FastQCFastQC Report
Sat 18 Jun 2016
SRR3553760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836565
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49140.587402054831364No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT28590.3417546753689193No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT21680.2591549969219367No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20670.24708181671478008No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19410.23202022556525792No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT18090.21624141578956807No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG17830.21313346840950792No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTA14680.17547949053570255No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCG12950.15479968681453324No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11210.13400034665566932No Hit
GCCCAGGGGAATGATACCTGTCTCTTATACACATCTGACGCATGCC10850.129697034898663No Hit
AAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG9500.11355961580988924No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA8660.10351855504354116No Hit
GCCCAGGGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTA8480.10136689916503798No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGT8430.10076921697656488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTG304.865922E-640.0000041
CTTTACG304.865922E-640.0000041
ACAACGA700.040.013
TCGCAAG200.001255572940.01
CTATACG353.0550473E-740.01
GAATACG257.7928315E-540.01
ATCCGTA200.001255572940.03
CGTTACG200.001255572940.023
GCCGATT257.7928315E-540.09
TCGGACG257.7928315E-540.01
TACGCCG257.7928315E-540.01
TACGCAA257.7928315E-540.015
TCCGTAG200.001255572940.04
CAATGCG555.456968E-1240.01
CGGTCTA353.0550473E-740.031
ATAACGC200.001255572940.011
TTACCGC200.001255572940.021
TATCGAT257.7928315E-540.019
CTAGGCG257.7928315E-540.01
GTACGTC200.001255572940.016
Produced by FastQC (version 0.11.5)