FastQCFastQC Report
Sat 18 Jun 2016
SRR3553763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442650
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29210.6598893030611093No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.24172596859821527No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10250.2315599231898791No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6700.1513611205241161No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT6290.14209872359652095No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA5420.12244436914040438No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT4710.10640460860725177No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAG200.001254932540.09
CTATACG353.0507E-740.01
CGTTGAT451.2187229E-940.025
ATTACGG257.78688E-540.02
GCCGATG401.9237632E-840.09
ATATCAT304.8607253E-640.028
CTAGTAC257.78688E-540.023
TTGACGT304.8607253E-640.018
TGTTCGA304.8607253E-640.038
ACGTCTA200.001254932540.028
TTACGGG900.040.03
TACTACG257.78688E-540.01
TTCGACG200.001254932540.025
GTGCGAT200.001254932540.030
TCGAACG200.001254932540.01
GCGTAAG257.78688E-540.01
AAGTCGA304.8607253E-640.033
TAATCGT507.8216544E-1140.021
CGTAAGG650.039.9999962
GCGAGAC1650.037.5757621
Produced by FastQC (version 0.11.5)