##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553766_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744964
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.759105137966397	31.0	31.0	34.0	30.0	34.0
2	31.908339194914117	33.0	31.0	34.0	30.0	34.0
3	31.881355340660757	33.0	31.0	34.0	30.0	34.0
4	35.62760079681703	37.0	35.0	37.0	33.0	37.0
5	35.43704393769363	37.0	35.0	37.0	33.0	37.0
6	35.43766544423623	37.0	35.0	37.0	33.0	37.0
7	35.72570083923519	37.0	35.0	37.0	35.0	37.0
8	35.49634210512186	37.0	35.0	37.0	33.0	37.0
9	37.23024602531129	39.0	37.0	39.0	34.0	39.0
10	36.76832437540606	39.0	37.0	39.0	32.0	39.0
11	36.749720791877195	39.0	37.0	39.0	32.0	39.0
12	36.75075439887028	39.0	35.0	39.0	32.0	39.0
13	35.07237933645116	39.0	35.0	39.0	30.0	39.0
14	36.86871848841018	39.0	36.0	41.0	30.0	41.0
15	37.508910497688476	40.0	36.0	41.0	32.0	41.0
16	37.70419241735171	40.0	36.0	41.0	33.0	41.0
17	37.75496802530055	39.0	36.0	41.0	33.0	41.0
18	37.788161575592916	39.0	37.0	41.0	33.0	41.0
19	37.75743660096327	39.0	36.0	41.0	33.0	41.0
20	37.7012540203285	39.0	36.0	41.0	33.0	41.0
21	37.56263255674099	39.0	36.0	41.0	32.0	41.0
22	37.55434490794186	39.0	36.0	41.0	32.0	41.0
23	37.47894126427586	39.0	36.0	41.0	32.0	41.0
24	37.42329696468554	39.0	36.0	41.0	32.0	41.0
25	37.18333503363921	39.0	35.0	41.0	32.0	41.0
26	37.19329256178822	39.0	35.0	41.0	32.0	41.0
27	37.15812307708829	39.0	35.0	41.0	32.0	41.0
28	37.00693053624068	39.0	35.0	41.0	31.0	41.0
29	36.94256635219957	39.0	35.0	41.0	31.0	41.0
30	36.82543451764112	39.0	35.0	41.0	31.0	41.0
31	36.77196213508304	39.0	35.0	41.0	31.0	41.0
32	36.62539934815642	39.0	35.0	40.0	30.0	41.0
33	36.49552193126111	39.0	35.0	40.0	30.0	41.0
34	36.39945957120076	39.0	35.0	40.0	30.0	41.0
35	36.29017912274956	39.0	35.0	40.0	30.0	41.0
36	36.13459576570143	38.0	35.0	40.0	30.0	41.0
37	36.091342668907494	38.0	35.0	40.0	30.0	41.0
38	35.952305077829266	38.0	35.0	40.0	29.0	41.0
39	35.84942896569498	38.0	35.0	40.0	29.0	41.0
40	35.76213481456822	38.0	35.0	40.0	28.0	41.0
41	35.65960771258745	38.0	35.0	40.0	28.0	41.0
42	35.613624282515666	38.0	34.0	40.0	28.0	41.0
43	35.52283868750705	38.0	34.0	40.0	28.0	41.0
44	35.40778346336199	38.0	34.0	40.0	27.0	41.0
45	35.340231205803235	38.0	34.0	40.0	27.0	41.0
46	34.392112907469354	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	23.0
10	34.0
11	34.0
12	23.0
13	12.0
14	18.0
15	32.0
16	63.0
17	89.0
18	188.0
19	364.0
20	687.0
21	1127.0
22	1656.0
23	2505.0
24	3416.0
25	4753.0
26	6309.0
27	8165.0
28	10376.0
29	12505.0
30	15609.0
31	19661.0
32	25711.0
33	34210.0
34	50975.0
35	65811.0
36	74976.0
37	103057.0
38	150602.0
39	151963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.68117654007442	23.22796806288626	20.513877180642286	23.576978216397034
2	24.294731020559382	28.65078044039712	23.319381876171196	23.735106662872298
3	27.84872826069448	27.397833989293442	22.811840572161877	21.941597177850205
4	25.32014969850892	28.018400889170486	21.64869711825001	25.012752294070584
5	21.646415128784746	32.77138224128951	20.44085351775388	25.141349112171863
6	20.298564762861023	40.29429610021424	21.148941425357467	18.25819771156727
7	81.14405528320832	6.974699448563958	7.338609650936152	4.542635617291574
8	80.30388045596834	7.0521528557084645	7.228268748556978	5.415697939766217
9	72.05985792602058	9.941688457428816	10.837704909230512	7.160748707320086
10	34.40797139190618	27.88658243888295	18.13792344328048	19.56752272593038
11	29.21094173678191	27.875038256882213	23.44328048066752	19.470739525668353
12	24.650989846489228	24.39768901584506	29.75915614714268	21.192164990523032
