##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553764_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	842887
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85211422171655	31.0	31.0	34.0	30.0	34.0
2	32.008167168315566	33.0	31.0	34.0	30.0	34.0
3	31.888601912237345	33.0	31.0	34.0	30.0	34.0
4	35.682182783694614	37.0	35.0	37.0	33.0	37.0
5	35.506740523937374	37.0	35.0	37.0	33.0	37.0
6	35.505961060023466	37.0	35.0	37.0	33.0	37.0
7	35.763435668126334	37.0	35.0	37.0	35.0	37.0
8	35.551474871483364	37.0	35.0	37.0	35.0	37.0
9	37.32487391548333	39.0	37.0	39.0	34.0	39.0
10	36.8788580201142	39.0	37.0	39.0	32.0	39.0
11	36.84264201488456	39.0	37.0	39.0	32.0	39.0
12	36.84105461348911	39.0	37.0	39.0	33.0	39.0
13	35.14751087630964	39.0	35.0	39.0	30.0	39.0
14	36.98350194035499	40.0	36.0	41.0	31.0	41.0
15	37.630802230904024	40.0	36.0	41.0	32.0	41.0
16	37.81604414352102	40.0	37.0	41.0	33.0	41.0
17	37.83976262535785	40.0	37.0	41.0	33.0	41.0
18	37.84062276438004	39.0	37.0	41.0	33.0	41.0
19	37.75017529040073	39.0	37.0	41.0	33.0	41.0
20	37.65409123642908	39.0	36.0	41.0	33.0	41.0
21	37.506407145916356	39.0	35.0	41.0	33.0	41.0
22	37.477393766898764	39.0	35.0	41.0	33.0	41.0
23	37.38823116265881	39.0	35.0	41.0	32.0	41.0
24	37.30131085186982	39.0	35.0	41.0	32.0	41.0
25	37.084972244203556	39.0	35.0	41.0	32.0	41.0
26	37.050551260133325	39.0	35.0	41.0	32.0	41.0
27	37.01241328908857	39.0	35.0	41.0	32.0	41.0
28	36.85381314458522	39.0	35.0	41.0	31.0	41.0
29	36.75410464273384	39.0	35.0	40.0	31.0	41.0
30	36.586939886366736	39.0	35.0	40.0	31.0	41.0
31	36.48135277919816	39.0	35.0	40.0	30.0	41.0
32	36.26173496565969	39.0	35.0	40.0	30.0	41.0
33	36.069199074134495	39.0	35.0	40.0	29.0	41.0
34	35.857547927539514	38.0	35.0	40.0	28.0	41.0
35	35.732221519610576	38.0	35.0	40.0	27.0	41.0
36	35.54106185052089	38.0	35.0	40.0	27.0	41.0
37	35.486421074236524	38.0	35.0	40.0	27.0	41.0
38	35.332248569499825	38.0	34.0	40.0	26.0	41.0
39	35.18918194253797	38.0	34.0	40.0	25.0	41.0
40	35.06553903429523	38.0	34.0	40.0	25.0	41.0
41	34.930496021412125	38.0	34.0	40.0	24.0	41.0
42	34.827910502831344	38.0	34.0	40.0	24.0	41.0
43	34.716756813190855	38.0	34.0	40.0	24.0	41.0
44	34.57868848374693	37.0	34.0	40.0	23.0	41.0
45	34.47581941588849	37.0	33.0	40.0	23.0	41.0
46	33.49353353415108	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	16.0
10	39.0
11	28.0
12	20.0
13	19.0
14	20.0
15	43.0
16	76.0
17	135.0
18	263.0
19	505.0
20	962.0
21	1576.0
22	2361.0
23	3454.0
24	4818.0
25	6631.0
26	9192.0
27	12274.0
28	15401.0
29	17208.0
30	19802.0
31	23477.0
32	29111.0
33	37654.0
34	55684.0
35	72210.0
36	83896.0
37	114353.0
38	164126.0
39	167529.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.182827591361594	20.55518711286329	20.834346715514656	27.42763858026046
2	28.052633389766363	26.841083087056745	22.191349492873897	22.914934030302994
3	27.08726080720191	25.963504004688648	25.345864866820822	21.60337032128862
4	24.436134381002436	27.280643787364145	24.193871776406564	24.08935005522686
5	21.252314960368356	32.13087875361703	22.92489977897393	23.691906507040684
6	20.276502069672446	38.607073071479334	22.975914920979918	18.140509937868302
7	79.96208269910439	6.555089828173884	9.359617599986713	4.123209872735017
8	79.53319958665871	6.342012630400042	9.570559280188212	4.5542285027530385
9	72.37411420510698	8.449768474303198	12.660653207369435	6.515464113220395
10	35.68924422846716	26.175394803811187	19.311129487108	18.824231480613655
11	29.138544075303095	27.192019808111883	24.687057695752813	18.98237842083221
12	23.943067101521322	24.662261963940598	29.990734226533334	21.40393670800475
