5.4396878E-8	35.555557	1
TTACGAG	45	5.4396878E-8	35.555557	1
GCGTAAG	45	5.4396878E-8	35.555557	1
CGTTAGG	80	0.0	35.000004	2
GCGAAGT	35	1.4006457E-5	34.285713	9
TCCGTCA	35	1.4006457E-5	34.285713	29
TATCGCG	35	1.4006457E-5	34.285713	1
TACGTAG	35	1.4006457E-5	34.285713	1
AGGGCGA	1425	0.0	34.245613	6
TTAACGG	95	0.0	33.68421	2
CACGACC	315	0.0	33.65079	27
ACCGCTA	90	0.0	33.333336	26
>>END_MODULE