##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553767_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	739078
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.859083885598	31.0	31.0	34.0	30.0	34.0
2	32.02948132673412	33.0	31.0	34.0	30.0	34.0
3	31.817791897472254	33.0	31.0	34.0	30.0	34.0
4	35.67471633575888	37.0	35.0	37.0	33.0	37.0
5	35.5361206800906	37.0	35.0	37.0	33.0	37.0
6	35.54001607408149	37.0	35.0	37.0	33.0	37.0
7	35.8373486966193	37.0	35.0	37.0	35.0	37.0
8	35.65896021800135	37.0	35.0	37.0	35.0	37.0
9	37.433188377951986	39.0	37.0	39.0	35.0	39.0
10	36.98148098035661	39.0	37.0	39.0	33.0	39.0
11	36.944208865640704	39.0	37.0	39.0	33.0	39.0
12	36.9689261485256	39.0	37.0	39.0	33.0	39.0
13	35.25363087522562	39.0	35.0	39.0	30.0	39.0
14	37.1379705524992	40.0	37.0	41.0	31.0	41.0
15	37.796599276395725	40.0	37.0	41.0	32.0	41.0
16	37.977113376396	40.0	37.0	41.0	33.0	41.0
17	38.01084188678326	40.0	37.0	41.0	33.0	41.0
18	37.96212307767245	39.0	37.0	41.0	33.0	41.0
19	37.84532890980384	39.0	37.0	41.0	33.0	41.0
20	37.7009233125597	39.0	36.0	41.0	33.0	41.0
21	37.560909673945105	39.0	35.0	41.0	33.0	41.0
22	37.54958881200631	39.0	35.0	41.0	33.0	41.0
23	37.47512441176709	39.0	35.0	41.0	33.0	41.0
24	37.39509226360411	39.0	35.0	41.0	33.0	41.0
25	37.19308516827723	39.0	35.0	41.0	32.0	41.0
26	37.189051764495765	39.0	35.0	41.0	32.0	41.0
27	37.152952732999765	39.0	35.0	41.0	32.0	41.0
28	36.99564322033669	39.0	35.0	41.0	32.0	41.0
29	36.91026928145608	39.0	35.0	41.0	32.0	41.0
30	36.71901747853406	39.0	35.0	40.0	31.0	41.0
31	36.572674332073206	39.0	35.0	40.0	31.0	41.0
32	36.302275267292494	39.0	35.0	40.0	30.0	41.0
33	35.99441736866745	39.0	35.0	40.0	29.0	41.0
34	35.698850459626726	39.0	35.0	40.0	27.0	41.0
35	35.50712509369782	39.0	35.0	40.0	26.0	41.0
36	35.30562674034405	38.0	35.0	40.0	25.0	41.0
37	35.19490094414933	38.0	35.0	40.0	24.0	41.0
38	35.055366280690265	38.0	34.0	40.0	24.0	41.0
39	34.941726042447485	38.0	34.0	40.0	23.0	41.0
40	34.79530036071971	38.0	34.0	40.0	23.0	41.0
41	34.706615539902415	38.0	34.0	40.0	23.0	41.0
42	34.607067183707265	38.0	34.0	40.0	23.0	41.0
43	34.54775138753961	38.0	34.0	40.0	22.0	41.0
44	34.425504479906046	38.0	34.0	40.0	22.0	41.0
45	34.34376885795545	37.0	33.0	40.0	22.0	41.0
46	33.474374829179055	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	14.0
10	29.0
11	26.0
12	14.0
13	13.0
14	17.0
15	36.0
16	43.0
17	97.0
18	205.0
19	347.0
20	659.0
21	1196.0
22	1758.0
23	2807.0
24	4030.0
25	5967.0
26	8798.0
27	12839.0
28	15588.0
29	16518.0
30	17255.0
31	19533.0
32	23841.0
33	31247.0
34	45946.0
35	60146.0
36	73118.0
37	101082.0
38	147415.0
39	148491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.405045204971596	21.10967989846809	20.66899569463575	27.81627920192456
2	30.521000489799455	25.055136264372635	21.946398079769658	22.47746516605825
3	26.14974332885027	24.913337969740677	27.958483407705277	20.978435293703775
4	23.42039676461754	26.372182638368347	26.953718010818882	23.25370258619523
5	20.663312938553172	30.08884042009098	25.5660430969397	23.68180354441615
6	19.4827880142556	37.051840265844746	25.99454996630938	17.470821753590286
7	78.21204257196128	5.679914704537275	12.413439447527866	3.6946032759735776
8	77.83089200327976	5.294434416935696	12.508693263769183	4.365980316015359
9	70.17229575227513	7.851133439231043	15.766400839965469	6.210169968528356
10	32.50834147410693	26.186140028522026	22.790693269181332	18.514825228189718