13	22.535182908167375	28.271299015791367	29.581295203526615	19.612222872514646
14	21.012424761464985	28.233310602928462	28.36835068540225	22.385913950204305
15	19.616652616770743	25.701913112579938	33.25811717076262	21.423317099886706
16	21.774877712211595	25.715470814697085	30.55409388910068	21.955557583990636
17	21.14491438512465	26.831497897884997	26.603298951358724	25.420288765631625
18	23.116821752460524	25.366729130535166	28.32378477349241	23.1926643435119
19	25.370621936093556	27.32319951031191	25.584189303107262	21.721989250487272
20	26.342480978946636	25.63358766329648	25.325384850811584	22.698546506945302
21	25.576940630688195	28.212638463066668	25.146584264474527	21.06383664177061
22	24.399299831938187	26.186768756611055	24.6207870447431	24.793144366707654
23	23.078564870248762	28.214383513834225	25.36310479432563	23.34394682159138
24	22.213825097588607	26.154552434748524	27.596904011469007	24.034718456193858
25	22.77787919953179	27.995178290494575	25.667146331903286	23.55979617807035
26	21.405598122862312	28.01679007307736	26.58410339291563	23.9935084111447
27	23.384888397291682	26.94989288072981	25.559624357687085	24.10559436429143
28	22.56189560837839	28.18404647741367	27.33890496721989	21.91515294698804
29	22.91466433277313	27.487100047787543	25.51438727240511	24.08384834703422
30	23.56556826907072	27.177823357907226	24.847509409850677	24.409098963171374
31	25.512373752288703	27.206146874211374	23.44234083794653	23.839138535553396
32	24.628304186511027	28.45036807147728	23.003661921918376	23.91766582009332
33	24.081029418871246	29.01979156039755	23.34824233117305	23.550936689558153
34	23.44328048066752	27.901348253069948	24.227613683345773	24.427757582916758
35	23.950150611304707	28.76340333224156	23.36864600168599	23.917800054767746
36	24.595013987253076	30.43059798862764	22.58270198291461	22.391686041204675
37	23.404620894432483	30.147228590911773	23.45750935615681	22.990641158498935
38	22.33597865131738	31.720324740524376	23.5191230717189	22.424573536439343
39	23.07010808575985	28.697494107097793	24.0161940711229	24.21620373601946
40	22.419741088159963	27.56804355646716	27.135942139485934	22.87627321588694
41	20.89228472785262	27.713688178220693	26.27415552966318	25.11987156426351
42	22.835868578884348	27.73744771559431	25.78527284539924	23.6414108601221
43	23.100445122180403	26.501817537491746	27.097819491948606	23.299917848379252
44	23.50892123646243	27.56321110818778	25.871451506381515	23.056416148968275
45	22.18577005063332	27.08359061645932	26.540745593075638	24.18989373983172
46	22.716265483969693	27.773019904317525	25.680167095322727	23.830547516390055
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4747.0
1	4210.5
2	3674.0
3	4716.5
4	5759.0
5	4059.5
6	2360.0
7	2360.0
8	2308.0
9	2256.0
10	2306.0
11	2356.0
12	2289.5
13	2223.0
14	2291.0
15	2359.0
16	2204.5
17	2050.0
18	2190.0
19	2330.0
20	2330.0
21	2337.5
22	2345.0
23	2984.0
24	3623.0
25	4741.0
26	5859.0
27	6305.0
28	6751.0
29	8345.5
30	9940.0
31	9940.0
32	11533.5
33	13127.0
34	14397.0
35	15667.0
36	17939.0
37	20211.0
38	23689.0
39	27167.0
40	29657.5
41	32148.0
42	36844.0
43	41540.0
44	41540.0
45	48571.0
46	55602.0
47	61607.5
48	67613.0
49	68315.5
50	69018.0
51	64826.5
52	60635.0
53	54456.5
54	48278.0
55	47032.0
56	45786.0
57	45786.0
58	44415.0
59	43044.0
60	40421.5
61	37799.0
62	34533.0
63	31267.0
64	27865.0
65	24463.0
66	20915.0
67	17367.0
68	15127.5
69	12888.0
70	12888.0
71	10842.0
72	8796.0
73	7354.0
74	5912.0
75	4873.0
76	3834.0
77	2994.0
78	2154.0
79	1553.0
80	952.0
81	952.0
82	827.0
83	702.0
84	450.0
