##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553760_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 836565 Sequences flagged as poor quality 0 Sequence length 46 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.686390178886278 31.0 31.0 34.0 30.0 34.0 2 31.829175258348126 31.0 31.0 34.0 30.0 34.0 3 31.824112890211758 33.0 31.0 34.0 30.0 34.0 4 35.58255963374035 37.0 35.0 37.0 33.0 37.0 5 35.375587073329626 37.0 35.0 37.0 33.0 37.0 6 35.386357306365916 37.0 35.0 37.0 33.0 37.0 7 35.74637117259269 37.0 35.0 37.0 35.0 37.0 8 35.517742195764825 37.0 35.0 37.0 33.0 37.0 9 37.27108114731073 39.0 37.0 39.0 34.0 39.0 10 36.68919211298584 39.0 37.0 39.0 32.0 39.0 11 36.66351807689779 39.0 35.0 39.0 32.0 39.0 12 36.63536724582071 39.0 35.0 39.0 32.0 39.0 13 34.87550758159856 38.0 35.0 39.0 30.0 39.0 14 36.59417140329801 39.0 36.0 41.0 29.0 41.0 15 37.246871432584435 39.0 36.0 41.0 32.0 41.0 16 37.527429428675596 39.0 36.0 41.0 32.0 41.0 17 37.573242963786434 39.0 36.0 41.0 33.0 41.0 18 37.60186596379241 39.0 36.0 41.0 33.0 41.0 19 37.57488778516912 39.0 36.0 41.0 33.0 41.0 20 37.51173668513505 39.0 36.0 41.0 32.0 41.0 21 37.40004183775319 39.0 35.0 41.0 32.0 41.0 22 37.40163286773891 39.0 35.0 41.0 32.0 41.0 23 37.325608888729505 39.0 35.0 41.0 32.0 41.0 24 37.287174337917556 39.0 35.0 41.0 32.0 41.0 25 37.050775492639545 39.0 35.0 40.0 31.0 41.0 26 37.044120899153086 39.0 35.0 40.0 32.0 41.0 27 37.02426589685201 39.0 35.0 40.0 31.0 41.0 28 36.8884270797846 39.0 35.0 40.0 31.0 41.0 29 36.80881939837311 39.0 35.0 40.0 31.0 41.0 30 36.71153108246221 39.0 35.0 40.0 31.0 41.0 31 36.68994041108581 39.0 35.0 40.0 31.0 41.0 32 36.585514574480165 39.0 35.0 40.0 31.0 41.0 33 36.53569059188467 39.0 35.0 40.0 30.0 41.0 34 36.44525529994681 39.0 35.0 40.0 30.0 41.0 35 36.40286767914029 39.0 35.0 40.0 30.0 41.0 36 36.27821269118359 38.0 35.0 40.0 30.0 41.0 37 36.26321325898167 38.0 35.0 40.0 30.0 41.0 38 36.18929909809758 38.0 35.0 40.0 30.0 41.0 39 36.07832386007065 38.0 35.0 40.0 30.0 41.0 40 35.982015742948846 38.0 35.0 40.0 30.0 41.0 41 35.841739733314206 38.0 35.0 40.0 29.0 41.0 42 35.76600981394154 38.0 35.0 40.0 29.0 41.0 43 35.72055727887253 38.0 35.0 40.0 29.0 41.0 44 35.65496524478074 38.0 35.0 40.0 29.0 41.0 45 35.61608243232743 38.0 34.0 40.0 29.0 41.0 46 34.61072480918996 37.0 33.0 40.0 25.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 21.0 10 28.0 11 23.0 12 14.0 13 10.0 14 22.0 15 18.0 16 46.0 17 102.0 18 201.0 19 352.0 20 660.0 21 1099.0 22 1883.0 23 2889.0 24 3703.0 25 5004.0 26 6433.0 27 8257.0 28 10634.0 29 13654.0 30 17123.0 31 22607.0 32 29787.0 33 40808.0 34 61141.0 35 78576.0 36 85796.0 37 118783.0 38 169482.0 39 157403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.705450263876685 21.517515076533204 20.489382175921776 24.287652483668335 2 23.07890002570033 28.46270164302834 23.82110176734623 24.637296563925098 3 27.32698594849175 27.549801868354525 22.75770561761489 22.36550656553884 4 24.94928666630806 28.129194981860344 21.823050211280652 25.098468140550946 5 21.28513624165486 33.51825620244691 20.140096704978095 25.056510850920134 6 20.490936149611805 40.18803081649364 20.853729238014978 18.467303795879577 7 81.3488491629461 6.151225547327464 7.980611189805932 4.519314099920508 8 80.51962489465852 6.189596743827437 7.570362135637995 5.720416225876053 9 73.78553967713208 8.153939024463131 11.127049302803727 6.933471995601059 10 35.148374603288445 27.03950081583619 18.249388869962285 19.562735710913078 11 28.88299175796262 27.779670438041276 23.22246328737157 20.114874516624532 12 24.662757825154053 22.820581783842258 31.42660761566645 21.09005277533724 13 23.091570888095962 27.20565646423171 30.578018444472338 19.12475420319999 14 19.930310256824036 29.803063718898116 28.22303108545062 22.043594938827226 15 18.077136863244338 27.396197545916934 34.5361089694166 19.990556621422126 16 