13	22.55130284367893	26.50592546806393	30.63696557189754	20.305806116359605
14	21.819057596095327	26.407573019870988	29.679423220431683	22.093946163602002
15	21.36276867480457	24.79276581558382	32.02315375607881	21.8213117535328
16	22.630316993855644	24.337307373348978	31.096576409411934	21.93579922338344
17	22.704704189292276	24.625246326019976	28.78440407788944	23.8856454067983
18	23.49093057551012	24.257937303576874	28.924043199147693	23.327088921765313
19	25.738444180536657	25.780324052927618	26.851997954648727	21.629233811887
20	27.126293322829753	24.194939535192734	26.63239556429272	22.04637157768479
21	26.873946329697812	25.981537264188436	26.268764377668656	20.875752028445092
22	25.311340666067935	24.457608196591003	26.15439554768314	24.076655589657925
23	24.74969954454156	26.17693712205788	26.18797062951499	22.885392703885575
24	22.998693775084917	25.55823022540388	28.733270296018325	22.709805703492876
25	23.841748656700126	26.103973605002807	27.189647010809274	22.86463072748779
26	22.780515063110478	26.269950776319956	27.729695676881956	23.21983848368761
27	24.4045761768778	24.920066390868527	26.9677904630158	23.70756696923787
28	23.51264167082895	26.88865767297396	27.587209198860585	22.01149145733651
29	24.16207629255167	25.769765104931032	26.650903383253034	23.417255219264266
30	24.329121222654994	25.12602519673456	27.178969422947556	23.36588415766289
31	25.33376360057754	25.314069382965926	24.98603015588092	24.366136860575615
32	25.630007343807655	25.73642730282944	25.642701809376582	22.990863543986322
33	23.957066605606684	26.146209396989157	24.46650618647577	25.43021781092839
34	23.503980960674443	27.841454429834606	25.102059943978254	23.552504665512696
35	23.99704824015556	25.750545446779938	25.1760912198195	25.076315093245
36	23.76095490854646	28.654730705302136	24.62311080844763	22.961203577703774
37	23.70305865436292	27.765880835746664	24.499369429116832	24.03169108077358
38	23.084114477978662	27.655545761175578	26.260222307379284	23.00011745346648
39	23.358172566429428	26.79481353965597	25.453945783954435	24.393068109960172
40	23.91435625415981	25.731444428493973	27.883927501551213	22.470271815795
41	21.90483421858446	26.661699610979884	27.322760939485363	24.110705230950295
42	23.466965322753822	25.975130711471405	27.076701859205325	23.481202106569445
43	22.99466001967049	25.235767071979993	27.97065324296139	23.798919665388123
44	24.0914855727992	25.615651920126897	26.5941935277208	23.698668979353105
45	23.315343575117424	24.671041313960234	27.83481059738731	24.17880451353503
46	23.340139306929636	26.08333026847015	26.550889976948273	24.025640447651938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4177.0
1	3651.0
2	3125.0
3	10130.5
4	17136.0
5	11524.5
6	5913.0
7	5913.0
8	5566.0
9	5219.0
10	5097.0
11	4975.0
12	4951.0
13	4927.0
14	4548.0
15	4169.0
16	3741.5
17	3314.0
18	3163.0
19	3012.0
20	3012.0
21	2929.0
22	2846.0
23	3323.0
24	3800.0
25	4474.5
26	5149.0
27	5990.0
28	6831.0
29	8643.5
30	10456.0
31	10456.0
32	11628.5
33	12801.0
34	14440.5
35	16080.0
36	18196.0
37	20312.0
38	23336.0
39	26360.0
40	29004.5
41	31649.0
42	36911.0
43	42173.0
44	42173.0
45	46390.0
46	50607.0
47	56850.5
48	63094.0
49	65655.0
50	68216.0
51	65092.5
52	61969.0
53	58196.5
54	54424.0
55	52849.5
56	51275.0
57	51275.0
58	49902.0
59	48529.0
60	47437.0
61	46345.0
62	43554.5
63	40764.0
64	36742.5
65	32721.0
66	30276.0
67	27831.0
68	24257.0
69	20683.0
70	20683.0
71	17607.5
72	14532.0
73	12660.0
74	10788.0
75	8911.5
76	7035.0
77	5910.5
78	4786.0
79	3710.0
80	2634.0
81	2634.0
82	1886.5
83	1139.0
84	855.0
85	571.0
86	425.0
87	279.0
88	231.5
89	184.0
