##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553763_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	442650
Sequences flagged as poor quality	0
Sequence length	46
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80798825256975	31.0	31.0	34.0	30.0	34.0
2	31.951998192703037	33.0	31.0	34.0	30.0	34.0
3	31.924190669829436	33.0	31.0	34.0	30.0	34.0
4	35.6697503671072	37.0	35.0	37.0	33.0	37.0
5	35.47202982039986	37.0	35.0	37.0	33.0	37.0
6	35.46679091833277	37.0	35.0	37.0	33.0	37.0
7	35.746897097029255	37.0	35.0	37.0	35.0	37.0
8	35.57426183214729	37.0	35.0	37.0	35.0	37.0
9	37.355359765051396	39.0	37.0	39.0	35.0	39.0
10	36.77549757144471	39.0	37.0	39.0	32.0	39.0
11	36.73470010166045	39.0	37.0	39.0	32.0	39.0
12	36.686639557212246	39.0	35.0	39.0	32.0	39.0
13	35.004348808313566	39.0	35.0	39.0	30.0	39.0
14	36.75447644866147	39.0	36.0	41.0	30.0	41.0
15	37.39174743024963	39.0	36.0	41.0	32.0	41.0
16	37.612032079521065	39.0	36.0	41.0	33.0	41.0
17	37.66101208629843	39.0	36.0	41.0	33.0	41.0
18	37.70417937422343	39.0	36.0	41.0	33.0	41.0
19	37.6628013102903	39.0	36.0	41.0	33.0	41.0
20	37.629908505591324	39.0	36.0	41.0	33.0	41.0
21	37.49648480740992	39.0	36.0	41.0	32.0	41.0
22	37.46989269174291	39.0	35.0	41.0	32.0	41.0
23	37.3973206822546	39.0	35.0	41.0	32.0	41.0
24	37.33059076019428	39.0	35.0	41.0	32.0	41.0
25	37.146736699423926	39.0	35.0	41.0	32.0	41.0
26	37.09443804360104	39.0	35.0	41.0	32.0	41.0
27	37.0637817688919	39.0	35.0	41.0	32.0	41.0
28	36.93898339545917	39.0	35.0	41.0	31.0	41.0
29	36.86123348017621	39.0	35.0	40.0	31.0	41.0
30	36.71696374110471	39.0	35.0	40.0	31.0	41.0
31	36.651733875522424	39.0	35.0	40.0	31.0	41.0
32	36.548467186264546	39.0	35.0	40.0	30.0	41.0
33	36.432736925336044	39.0	35.0	40.0	30.0	41.0
34	36.33912346097368	39.0	35.0	40.0	30.0	41.0
35	36.23189427312775	38.0	35.0	40.0	30.0	41.0
36	36.091706766068	38.0	35.0	40.0	30.0	41.0
37	36.009802326894835	38.0	35.0	40.0	30.0	41.0
38	35.9015881622049	38.0	35.0	40.0	29.0	41.0
39	35.81599457810911	38.0	35.0	40.0	29.0	41.0
40	35.70385180164916	38.0	35.0	40.0	28.0	41.0
41	35.59373319778606	38.0	34.0	40.0	28.0	41.0
42	35.548187055235516	38.0	34.0	40.0	28.0	41.0
43	35.46584660567039	38.0	34.0	40.0	28.0	41.0
44	35.32472834067548	38.0	34.0	40.0	27.0	41.0
45	35.241104710267706	38.0	34.0	40.0	27.0	41.0
46	34.261140856206936	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	18.0
10	17.0
11	20.0
12	10.0
13	11.0
14	7.0
15	24.0
16	32.0
17	72.0
18	140.0
19	263.0
20	465.0
21	694.0
22	1069.0
23	1524.0
24	2115.0
25	2790.0
26	3727.0
27	4817.0
28	6135.0
29	7582.0
30	9439.0
31	11697.0
32	15280.0
33	20988.0
34	31680.0
35	40753.0
36	44108.0
37	60783.0
38	87786.0
39	88600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.048571105839834	21.348469445385746	20.64226815768666	23.960691291087766
2	23.716480289167514	28.459279340336607	24.068677284536317	23.75556308595956
3	27.915734779170904	27.871004179374225	23.150570428103467	21.062690613351407
4	25.59923189879137	28.80718400542189	21.151699988704394	24.441884107082345
5	22.063029481531686	33.41059527843669	19.65774313791935	24.86863210211228
6	19.83937648254829	41.371964305885015	20.620128769908504	18.168530441658195
7	83.09477013441771	6.786626002485033	6.332090816672315	3.7865130464249406
8	83.53507285665877	5.798486388794759	6.36417033773862	4.302270416807861
9	77.67174968937084	7.780639331300124	9.048909974020106	5.498701005308935
10	36.7947588388117	27.926126736699423	16.665763018185924	18.613351406302947
