FastQCFastQC Report
Sat 18 Jun 2016
SRR3553755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723953
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23360.3226728806980564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21540.29753312715051944No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC200.001255461140.09
TTATACG200.001255461140.01
GTTACCA200.001255461140.029
GTTCGAC200.001255461140.021
CATACCG200.001255461140.01
CGGTCTA200.001255461140.031
ACGTAAG257.791792E-540.01
ACCAACG200.001255461140.029
CCAACGT257.791792E-540.030
TGTACGC200.001255461140.030
CTAACGG950.037.8947372
TATACGG850.037.647062
AACGGGA3300.036.3636364
CATACGA1000.036.018
GCGATCG503.421519E-936.09
AATGCGG950.035.7894742
AACGGGC1650.035.1515164
CTTAACG408.6940054E-735.01
CCGTGCG408.6940054E-735.01
TAATGCG408.6940054E-735.01
Produced by FastQC (version 0.11.5)