90	112.0
91	40.0
92	26.5
93	13.0
94	13.0
95	8.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	842887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.798223192796787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3208656247816	23.31473249825867
2	9.007916286488486	5.728714651595802
3	3.6239761801126193	3.4570801026180065
4	2.0674588619588508	2.6296607333797275
5	1.393710133592966	2.215875294702588
6	1.076983423637131	2.0547695567853546
7	0.8780715989887053	1.9544781678729168
8	0.7167395811981839	1.823283613924124
9	0.6091106483544644	1.7431772710935975
>10	7.092418208556689	47.020704898273834
>50	0.17288222020187877	3.392005484891796
>100	0.034278367811315796	2.012692428440073
>500	0.004843682408120622	0.994616961193445
>1k	3.7259095447081706E-4	0.45341263972451634
>5k	0.0	0.0
>10k+	3.7259095447081706E-4	1.2047956972455203
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10169	1.2064487885090172	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3827	0.4540347638532805	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1863986513020132E-4	0.0	0.0	0.0450831487494765	0.0
2	1.1863986513020132E-4	0.0	0.0	0.18353587135642144	0.0
3	1.1863986513020132E-4	0.0	0.0	0.37110549812726973	0.0
4	1.1863986513020132E-4	0.0	0.0	0.5444383410824939	0.0
5	1.1863986513020132E-4	0.0	0.0	0.8826805965686978	0.0
6	1.1863986513020132E-4	0.0	0.0	1.5483688798142574	0.0
7	1.1863986513020132E-4	0.0	0.0	2.067774209354279	0.0
8	1.1863986513020132E-4	0.0	0.0	3.2328176849328556	0.0
9	1.1863986513020132E-4	0.0	0.0	3.850456822800684	0.0
10	1.1863986513020132E-4	0.0	0.0	4.741560849793626	0.0
11	1.1863986513020132E-4	0.0	0.0	5.311032202418592	0.0
12	1.1863986513020132E-4	0.0	0.0	5.858673819859602	0.0
13	1.1863986513020132E-4	0.0	0.0	6.2199322091810645	0.0
14	1.1863986513020132E-4	0.0	0.0	6.391604094024466	0.0
15	1.1863986513020132E-4	0.0	0.0	6.5370565686740925	0.0
16	1.1863986513020132E-4	0.0	0.0	6.793437317220458	0.0
17	1.1863986513020132E-4	0.0	0.0	7.044953831296485	0.0
18	1.1863986513020132E-4	0.0	0.0	7.398737909114745	0.0
19	1.1863986513020132E-4	0.0	0.0	7.538614310103252	0.0
20	1.1863986513020132E-4	0.0	0.0	7.711828513193346	0.0
21	1.1863986513020132E-4	0.0	0.0	7.877449764915108	0.0
22	1.1863986513020132E-4	0.0	0.0	8.037969502436269	0.0
23	1.1863986513020132E-4	0.0	0.0	8.225064569746596	0.0
24	1.1863986513020132E-4	0.0	0.0	8.378228635629688	0.0
25	1.1863986513020132E-4	0.0	0.0	8.522376071762881	0.0
26	1.1863986513020132E-4	0.0	0.0	8.655727280169227	0.0
27	1.1863986513020132E-4	0.0	0.0	8.81636565755552	0.0
28	1.1863986513020132E-4	0.0	0.0	8.961343572744626	0.0
29	1.1863986513020132E-4	0.0	0.0	9.109287484561987	0.0
30	1.1863986513020132E-4	0.0	0.0	9.315246290428018	0.0
31	1.1863986513020132E-4	0.0	0.0	9.480867542149777	0.0
32	1.1863986513020132E-4	0.0	0.0	9.650997108746488	0.0
33	1.1863986513020132E-4	0.0	0.0	9.820889395612936	0.0
34	1.1863986513020132E-4	0.0	0.0	9.981883692594618	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	0.0012555784	40.0	1
TACGCAT	25	7.792883E-5	40.0	16
CATAACG	20	0.0012555784	40.0	1
CGGTCTA	25	7.792883E-5	40.0	31
CCACGCG	20	0.0012555784	40.0	1
TAATGCG	35	3.0550837E-7	40.0	1
CGTTTTT	3980	0.0	37.68844	1
CGTAAGG	85	0.0	37.64706	2
TAGCATA	350	0.0	36.0	30
CTCGTAG	45	5.439506E-8	35.555557	1
CATATGC	350	0.0	35.42857	33
GCGATAT	130	0.0	35.384613	9
TAGGGTA	240	0.0	35.0	5
ATATGCT	350	0.0	34.857143	34
TACGACG	35	1.4006255E-5	34.285713	1
CGTTCGA	65	3.45608E-11	33.846153	14
CTTAACG	60	5.511538E-10	33.333332	1
CATTGCG	30	2.277748E-4	33.333332	1
TCGTAAG	30	2.277748E-4	33.333332	1
ACGTCGC	30	2.277748E-4	33.333332	20
>>END_MODULE