##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553762_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498808
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83935101281455	31.0	31.0	34.0	30.0	34.0
2	31.990194623983577	33.0	31.0	34.0	30.0	34.0
3	31.96442518965213	33.0	31.0	34.0	30.0	34.0
4	35.71073840034643	37.0	35.0	37.0	33.0	37.0
5	35.501818334910425	37.0	35.0	37.0	33.0	37.0
6	35.51941027409344	37.0	35.0	37.0	33.0	37.0
7	35.79956616573912	37.0	35.0	37.0	35.0	37.0
8	35.57073663614056	37.0	35.0	37.0	35.0	37.0
9	37.37612267646068	39.0	37.0	39.0	34.0	39.0
10	36.86899368093535	39.0	37.0	39.0	32.0	39.0
11	36.836732770925884	39.0	37.0	39.0	32.0	39.0
12	36.79484290548668	39.0	35.0	39.0	33.0	39.0
13	34.97856289393915	39.0	35.0	39.0	30.0	39.0
14	36.78675161585219	39.0	36.0	41.0	30.0	41.0
15	37.43653469872175	39.0	36.0	41.0	32.0	41.0
16	37.69506303026415	39.0	36.0	41.0	33.0	41.0
17	37.75687238376289	39.0	36.0	41.0	33.0	41.0
18	37.77060512261231	39.0	36.0	41.0	33.0	41.0
19	37.723362496190916	39.0	36.0	41.0	33.0	41.0
20	37.67977859216372	39.0	36.0	41.0	33.0	41.0
21	37.54768167310869	39.0	36.0	41.0	33.0	41.0
22	37.537389135699506	39.0	35.0	41.0	33.0	41.0
23	37.47066606790589	39.0	35.0	41.0	33.0	41.0
24	37.41511964523424	39.0	35.0	41.0	33.0	41.0
25	37.22051169989254	39.0	35.0	41.0	32.0	41.0
26	37.19180726852817	39.0	35.0	41.0	32.0	41.0
27	37.16025605042421	39.0	35.0	41.0	32.0	41.0
28	37.051919776747766	39.0	35.0	41.0	32.0	41.0
29	36.98489398726564	39.0	35.0	41.0	32.0	41.0
30	36.881004314285256	39.0	35.0	40.0	31.0	41.0
31	36.828019999679235	39.0	35.0	40.0	31.0	41.0
32	36.70497265480906	39.0	35.0	40.0	31.0	41.0
33	36.56213813731937	39.0	35.0	40.0	31.0	41.0
34	36.468204198809964	39.0	35.0	40.0	30.0	41.0
35	36.39764598803548	39.0	35.0	40.0	30.0	41.0
36	36.26609436897564	38.0	35.0	40.0	30.0	41.0
37	36.19664881076486	38.0	35.0	40.0	30.0	41.0
38	36.082526743757114	38.0	35.0	40.0	30.0	41.0
39	35.98429255344742	38.0	35.0	40.0	30.0	41.0
40	35.89953048066591	38.0	35.0	40.0	29.0	41.0
41	35.78544449968725	38.0	35.0	40.0	29.0	41.0
42	35.727841173357284	38.0	35.0	40.0	29.0	41.0
43	35.655781382816635	38.0	35.0	40.0	29.0	41.0
44	35.56255112187455	38.0	34.0	40.0	29.0	41.0
45	35.504955814662154	38.0	34.0	40.0	28.0	41.0
46	34.53515380667511	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	18.0
11	15.0
12	11.0
13	10.0
14	14.0
15	12.0
16	31.0
17	44.0
18	129.0
19	229.0
20	413.0
21	666.0
22	1104.0
23	1527.0
24	2109.0
25	2824.0
26	3771.0
27	4913.0
28	6165.0
29	7590.0
30	9610.0
31	12384.0
32	16781.0
33	23050.0
34	36175.0
35	48280.0
36	50786.0
37	70010.0
38	100039.0
39	100080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.13557922086254	21.94190951227727	20.38680213629292	23.535709130567273
2	23.630936151785857	29.431364372664433	23.391565492133246	23.546133983416464
3	27.76880082115764	28.085555965421566	23.160013472117527	20.985629741303267
4	25.047312793700183	28.966255553238923	22.24683645811615	23.739595194944748
5	21.087873490401115	33.49264646918253	20.96177286651377	24.457707173902584
6	19.868165707045595	41.11461724751808	21.309602091385866	17.707614954050456
7	81.08570832865551	7.009109717566679	7.63840996936697	4.266771984410835
8	80.45660855479463	7.267525781463008	7.30742089140511	4.968444772337252
9	73.68105563663775	9.421861718336515	10.368117592340138	6.528965052685602
10	36.31818254719251	27.885078026014014	17.161713525043705	18.63502590174977
11	29.12563551506792	28.27560905197992	22.730389247967153	19.868366184985003
12	24.69527353210053	23.55615788038684	30.657286972141584	21.091281615371045
13	22.422856088915978	29.046847684880756	29.830114994146044	18.700181232057226
14	20.281150262225147	29.346161248416223	28.33535147792337	22.037337011435262
15	18.732658658241245	27.344188545492454	33.33767702202049	20.5854757742458
16	20.782345110744014	26.284863113662972	30.365992526182417	22.566799249410597