11	26.95926546318521	26.469601314069692	28.270899688530843	18.30023353421425
12	22.006743537217993	24.26672150977299	33.47008028922522	20.256454663783796
13	21.258919897493904	26.52602296374672	33.63866871967505	18.57638841908432
14	20.89982383456144	26.019581153815967	32.69289574307448	20.38769926854811
15	20.25117781884997	24.40933162670246	35.27354352314641	20.065947031301164
16	21.74114234221557	24.549370973023148	33.7718075764669	19.937679108294386
17	21.65360083780061	24.631365025071776	31.359342315696043	22.355691821431567
18	22.895553649276533	24.39214805473847	31.2984556433827	21.4138426526023
19	24.157396107041475	26.347692665726758	29.748957484866278	19.745953742365487
20	26.190605051158332	24.05835378674511	29.26944111446965	20.48160004762691
21	25.760745144626142	25.963565415287697	29.22303193979526	19.052657500290906
22	24.398372025686058	24.369281726691906	29.37010707936104	21.862239168260995
23	23.114069151023305	26.057601498082743	29.485927060472644	21.342402290421308
24	21.318182925212223	25.21222387894106	31.706666955314596	21.76292624053212
25	22.637123551235458	26.109828732555968	29.903880239974672	21.3491674762339
26	21.620451427318905	26.287753119427176	30.741139636141245	21.350655817112674
27	22.527798148503948	25.24997361577533	30.387320418142604	21.834907817578117
28	21.817318334465373	26.33253864950655	31.164369660577094	20.68577335545098
29	22.357856680891597	26.034329258887425	29.51961768581936	22.08819637440162
30	22.62291666102901	25.397184059057366	30.51274696310809	21.46715231680553
31	22.793940558371375	26.755362762793645	28.26968195508458	22.1810147237504
32	22.862404238794824	26.27557578496451	29.536801257783345	21.32521871845732
33	22.996219614167924	27.645931823163455	27.08793929734074	22.269909265327883
34	22.11349816934072	27.556225459288463	28.1917470145235	22.138529356847314
35	23.498872920043624	27.203218063587336	27.503592313666488	21.794316702702556
36	23.588038069053603	29.526518175348205	25.765616078411206	21.11982767718698
37	24.216388527327293	29.62312502875204	25.923786122709647	20.236700321211025
38	23.824413661345623	28.60834715686301	26.868611973296456	20.69862720849491
39	24.519874762880235	27.042477248680115	26.5929983032914	21.84464968514825
40	24.276733984775625	26.62628301748936	28.74365087311488	20.353332124620135
41	22.310770987635948	27.121224011538704	28.017746435423597	22.550258565401755
42	23.130440900689777	26.933422453381105	27.973637423925485	21.962499222003633
43	23.286987300393193	25.523016515171605	28.86434178801155	22.32565439642365
44	23.55001772478683	27.05681944260281	27.43025769945797	21.96290513315239
45	22.863892579673593	26.232549203196413	28.108399925312348	22.795158291817643
46	22.604515355618755	26.92814560844728	27.855923190786356	22.611415845147604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2516.0
1	2329.0
2	2142.0
3	11118.5
4	20095.0
5	13658.5
6	7222.0
7	7222.0
8	6736.5
9	6251.0
10	6062.5
11	5874.0
12	5687.5
13	5501.0
14	5297.5
15	5094.0
16	4733.0
17	4372.0
18	4266.0
19	4160.0
20	4160.0
21	4073.5
22	3987.0
23	4183.0
24	4379.0
25	5367.0
26	6355.0
27	6985.0
28	7615.0
29	9058.0
30	10501.0
31	10501.0
32	11825.0
33	13149.0
34	14838.5
35	16528.0
36	18629.0
37	20730.0
38	23715.0
39	26700.0
40	29718.0
41	32736.0
42	37008.5
43	41281.0
44	41281.0
45	45592.5
46	49904.0
47	53898.0
48	57892.0
49	58661.0
50	59430.0
51	57121.5
52	54813.0
53	50368.0
54	45923.0
55	44469.5
56	43016.0
57	43016.0
58	41690.5
59	40365.0
60	37839.0
61	35313.0
62	32373.0
