##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553755_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723953
Sequences flagged as poor quality	0
Sequence length	46
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.892748562406677	33.0	31.0	34.0	30.0	34.0
2	32.04010619473916	33.0	31.0	34.0	30.0	34.0
3	32.012246651371015	33.0	31.0	34.0	30.0	34.0
4	35.73617486218028	37.0	35.0	37.0	35.0	37.0
5	35.54961440867017	37.0	35.0	37.0	33.0	37.0
6	35.54002124447305	37.0	35.0	37.0	33.0	37.0
7	35.83343946361159	37.0	35.0	37.0	35.0	37.0
8	35.65906488404634	37.0	35.0	37.0	35.0	37.0
9	37.42520025471267	39.0	37.0	39.0	35.0	39.0
10	36.87200826572996	39.0	37.0	39.0	32.0	39.0
11	36.82654398835283	39.0	37.0	39.0	32.0	39.0
12	36.77802840792151	39.0	35.0	39.0	33.0	39.0
13	35.12644743512355	39.0	35.0	39.0	30.0	39.0
14	36.924711963345686	40.0	36.0	41.0	30.0	41.0
15	37.57153986515699	40.0	36.0	41.0	32.0	41.0
16	37.77564013133449	40.0	36.0	41.0	33.0	41.0
17	37.82105053781115	40.0	37.0	41.0	33.0	41.0
18	37.8600226810304	40.0	37.0	41.0	33.0	41.0
19	37.82906763284357	40.0	37.0	41.0	33.0	41.0
20	37.799470407609334	40.0	36.0	41.0	33.0	41.0
21	37.68725594064808	40.0	36.0	41.0	33.0	41.0
22	37.670241023933876	40.0	36.0	41.0	33.0	41.0
23	37.59946571117186	39.0	36.0	41.0	33.0	41.0
24	37.51759437422042	39.0	36.0	41.0	32.0	41.0
25	37.32788454499118	39.0	35.0	41.0	32.0	41.0
26	37.31431874721149	39.0	35.0	41.0	32.0	41.0
27	37.29366409145345	39.0	35.0	41.0	32.0	41.0
28	37.18185987211877	39.0	35.0	41.0	32.0	41.0
29	37.1300015332487	39.0	35.0	41.0	32.0	41.0
30	37.00843010526926	39.0	35.0	41.0	31.0	41.0
31	36.95709389974211	39.0	35.0	41.0	31.0	41.0
32	36.83868151661779	39.0	35.0	41.0	31.0	41.0
33	36.734796319650584	39.0	35.0	41.0	31.0	41.0
34	36.6526915421305	39.0	35.0	41.0	31.0	41.0
35	36.5613292575623	39.0	35.0	41.0	30.0	41.0
36	36.41856032090481	39.0	35.0	40.0	30.0	41.0
37	36.36982373165109	39.0	35.0	40.0	30.0	41.0
38	36.25919500299052	39.0	35.0	40.0	30.0	41.0
39	36.144286991006325	38.0	35.0	40.0	30.0	41.0
40	36.04012277040084	38.0	35.0	40.0	29.0	41.0
41	35.938280523735656	38.0	35.0	40.0	29.0	41.0
42	35.843148657440466	38.0	35.0	40.0	29.0	41.0
43	35.776155358151705	38.0	35.0	40.0	29.0	41.0
44	35.661081589550705	38.0	35.0	40.0	29.0	41.0
45	35.57544619609284	38.0	34.0	40.0	28.0	41.0
46	34.56946100092133	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	19.0
10	23.0
11	23.0
12	19.0
13	18.0
14	18.0
15	26.0
16	70.0
17	97.0
18	189.0
19	352.0
20	552.0
21	967.0
22	1460.0
23	2118.0
24	2986.0
25	4151.0
26	5555.0
27	7313.0
28	9216.0
29	11553.0
30	13996.0
31	17881.0
32	23113.0
33	31608.0
34	48858.0
35	60897.0
36	70719.0
37	100179.0
38	149424.0
39	160547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.03390413466067	20.79858775362489	19.99231994342174	24.175188168292692
2	24.377963762840956	27.44377052101449	24.36718958274916	23.8110761333954
3	29.067494713054575	26.623137137355602	22.915576011149895	21.393792138439927
4	25.309654079753795	27.954715292291077	21.94728110802773	24.7883495199274
5	22.336809157500557	32.57131333111404	20.06677229046637	25.025105220919038
6	20.74803198550182	39.74180644323596	20.674684682569172	18.83547688869305
7	84.22563343200457	5.612795305772613	6.206342124419679	3.955229137803145