17	20.393417908293372	25.563543487674618	27.191624833603314	26.8514137704287
18	22.456736860675854	25.685634552773813	28.394692947988005	23.46293563856233
19	24.16120030151882	27.74995589485333	25.831983448541322	22.256860355086527
20	25.136926432615354	26.274037304934968	25.690245545380186	22.898790717069495
21	25.248993600744175	28.743725040496543	25.76041282417283	20.246868534586454
22	23.37011435261664	25.55572484803772	25.83519109557184	25.238969703773794
23	21.739627271415056	27.96186107680711	26.208481018748696	24.09003063302914
24	21.574433449343232	26.04248528491925	29.336337829385258	23.046743436352262
25	21.23161617295633	28.372640374653173	26.42800436239996	23.967739089990538
26	19.758905230068482	29.08132989045885	27.181400458693528	23.978364420779137
27	21.75045308014306	28.33374765440811	26.211287709900404	23.70451155554843
28	20.40905518756716	27.743941556671103	28.699619893826885	23.147383361934853
29	20.944732241664127	28.309690301679204	26.797084248849256	23.948493207807413
30	21.247654408108932	29.305464226716495	25.816346169267533	23.630535195907044
31	22.54815480104569	29.27719683726003	23.72876938621674	24.445878975477537
32	23.173445494057834	28.64108033551988	24.982157463392728	23.203316707029558
33	22.45413064746355	29.5287966512165	23.642764350210904	24.374308351109043
34	19.71760677455053	28.508764895510897	26.367860980577696	25.405767349360875
35	22.146196532533562	28.062701480329107	24.823779891260767	24.967322095876572
36	23.908397619925903	28.762569966800854	24.967322095876572	22.361710317396675
37	22.04575708489038	29.904492309666242	25.441051466696607	22.608699138746772
38	21.39721095090696	30.768151272633958	24.54611794518131	23.288519831277764
39	21.925871277124664	27.671769498484387	25.939038668184956	24.463320556205996
40	23.526487145354523	26.208881974627513	26.997161232377987	23.267469647639974
41	20.594096325640326	26.20747862905166	27.768399865278827	25.43002518002919
42	21.916047858093695	26.227526422992415	28.153117031001905	23.70330868791198
43	21.487426023640356	25.958485028307486	28.194615964459267	24.359472983592887
44	21.437306538788473	27.25698064185017	26.782048403393688	24.523664415967666
45	22.04475469519334	27.039261599653575	26.078771791952015	24.83721191320107
46	22.9839938413177	27.97890170165675	25.797701720902634	23.239402736122916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4495.0
1	4422.0
2	4349.0
3	3741.0
4	3133.0
5	2337.5
6	1542.0
7	1542.0
8	1481.0
9	1420.0
10	1403.0
11	1386.0
12	1378.0
13	1370.0
14	1401.0
15	1432.0
16	1566.5
17	1701.0
18	1627.5
19	1554.0
20	1554.0
21	1681.5
22	1809.0
23	2052.0
24	2295.0
25	2991.0
26	3687.0
27	4160.0
28	4633.0
29	5669.0
30	6705.0
31	6705.0
32	7461.5
33	8218.0
34	9922.5
35	11627.0
36	12163.5
37	12700.0
38	15621.5
39	18543.0
40	21912.0
41	25281.0
42	29975.0
43	34669.0
44	34669.0
45	40659.5
46	46650.0
47	47817.5
48	48985.0
49	45300.5
50	41616.0
51	38470.0
52	35324.0
53	32945.0
54	30566.0
55	28317.0
56	26068.0
57	26068.0
58	25342.0
59	24616.0
60	23614.5
61	22613.0
62	20946.5
63	19280.0
64	16888.5
65	14497.0
66	13276.5
67	12056.0
68	10230.0
69	8404.0
70	8404.0
71	6902.0
72	5400.0
73	4738.0
74	4076.0
75	3313.5
76	2551.0
77	2176.0
78	1801.0
79	1506.0
80	1211.0
81	1211.0
82	776.5
83	342.0
84	216.5
85	91.0
86	86.5
87	82.0
88	48.5
89	15.0
90	12.5
91	10.0
92	7.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	498808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.830797714559164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.76365390668211	22.302594360915453
2	9.538584969874616	5.6908719743892835
3	3.6121982732552564	3.232642679830724
4	1.9231489091563088	2.294762643360679
5	1.192415608184814	1.7785354399722115
6	0.8339675320603543	1.492675004964154
7	0.6487022249881377	1.354591339542391
8	0.5133881557484448	1.2251822578545961
