FastQCFastQC Report
Sat 18 Jun 2016
SRR3553761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296478
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG59160.456313180786716No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTA42570.3283511174119422No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40680.31377316082494267No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCG39230.30258901423703294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30450.23486707834610385No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT30050.23178179652874942No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT26770.20648248562644334No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGT15250.11762636928663657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG401.9286745E-840.0000041
ATACCCG200.00125582840.0000041
CCCGATA200.00125582840.00000440
TATTGCG353.0567753E-740.01
CAATACG257.7952085E-540.01
TACGAGG2300.036.521742
ATCGTAG503.425157E-936.01
TAGTGCG503.425157E-936.01
CGTAAGG2300.035.6521762
ATTAACG455.4435077E-835.5555531
CTAACGG1350.035.5555532
ATAGCGG2200.035.4545442
GTATGCG408.701063E-735.0000041
TATAGCG800.035.0000041
ACGTAAG408.701063E-735.0000041
ACTACGG1150.034.7826082
TACGAAC351.40120555E-534.28571318
CATCGTT351.40120555E-534.28571336
CTTACCG351.40120555E-534.2857131
AACGGGA4450.034.1573074