11	30.349034225686207	28.089235287473173	22.314017847057492	19.247712639783124
12	25.534395120298203	24.849429571896533	28.61696600022591	20.999209307579353
13	22.804021235739295	28.66869987574833	28.525923415791254	20.001355472721112
14	21.137015700892352	28.975714447080087	27.709928837682142	22.17734101434542
15	20.550773749011633	27.295380097142214	31.294702360781656	20.859143793064497
16	22.514175985541623	25.702021913475658	29.33739975149667	22.44640234948605
17	21.76143680108438	26.023720772619452	26.408675025415114	25.80616740088106
18	23.26849655484017	26.038179148311308	27.377838021009826	23.315486275838698
19	24.889190105049135	27.365638766519822	25.7090251892014	22.03614593922964
20	27.06788659211567	25.547949847509322	24.129673556986333	23.254490003388682
21	26.648367784931658	27.446741217666325	24.785270529763924	21.11962046763809
22	24.60047441545239	25.134078843329945	24.793177453970404	25.47226928724726
23	23.315712187958884	27.774539704055123	25.062238789111035	23.847509318874955
24	22.535411724839037	26.470349034225688	27.865808200609965	23.128431040325314
25	23.856093979441997	26.896871117135433	25.798712300914943	23.448322602507623
26	22.473285891788095	27.5788998079747	25.728001807296963	24.219812492940246
27	23.33401106969389	27.02744832260251	25.67333107421213	23.96520953349147
28	22.826160623517453	27.682141646899357	27.222410482322378	22.269287247260817
29	23.061561052750477	27.25946007003276	25.607138822997854	24.07184005421891
30	24.169885914379307	26.19496215971987	26.456568394894386	23.17858353100644
31	25.671071953010276	26.86049926578561	24.56229526714108	22.90613351406303
32	25.968598215294254	27.326104145487406	24.27583869874619	22.429458940472156
33	24.30565909861064	26.307918219812493	24.432847622274938	24.953575059301933
34	23.926578560939795	26.956060092623968	25.09838472834068	24.01897661809556
35	24.97164802891675	26.626454309273694	24.753416920817802	23.648480740991754
36	25.302609284988144	28.10979329041003	23.961369027448324	22.626228397153508
37	24.374336383146954	28.006777363605558	24.88806054444821	22.730825708799276
38	23.7090251892014	28.87224669603524	24.651530554614254	22.767197560149103
39	23.800067773636055	26.799051169095222	24.607477691178133	24.79340336609059
40	24.124251666101888	26.770812154072065	26.479385519033094	22.62555066079295
41	21.33920704845815	26.838133965887266	26.570654015587934	25.252004970066643
42	23.909409239805715	26.316502880379534	25.452389020670957	24.321698859143794
43	23.53213599909635	25.17225799164125	26.66418163334463	24.631424375917767
44	23.94871794871795	25.93471139726646	26.239240935276175	23.87732971873941
45	24.083135660228173	25.637636959222863	25.706540155879367	24.572687224669604
46	23.709928837682142	26.73172935728002	26.00090364848074	23.5574381565571
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3303.0
1	3132.0
2	2961.0
3	2807.0
4	2653.0
5	1953.0
6	1253.0
7	1253.0
8	1236.5
9	1220.0
10	1279.0
11	1338.0
12	1384.0
13	1430.0
14	1336.5
15	1243.0
16	1237.5
17	1232.0
18	1255.0
19	1278.0
20	1278.0
21	1212.5
22	1147.0
23	1465.0
24	1783.0
25	2211.5
26	2640.0
27	2996.0
28	3352.0
29	4507.5
30	5663.0
31	5663.0
32	6861.5
33	8060.0
34	9231.5
35	10403.0
36	11278.5
37	12154.0
38	13192.0
39	14230.0
40	16132.0
41	18034.0
42	20492.5
43	22951.0
44	22951.0
45	25571.0
46	28191.0
47	30307.0
48	32423.0
49	35138.5
50	37854.0
51	39354.0
52	40854.0
53	38099.0
54	35344.0
55	31862.5
56	28381.0
57	28381.0
58	26737.0
59	25093.0
60	23383.0
61	21673.0
62	20465.0
63	19257.0
64	17592.5
65	15928.0
66	14161.0