8	84.4625272635102	4.891892153219891	6.439506432047384	4.206074151222524
9	77.89124432110924	7.428106520727175	9.051140060197278	5.629509097966304
10	36.782222050326475	27.625135885893144	16.45977017845081	19.13287188532957
11	30.637762396177653	27.40675154326317	22.172157584815587	19.78332847574359
12	25.708575004178446	24.83862902702247	28.27421117116719	21.178584797631892
13	22.91433283652392	27.539494967214722	28.676723488955773	20.869448707305587
14	21.834014086549818	27.361859126213993	28.317860413590385	22.4862663736458
15	22.32783067409072	25.811205976078554	29.882464745639563	21.978498604191156
16	23.540616586988385	24.677292586673445	29.80138213392306	21.980708692415117
17	23.265046211563458	24.997340987605547	27.052999296915687	24.68461350391531
18	24.536813853938032	24.83282754543458	27.095405364712903	23.534953235914486
19	25.751533594031656	26.915559435488216	25.014469171341236	22.318437799138895
20	28.22531296921209	24.922059857476935	24.70740503872489	22.145222134586085
21	27.416696940270985	26.34729050090268	24.70353738433296	21.53247517449337
22	25.917980863398594	24.550074383281785	24.62922316780233	24.90272158551729
23	24.957006877518292	26.492465671114008	24.998722292745523	23.551805158622173
24	23.306209104734698	25.77252943215927	27.639225198320887	23.282036264785145
25	24.235413072395584	26.822321338539933	25.409522441373955	23.532743147690528
26	23.3658814867816	26.859616577319244	25.779712218887134	23.994789717012015
27	23.8543109842766	25.762445904637453	26.110258538883045	24.27298457220289
28	22.608650009047548	27.365726780605925	26.921360917076107	23.10426229327042
29	22.925935799699705	26.739166769113464	26.25902510245831	24.075872328728522
30	23.598493272353316	25.66920780768917	26.939594144923774	23.792704775033737
31	25.13270889132306	26.474232443266345	24.580877487903223	23.81218117750738
32	24.31994894696203	27.6222351450992	24.584883272809147	23.472932635129627
33	23.48646942550138	27.267377854639737	25.179949527110185	24.0662031927487
34	23.019726418703975	27.42332720494286	26.089124570241438	23.467821806111722
35	23.93857059781505	26.39038722126989	25.99160442735923	23.679437753555824
36	23.641866253748518	29.104099299263904	24.946094566912492	22.307939880075086
37	24.213312190155992	28.212466831410328	24.857138515898132	22.71708246253555
38	23.163658414289326	28.442177876188097	25.962044497363777	22.4321192121588
39	23.851272112968662	26.616368742169726	25.20757562990968	24.324783514951935
40	24.123941747599638	26.37077268828225	27.030760284162092	22.47452527995602
41	21.93761197204791	26.636121405671364	27.081177921771165	24.345088700509564
42	22.901901090264147	27.460622443722176	25.815073630470486	23.82240283554319
43	22.992100315904484	25.982902204977393	26.781158445368693	24.243839033749428
44	23.433427308126355	26.821630685969943	26.183191450273707	23.56175055562999
45	23.4092544681768	25.831787422664178	26.435694029861057	24.323264079297964
46	23.330243814170256	26.543573961293067	25.85665091518372	24.269531309352953
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2471.0
1	2523.0
2	2575.0
3	3620.5
4	4666.0
5	3308.0
6	1950.0
7	1950.0
8	1788.5
9	1627.0
10	1676.0
11	1725.0
12	1761.0
13	1797.0
14	1763.5
15	1730.0
16	1767.0
17	1804.0
18	1908.5
19	2013.0
20	2013.0
21	2157.0
