FastQCFastQC Report
Sat 18 Jun 2016
SRR3553767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739078
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120981.6369043592151304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22250.301050768660412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG304.865151E-640.0000041
TACGGGT1100.040.04
CGTTTTT59800.038.461541
CACGACC2700.037.0370427
GCGAGAC2750.036.36363621
CGATCGG552.1645974E-1036.36363610
TCGACGG552.1645974E-1036.3636362
ACGGGTA950.035.7894745
CGATTGT455.4380507E-835.55555710
ACACGAC3000.035.33333226
GCGATCG1200.035.0000049
ACCGGGT800.035.04
CTCGTAG408.694333E-735.01
AGACACG2950.034.57627524
CTTAACG351.4003928E-534.2857131
ACGCATG351.4003928E-534.2857131
CTATACG351.4003928E-534.2857131
AAACGCG351.4003928E-534.2857131
CGAATAT351.4003928E-534.28571314
TCGTACG351.4003928E-534.28571330
Produced by FastQC (version 0.11.5)