9	0.4302487252762926	1.1551196421597623
>10	5.857392692679894	41.84184961653639
>50	0.6278616613214575	11.770873347056764
>100	0.049603789260245015	2.7060643909627653
>500	0.004756527737283769	1.072493385212284
>1k	0.0040770237748146585	2.081743917242517
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4010	0.8039165370242659	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1632	0.3271799971131177	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1235	0.24759025516832128	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	1163	0.23315584353097782	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	1143	0.2291462847428269	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1087	0.21791952013600424	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	903	0.18103157928501548	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCG	895	0.17942775576975512	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	835	0.16739907940530224	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTA	745	0.14935606485862296	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCG	723	0.14494555019165692	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC	661	0.13251591794838896	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGT	529	0.10605282994659267	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.014338182226428E-4	0.0	0.0	0.18383827043672113	0.0
2	6.014338182226428E-4	0.0	0.0	0.8578451027248961	0.0
3	6.014338182226428E-4	0.0	0.0	1.086189475710093	0.0
4	6.014338182226428E-4	0.0	0.0	1.430009141794037	0.0
5	6.014338182226428E-4	0.0	0.0	2.4676829561675033	0.0
6	6.014338182226428E-4	0.0	0.0	3.4798960722362113	0.0
7	6.014338182226428E-4	0.0	0.0	4.217454411316579	0.0
8	6.014338182226428E-4	0.0	0.0	5.600150759410434	0.0
9	6.014338182226428E-4	0.0	0.0	6.200983143814855	0.0
10	6.014338182226428E-4	0.0	0.0	7.498075411781688	0.0
11	6.014338182226428E-4	0.0	0.0	8.71577841574313	0.0
12	6.014338182226428E-4	0.0	0.0	9.873137559942904	0.0
13	6.014338182226428E-4	0.0	0.0	10.308174688457282	0.0
14	6.014338182226428E-4	0.0	0.0	10.49882920883386	0.0
15	6.014338182226428E-4	0.0	0.0	10.80776571346089	0.0
16	6.014338182226428E-4	0.0	0.0	11.4773620310821	0.0
17	6.014338182226428E-4	0.0	0.0	12.216925149556543	0.0
18	6.014338182226428E-4	0.0	0.0	13.229338743564659	0.0
19	6.014338182226428E-4	0.0	0.0	13.65515388686629	0.0
20	6.014338182226428E-4	0.0	0.0	14.096004875623487	0.0
21	6.014338182226428E-4	0.0	0.0	14.540263989350612	0.0
22	6.014338182226428E-4	0.0	0.0	15.016399095443537	0.0
23	6.014338182226428E-4	0.0	0.0	15.51578964250774	0.0
24	6.014338182226428E-4	0.0	0.0	15.875848021683694	0.0
25	6.014338182226428E-4	0.0	0.0	16.209443312857854	0.0
26	8.019117576301903E-4	0.0	0.0	16.51396930281792	0.0
27	0.0010023896970377379	0.0	0.0	16.8429536013857	0.0
28	0.0010023896970377379	0.0	0.0	17.171536944074674	0.0
29	0.0010023896970377379	0.0	0.0	17.543223043736266	0.0
30	0.0010023896970377379	0.0	0.0	17.98267068691761	0.0
31	0.0010023896970377379	0.0	0.0	18.342127632275343	0.0
32	0.0010023896970377379	0.0	0.0	18.697775496784335	0.0
33	0.0010023896970377379	0.0	0.0	19.0421965966865	0.0
34	0.0010023896970377379	0.0	0.0	19.37238376289073	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	0.0012550856	40.0	24
GTCATAC	25	7.7883014E-5	40.0	39
CGTGGAA	20	0.0012550856	40.0	10
TACCCGT	20	0.0012550856	40.0	35
CTCCGTA	20	0.0012550856	40.0	14
GGTCGTT	20	0.0012550856	40.0	8
GGTGTAC	25	7.7883014E-5	40.0	8
TTGTGCG	25	7.7883014E-5	40.0	1
CAACGCG	25	7.7883014E-5	40.0	1
ACGCATT	115	0.0	40.0	17
GACACGC	20	0.0012550856	40.0	28
ACGCAAT	20	0.0012550856	40.0	31
GTTTACG	40	1.9244908E-8	40.0	1
GACGTTT	30	4.8619677E-6	40.0	28
GACGTAG	25	7.7883014E-5	40.0	1
ATTACGC	20	0.0012550856	40.0	14
ATTACCG	20	0.0012550856	40.0	20
CGCGATG	20	0.0012550856	40.0	40
GCGCAAT	30	4.8619677E-6	40.0	19
AGTATAT	20	0.0012550856	40.0	28
>>END_MODULE