FastQCFastQC Report
Sat 18 Jun 2016
SRR3553766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744964
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42750.5738532331763683No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29080.39035443323435765No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT9540.12805987940356847No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8980.12054273763564415No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8970.12040850296121691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG304.8652055E-640.01
GACGTAC200.001255484440.033
GCGCCTA200.001255484440.018
GCGTTCC304.8652055E-640.029
CGTTATC200.001255484440.017
AATTCGT200.001255484440.031
TCGTACA200.001255484440.034
CGTGTAA200.001255484440.011
GTATACG304.8652055E-640.01
TTACCGG451.2205419E-940.02
CTAGGCG257.79201E-540.01
TACGCAT850.037.64705716
TATACGG552.1645974E-1036.3636362
TCGATCA1600.036.2517
TATTACG455.4380507E-835.5555571
GCGCGAC1750.035.428579
TAGGGCC1750.035.428575
CTCGATC1650.035.15151616
CTTAACG408.69446E-735.01
CGTAAGG408.69446E-735.02
Produced by FastQC (version 0.11.5)