20.608440467865616 25.356666845971322 30.599415466819675 23.435477219343387 17 19.690161553495543 25.75986325031528 27.510115771039906 27.03985942514927 18 22.010124736272736 24.979648921482493 29.41911268102299 23.591113661221783 19 23.600078894048877 27.691930692773425 26.714481241744515 21.993509171433182 20 25.184892985004154 25.515291698792087 26.527526253190132 22.772289063013634 21 24.735316442834687 27.861672434299784 26.362685505609246 21.04032561725628 22 22.099657528106004 26.315707685595264 25.70953841004584 25.87509637625289 23 21.1754017918512 28.22972512596152 25.951241087064364 24.643631995122913 24 21.62712998989917 25.92566028939772 28.135530413058163 24.311679307644955 25 21.259674980425906 28.800511615953333 26.406435841805475 23.53337756181528 26 19.74359434114504 28.79692552282249 27.46230119596206 23.997178940070405 27 21.763042919557954 27.93841482729973 26.543305062965818 23.755237190176494 28 20.09467285865414 27.542271072779762 28.579130133342893 23.783925935223206 29 21.047497803517956 27.964473770717156 25.698182448464852 25.28984597730003 30 21.607884623430337 27.323758464673993 27.496369080705023 23.571987831190643 31 23.98665973355328 27.113254797893767 24.89238732196542 24.007698146587533 32 23.774721629520716 27.731975399401122 24.52636674974449 23.96693622133367 33 22.69925229958222 25.393723141656654 25.684316221692278 26.222708337068845 34 21.930991614518895 25.554499650355922 26.99383789663684 25.52067083848834 35 22.023273744419143 24.660845242150938 26.93012497534561 26.385756038084306 36 23.72666798156748 26.399383191981496 27.113135261456073 22.76081356499495 37 22.97609868928296 25.354037044342043 28.622880469539126 23.04698379683587 38 23.01327452140599 25.69399867314554 26.395438489537575 24.8972883159109 39 22.20580588477883 23.941474960104713 28.154417170213907 25.69830198490255 40 23.69176334176065 24.125919683467515 28.97192686760742 23.210390107164418 41 21.15029913993533 24.281914734658994 29.53494348914908 25.032842636256596 42 22.65406752613365 25.513857261539748 28.631726165928526 23.20034904639807 43 21.67183661759696 24.050611727719904 29.713889536377923 24.563662118305214 44 22.6174893761991 24.130581604537603 27.78552772348831 25.466401295774983 45 21.772366761698134 24.345627655950224 27.066276977879784 26.815728604471857 46 24.126517365655985 24.98012706723327 27.086359099412476 23.806996467698266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5558.0 1 5344.0 2 5130.0 3 4913.5 4 4697.0 5 3414.5 6 2132.0 7 2132.0 8 2077.5 9 2023.0 10 2079.0 11 2135.0 12 2114.5 13 2094.0 14 2211.5 15 2329.0 16 2251.0 17 2173.0 18 2180.0 19 2187.0 20 2187.0 21 2467.0 22 2747.0 23 3365.5 24 3984.0 25 4615.5 26 5247.0 27 6178.5 28 7110.0 29 8090.0 30 9070.0 31 9070.0 32 10562.5 33 12055.0 34 14608.5 35 17162.0 36 19409.0 37 21656.0 38 24203.0 39 26750.0 40 31855.0 41 36960.0 42 43914.0 43 50868.0 44 50868.0 45 59658.5 46 68449.0 47 77638.5 48 86828.0 49 87763.0 50 88698.0 51 83146.0 52 77594.0 53 67826.5 54 58059.0 55 52800.0 56 47541.0 57 47541.0 58 44527.5 59 41514.0 60 39073.5 61 36633.0 62 33267.5 63 29902.0 64 26905.5 65 23909.0 66 20471.0 67 17033.0 68 15230.5 69 13428.0 70 13428.0 71 11240.0 72 9052.0 73 7377.0 74 5702.0 75 4775.0 76 3848.0 77 2954.5 78 2061.0 79 1729.0 80 1397.0 81 1397.0 82 941.5 83 486.0 84 320.0 85 154.0 86 129.5 87 105.0 88 96.5 89 88.0 90 50.0 91 12.0 92 7.0 93 2.0 94 2.0 95 2.5 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 836565.