67	12394.0
68	11213.5
69	10033.0
70	10033.0
71	8121.0
72	6209.0
73	5243.0
74	4277.0
75	3628.5
76	2980.0
77	2514.5
78	2049.0
79	1426.0
80	803.0
81	803.0
82	570.5
83	338.0
84	246.0
85	154.0
86	95.0
87	36.0
88	36.5
89	37.0
90	24.0
91	11.0
92	6.5
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	442650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.715484081535216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90446158398281	23.007267974187897
2	8.470498945422138	5.203509510415491
3	3.2015203537550634	2.950087423874239
4	1.774305763770909	2.1799464177152617
5	1.1349679132220736	1.7430544435812922
6	0.8810133463364008	1.6236450849009474
7	0.7777752040406087	1.6722819329105485
8	0.5860844202803263	1.4401493345244927
9	0.6014796215377392	1.6627263966639227
>10	7.363857305322481	50.07641391542157
>50	0.26133805696598117	4.8990334846160435
>100	0.03828050411896062	1.9914053308877273
>500	0.002208490622247728	0.41627991531330827
>1k	0.002208490622247728	1.1341988349872645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2921	0.6598893030611093	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1070	0.24172596859821527	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1025	0.2315599231898791	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	670	0.1513611205241161	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT	629	0.14209872359652095	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	542	0.12244436914040438	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT	471	0.10640460860725177	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09601265107873037	0.0
2	0.0	0.0	0.0	0.45679430701457135	0.0
3	0.0	0.0	0.0	0.6083813396588726	0.0
4	0.0	0.0	0.0	0.7875296509657743	0.0
5	0.0	0.0	0.0	1.328363266689258	0.0
6	0.0	0.0	0.0	2.095334914718175	0.0
7	0.0	0.0	0.0	2.5814977973568283	0.0
8	0.0	0.0	0.0	3.5949395685078502	0.0
9	0.0	0.0	0.0	4.044730599796679	0.0
10	0.0	0.0	0.0	4.838811702247826	0.0
11	0.0	0.0	0.0	5.607364735118039	0.0
12	0.0	0.0	0.0	6.296622613803231	0.0
13	0.0	0.0	0.0	6.620806506269061	0.0
14	0.0	0.0	0.0	6.764712526827064	0.0
15	0.0	0.0	0.0	6.946797695696374	0.0
16	0.0	0.0	0.0	7.367446063481306	0.0
17	0.0	0.0	0.0	7.842539252230882	0.0
18	0.0	0.0	0.0	8.490907037162543	0.0
19	0.0	0.0	0.0	8.735569863323168	0.0
20	0.0	0.0	0.0	9.007793968146391	0.0
21	0.0	0.0	0.0	9.311871681915735	0.0
22	0.0	0.0	0.0	9.606009262396928	0.0
23	0.0	0.0	0.0	9.934033660905907	0.0
24	0.0	0.0	0.0	10.179600135547272	0.0
25	0.0	0.0	0.0	10.417937422342709	0.0
26	0.0	0.0	0.0	10.622839715350729	0.0
27	0.0	0.0	0.0	10.843329944651531	0.0
28	0.0	0.0	0.0	11.057494634587146	0.0
29	0.0	0.0	0.0	11.291765503219247	0.0
30	0.0	0.0	0.0	11.579125720094883	0.0
31	0.0	0.0	0.0	11.846831582514401	0.0
32	0.0	0.0	0.0	12.0964644753191	0.0
33	0.0	0.0	0.0	12.329605783350276	0.0
34	0.0	0.0	0.0	12.558228848977748	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAG	20	0.0012549325	40.0	9
CTATACG	35	3.0507E-7	40.0	1
CGTTGAT	45	1.2187229E-9	40.0	25
ATTACGG	25	7.78688E-5	40.0	2
GCCGATG	40	1.9237632E-8	40.0	9
ATATCAT	30	4.8607253E-6	40.0	28
CTAGTAC	25	7.78688E-5	40.0	23
TTGACGT	30	4.8607253E-6	40.0	18
TGTTCGA	30	4.8607253E-6	40.0	38
ACGTCTA	20	0.0012549325	40.0	28
TTACGGG	90	0.0	40.0	3
TACTACG	25	7.78688E-5	40.0	1
TTCGACG	20	0.0012549325	40.0	25
GTGCGAT	20	0.0012549325	40.0	30
TCGAACG	20	0.0012549325	40.0	1
GCGTAAG	25	7.78688E-5	40.0	1
AAGTCGA	30	4.8607253E-6	40.0	33
TAATCGT	50	7.8216544E-11	40.0	21
CGTAAGG	65	0.0	39.999996	2
GCGAGAC	165	0.0	37.57576	21
>>END_MODULE