FastQCFastQC Report
Sat 18 Jun 2016
SRR3553762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498808
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40100.8039165370242659No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16320.3271799971131177No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.24759025516832128No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT11630.23315584353097782No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT11430.2291462847428269No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10870.21791952013600424No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA9030.18103157928501548No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCG8950.17942775576975512No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT8350.16739907940530224No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTA7450.14935606485862296No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCG7230.14494555019165692No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC6610.13251591794838896No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGT5290.10605282994659267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT200.001255085640.024
GTCATAC257.7883014E-540.039
CGTGGAA200.001255085640.010
TACCCGT200.001255085640.035
CTCCGTA200.001255085640.014
GGTCGTT200.001255085640.08
GGTGTAC257.7883014E-540.08
TTGTGCG257.7883014E-540.01
CAACGCG257.7883014E-540.01
ACGCATT1150.040.017
GACACGC200.001255085640.028
ACGCAAT200.001255085640.031
GTTTACG401.9244908E-840.01
GACGTTT304.8619677E-640.028
GACGTAG257.7883014E-540.01
ATTACGC200.001255085640.014
ATTACCG200.001255085640.020
CGCGATG200.001255085640.040
GCGCAAT304.8619677E-640.019
AGTATAT200.001255085640.028
Produced by FastQC (version 0.11.5)