85	198.0
86	141.0
87	84.0
88	61.0
89	38.0
90	34.5
91	31.0
92	19.0
93	7.0
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	744964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.362446793026983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66185551433551	22.669166155226797
2	9.17942828794639	5.57419805966358
3	3.603069268132955	3.281939968358326
4	1.9473700509025442	2.3650767822745102
5	1.1927397743258425	1.8107248967947704
6	0.8916947786365629	1.6244421163183569
7	0.7046973594327222	1.4977435256673848
8	0.5643409660965238	1.3707818044984919
9	0.4980405266097097	1.3609556090962605
>10	6.151355330429347	43.01919008354515
>50	0.5463764107284271	10.385914507871618
>100	0.05281786585255102	2.965594470132352
>500	0.005326171346475733	1.1062694267616322
>1k	8.87695224412622E-4	0.9680025937908154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4275	0.5738532331763683	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2908	0.39035443323435765	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT	954	0.12805987940356847	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	898	0.12054273763564415	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	897	0.12040850296121691	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08054080465633238	0.0
2	0.0	0.0	0.0	0.42082570432933675	0.0
3	0.0	0.0	0.0	0.619895726504905	0.0
4	0.0	0.0	0.0	0.8360135523327302	0.0
5	0.0	0.0	0.0	1.445573208906739	0.0
6	0.0	0.0	0.0	2.3786384308503496	0.0
7	0.0	0.0	0.0	3.0731686363367894	0.0
8	0.0	0.0	0.0	4.615390810831127	0.0
9	0.0	0.0	0.0	5.3856293726945195	0.0
10	0.0	0.0	0.0	6.583539607283036	0.0
11	0.0	0.0	0.0	7.489489424992349	0.0
12	0.0	0.0	0.0	8.341208434233064	0.0
13	0.0	0.0	0.0	8.835326270799662	0.0
14	0.0	0.0	0.0	9.03493323167294	1.3423467442722064E-4
15	0.0	0.0	0.0	9.305013396620508	1.3423467442722064E-4
16	0.0	0.0	0.0	9.805037558861905	1.3423467442722064E-4
17	0.0	0.0	0.0	10.31861942322045	1.3423467442722064E-4
18	0.0	0.0	0.0	11.11838961345783	1.3423467442722064E-4
19	0.0	0.0	0.0	11.399745491057287	1.3423467442722064E-4
20	0.0	0.0	0.0	11.742849318893262	1.3423467442722064E-4
21	0.0	0.0	0.0	12.089174778915492	1.3423467442722064E-4
22	0.0	0.0	0.0	12.4432858500545	1.3423467442722064E-4
23	0.0	0.0	0.0	12.781020290913386	1.3423467442722064E-4
24	0.0	0.0	0.0	13.050966221186528	1.3423467442722064E-4
25	0.0	0.0	0.0	13.315811233831434	1.3423467442722064E-4
26	0.0	0.0	0.0	13.535284926519939	1.3423467442722064E-4
27	0.0	0.0	0.0	13.788183053140823	1.3423467442722064E-4
28	0.0	0.0	0.0	14.035711792784618	1.3423467442722064E-4
29	0.0	0.0	0.0	14.287536042010084	1.3423467442722064E-4
30	0.0	0.0	0.0	14.649566958940298	1.3423467442722064E-4
31	0.0	0.0	0.0	14.910384931352388	1.3423467442722064E-4
32	0.0	0.0	0.0	15.17106866909005	1.3423467442722064E-4
33	0.0	0.0	0.0	15.436047916409384	1.3423467442722064E-4
34	0.0	0.0	0.0	15.682368543983333	1.3423467442722064E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	30	4.8652055E-6	40.0	1
GACGTAC	20	0.0012554844	40.0	33
GCGCCTA	20	0.0012554844	40.0	18
GCGTTCC	30	4.8652055E-6	40.0	29
CGTTATC	20	0.0012554844	40.0	17
AATTCGT	20	0.0012554844	40.0	31
TCGTACA	20	0.0012554844	40.0	34
CGTGTAA	20	0.0012554844	40.0	11
GTATACG	30	4.8652055E-6	40.0	1
TTACCGG	45	1.2205419E-9	40.0	2
CTAGGCG	25	7.79201E-5	40.0	1
TACGCAT	85	0.0	37.647057	16
TATACGG	55	2.1645974E-10	36.363636	2
TCGATCA	160	0.0	36.25	17
TATTACG	45	5.4380507E-8	35.555557	1
GCGCGAC	175	0.0	35.42857	9
TAGGGCC	175	0.0	35.42857	5
CTCGATC	165	0.0	35.151516	16
CTTAACG	40	8.69446E-7	35.0	1
CGTAAGG	40	8.69446E-7	35.0	2
>>END_MODULE