FastQCFastQC Report
Sat 18 Jun 2016
SRR3553764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842887
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101691.2064487885090172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38270.4540347638532805No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG200.001255578440.01
TACGCAT257.792883E-540.016
CATAACG200.001255578440.01
CGGTCTA257.792883E-540.031
CCACGCG200.001255578440.01
TAATGCG353.0550837E-740.01
CGTTTTT39800.037.688441
CGTAAGG850.037.647062
TAGCATA3500.036.030
CTCGTAG455.439506E-835.5555571
CATATGC3500.035.4285733
GCGATAT1300.035.3846139
TAGGGTA2400.035.05
ATATGCT3500.034.85714334
TACGACG351.4006255E-534.2857131
CGTTCGA653.45608E-1133.84615314
CTTAACG605.511538E-1033.3333321
CATTGCG302.277748E-433.3333321
TCGTAAG302.277748E-433.3333321
ACGTCGC302.277748E-433.33333220
Produced by FastQC (version 0.11.5)