63	29433.0
64	26592.0
65	23751.0
66	20286.5
67	16822.0
68	14551.5
69	12281.0
70	12281.0
71	10530.5
72	8780.0
73	7216.5
74	5653.0
75	4788.5
76	3924.0
77	3132.5
78	2341.0
79	1729.5
80	1118.0
81	1118.0
82	919.0
83	720.0
84	523.0
85	326.0
86	192.5
87	59.0
88	36.5
89	14.0
90	10.0
91	6.0
92	4.0
93	2.0
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	739078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.24932661103614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.06066466732084	27.208985491671733
2	8.907262770284166	6.457645547407067
3	3.6144559891223498	3.9306478701273533
4	2.026296347616338	2.9380751246197696
5	1.3213763279519242	2.3949501044010457
6	1.0370695081665742	2.2555842791926057
7	0.8362651496093887	2.121983397913241
8	0.6834744388725003	1.9820390531987135
9	0.5826861946533315	1.9009783963557352
>10	5.777808760457204	41.49731290864669
>50	0.12054442696172792	2.777097925388594
>100	0.029483001159375334	2.215942254762779
>500	0.001866012731606031	0.3810933657916205
>1k	3.7320254632120626E-4	0.3010055871091074
>5k	0.0	0.0
>10k+	3.7320254632120626E-4	1.6366586934139244
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12098	1.6369043592151304	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2225	0.301050768660412	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06332213920587543	0.0
2	0.0	0.0	0.0	0.2574829720273097	0.0
3	0.0	0.0	0.0	0.440142988967335	0.0
4	0.0	0.0	0.0	0.6038604856320984	0.0
5	0.0	0.0	0.0	0.9823049799885804	0.0
6	0.0	0.0	0.0	1.728099063968891	0.0
7	0.0	0.0	0.0	2.263360565461291	0.0
8	0.0	0.0	0.0	3.433873014756223	0.0
9	0.0	0.0	0.0	4.05356403518979	0.0
10	0.0	0.0	0.0	4.943456576978344	0.0
11	0.0	0.0	0.0	5.59643231160987	0.0
12	0.0	0.0	0.0	6.146171310741221	0.0
13	0.0	0.0	0.0	6.465893992244391	0.0
14	0.0	0.0	0.0	6.626228896002858	0.0
15	0.0	0.0	0.0	6.780069221381234	0.0
16	0.0	0.0	0.0	7.089914731598018	0.0
17	0.0	0.0	0.0	7.39746007863852	0.0
18	0.0	0.0	0.0	7.8599281807874135	0.0
19	0.0	0.0	0.0	8.037446656509868	0.0
20	0.0	0.0	0.0	8.246896809267763	0.0
21	0.0	0.0	0.0	8.469877333650846	0.0
22	0.0	0.0	0.0	8.701246688441545	0.0
23	1.353037162518706E-4	0.0	0.0	8.961841645942647	0.0
24	1.353037162518706E-4	0.0	0.0	9.164120701739193	0.0
25	1.353037162518706E-4	0.0	0.0	9.356116675100598	0.0
26	1.353037162518706E-4	0.0	0.0	9.52835830588923	0.0
27	1.353037162518706E-4	0.0	0.0	9.725631124184456	0.0
28	1.353037162518706E-4	0.0	0.0	9.930345646873537	0.0
29	1.353037162518706E-4	0.0	0.0	10.136548510441388	0.0
30	1.353037162518706E-4	0.0	0.0	10.434622597344259	0.0
31	1.353037162518706E-4	0.0	0.0	10.656926603146083	0.0
32	1.353037162518706E-4	0.0	0.0	10.878960001515402	0.0
33	1.353037162518706E-4	0.0	0.0	11.093145784342113	0.0
34	1.353037162518706E-4	0.0	0.0	11.300701685072482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	30	4.865151E-6	40.000004	1
TACGGGT	110	0.0	40.0	4
CGTTTTT	5980	0.0	38.46154	1
CACGACC	270	0.0	37.03704	27
GCGAGAC	275	0.0	36.363636	21
CGATCGG	55	2.1645974E-10	36.363636	10
TCGACGG	55	2.1645974E-10	36.363636	2
ACGGGTA	95	0.0	35.789474	5
CGATTGT	45	5.4380507E-8	35.555557	10
ACACGAC	300	0.0	35.333332	26
GCGATCG	120	0.0	35.000004	9
ACCGGGT	80	0.0	35.0	4
CTCGTAG	40	8.694333E-7	35.0	1
AGACACG	295	0.0	34.576275	24
CTTAACG	35	1.4003928E-5	34.285713	1
ACGCATG	35	1.4003928E-5	34.285713	1
CTATACG	35	1.4003928E-5	34.285713	1
AAACGCG	35	1.4003928E-5	34.285713	1
CGAATAT	35	1.4003928E-5	34.285713	14
TCGTACG	35	1.4003928E-5	34.285713	30
>>END_MODULE