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.46727654372848 #Duplication Level Percentage of deduplicated Percentage of total 1 73.81184029326208 21.750339101230743 2 10.516122971757543 6.1976300755327065 3 4.037495773590879 3.569220135136122 4 2.0389285927420713 2.4032669078098303 5 1.2034167191001925 1.7730706629535895 6 0.8160120875877348 1.442739230878378 7 0.626117342060604 1.291498100712684 8 0.4974668655415887 1.172719495860463 9 0.42552367779313366 1.1285121500491215 >10 5.121107658550048 35.997853706452766 >50 0.8241255140931664 15.65453492723977 >100 0.07207026464687515 3.7688131984736772 >500 0.005293296273499304 1.1489654958003226 >1k 0.004478943000653257 2.700836811869806 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4914 0.587402054831364 No Hit CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT 2859 0.3417546753689193 No Hit GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT 2168 0.2591549969219367 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2067 0.24708181671478008 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1941 0.23202022556525792 No Hit CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT 1809 0.21624141578956807 No Hit GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG 1783 0.21313346840950792 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTA 1468 0.17547949053570255 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCG 1295 0.15479968681453324 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1121 0.13400034665566932 No Hit GCCCAGGGGAATGATACCTGTCTCTTATACACATCTGACGCATGCC 1085 0.129697034898663 No Hit AAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG 950 0.11355961580988924 No Hit CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA 866 0.10351855504354116 No Hit GCCCAGGGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTA 848 0.10136689916503798 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGT 843 0.10076921697656488 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.23441095431915032 0.0 2 0.0 0.0 0.0 1.063037540418258 0.0 3 0.0 0.0 0.0 1.3763425436158576 0.0 4 0.0 0.0 0.0 1.8041634541249036 0.0 5 0.0 0.0 0.0 3.1629341413996523 0.0 6 0.0 0.0 0.0 4.476998200976612 0.0 7 0.0 0.0 0.0 5.427671489961928 0.0 8 0.0 0.0 0.0 7.144573344569759 0.0 9 0.0 0.0 0.0 7.856651903916611 0.0 10 0.0 0.0 0.0 9.455810367395241 0.0 11 0.0 0.0 0.0 10.862993311936311 0.0 12 0.0 0.0 0.0 12.25774446695714 0.0 13 1.1953643769462027E-4 0.0 0.0 12.812991220048652 0.0 14 1.1953643769462027E-4 0.0 0.0 13.04118627960768 0.0 15 1.1953643769462027E-4 0.0 0.0 13.506183022239755 0.0 16 1.1953643769462027E-4 0.0 0.0 14.317237751997753 0.0 17 1.1953643769462027E-4 0.0 0.0 15.258826271718277 0.0 18 1.1953643769462027E-4 0.0 0.0 16.57420523210988 0.0 19 1.1953643769462027E-4 0.0 0.0 17.067053964724796 0.0 20 1.1953643769462027E-4 0.0 0.0 17.644414958789813 0.0 21 1.1953643769462027E-4 0.0 0.0 18.201215685571356 0.0 22 1.1953643769462027E-4 0.0 0.0 18.787063766712688 0.0 23 1.1953643769462027E-4 0.0 0.0 19.38115986205495 0.0 24 4.781457507784811E-4 0.0 0.0 19.86050097721038 0.0 25 5.976821884731013E-4 0.0 0.0 20.26752254756056 0.0 26 5.976821884731013E-4 0.0 0.0 20.61262424318493 0.0 27 5.976821884731013E-4 0.0 0.0 21.0599295930382 0.0 28 5.976821884731013E-4 0.0 0.0 21.47914388003323 0.0 29 7.172186261677216E-4 0.0 0.0 21.873853197300868 0.0 30 7.172186261677216E-4 0.0 0.0 22.432925116398607 0.0 31 7.172186261677216E-4 0.0 0.0 22.827873506541632 0.0 32 7.172186261677216E-4 0.0 0.0 23.248163621475918 0.0 33 7.172186261677216E-4 0.0 0.0 23.625540155277832 0.0 34 8.367550638623419E-4 0.0 0.0 24.013674968472266 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGATTG 30 4.865922E-6 40.000004 1 CTTTACG 30 4.865922E-6 40.000004 1 ACAACGA 70 0.0 40.0 13 TCGCAAG 20 0.0012555729 40.0 1 CTATACG 35 3.0550473E-7 40.0 1 GAATACG 25 7.7928315E-5 40.0 1 ATCCGTA 20 0.0012555729 40.0 3 CGTTACG 20 0.0012555729 40.0 23 GCCGATT 25 7.7928315E-5 40.0 9 TCGGACG 25 7.7928315E-5 40.0 1 TACGCCG 25 7.7928315E-5 40.0 1 TACGCAA 25 7.7928315E-5 40.0 15 TCCGTAG 20 0.0012555729 40.0 4 CAATGCG 55 5.456968E-12 40.0 1 CGGTCTA 35 3.0550473E-7 40.0 31 ATAACGC 20 0.0012555729 40.0 11 TTACCGC 20 0.0012555729 40.0 21 TATCGAT 25 7.7928315E-5 40.0 19 CTAGGCG 25 7.7928315E-5 40.0 1 GTACGTC 20 0.0012555729 40.0 16 >>END_MODULE