22	2301.0
23	2678.0
24	3055.0
25	4067.0
26	5079.0
27	5784.0
28	6489.0
29	7523.0
30	8557.0
31	8557.0
32	10366.0
33	12175.0
34	14213.5
35	16252.0
36	18399.5
37	20547.0
38	23817.5
39	27088.0
40	30190.0
41	33292.0
42	37091.0
43	40890.0
44	40890.0
45	47058.5
46	53227.0
47	55502.5
48	57778.0
49	56667.0
50	55556.0
51	54791.5
52	54027.0
53	51662.0
54	49297.0
55	48522.0
56	47747.0
57	47747.0
58	45932.0
59	44117.0
60	42565.0
61	41013.0
62	37478.5
63	33944.0
64	30259.0
65	26574.0
66	23115.5
67	19657.0
68	17032.5
69	14408.0
70	14408.0
71	12763.0
72	11118.0
73	9198.5
74	7279.0
75	6040.5
76	4802.0
77	3744.0
78	2686.0
79	2050.5
80	1415.0
81	1415.0
82	1083.5
83	752.0
84	537.5
85	323.0
86	198.5
87	74.0
88	50.0
89	26.0
90	30.0
91	34.0
92	23.0
93	12.0
94	12.0
95	8.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	723953.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.56850651073491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92883629913398	21.268282644205286
2	8.818427853378111	5.214954827941135
3	3.4889006131722042	3.0948474148766802
4	1.9932305417781768	2.3574740100785476
5	1.4187562945069523	2.097525236563748
6	1.1590481951955196	2.0562794463536562
7	0.9643416296567293	1.99598992285544
8	0.7935264723647606	1.8770714131646344
9	0.7422424359873152	1.9752300270931205
>10	8.478528921413329	51.86946651844156
>50	0.17385915931874968	3.1573385180289586
>100	0.03889571488163082	2.3186055215824988
>500	4.68623070863022E-4	0.09477835276175396
>1k	9.37246141726044E-4	0.622156146053034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2336	0.3226728806980564	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2154	0.29753312715051944	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3813051399745563E-4	0.0	0.0	0.04654998321714255	0.0
2	1.3813051399745563E-4	0.0	0.0	0.2316448719737331	0.0
3	1.3813051399745563E-4	0.0	0.0	0.3453262849936391	0.0
4	1.3813051399745563E-4	0.0	0.0	0.474754576609255	0.0
5	1.3813051399745563E-4	0.0	0.0	0.8658020617360519	0.0
6	1.3813051399745563E-4	0.0	0.0	1.547338017799498	0.0
7	1.3813051399745563E-4	0.0	0.0	1.9519222932980456	0.0
8	1.3813051399745563E-4	0.0	0.0	2.7510073167733267	0.0
9	1.3813051399745563E-4	0.0	0.0	3.14440302063808	0.0
10	1.3813051399745563E-4	0.0	0.0	3.7669572472246124	0.0
11	1.3813051399745563E-4	0.0	0.0	4.369482549281514	0.0
12	1.3813051399745563E-4	0.0	0.0	4.883328061352049	0.0
13	1.3813051399745563E-4	0.0	0.0	5.132101117061467	0.0
14	1.3813051399745563E-4	0.0	0.0	5.263877627415039	0.0
15	1.3813051399745563E-4	0.0	0.0	5.414439887672266	0.0
16	2.7626102799491127E-4	0.0	0.0	5.714873755616732	0.0
17	2.7626102799491127E-4	0.0	0.0	6.044729423042656	0.0
18	2.7626102799491127E-4	0.0	0.0	6.446551088261255	0.0
19	2.7626102799491127E-4	0.0	0.0	6.631231585475852	0.0
20	4.143915419923669E-4	0.0	0.0	6.817984040400413	0.0
21	4.143915419923669E-4	0.0	0.0	7.051148348028118	0.0
22	4.143915419923669E-4	0.0	0.0	7.300612056307522	0.0
23	4.143915419923669E-4	0.0	0.0	7.575215518134464	0.0
24	4.143915419923669E-4	0.0	0.0	7.794152382820432	0.0
25	4.143915419923669E-4	0.0	0.0	7.989192668584839	0.0
26	4.143915419923669E-4	0.0	0.0	8.173458774257444	0.0
27	4.143915419923669E-4	0.0	0.0	8.367670276937867	0.0
28	4.143915419923669E-4	0.0	0.0	8.566025695038213	0.0
29	4.143915419923669E-4	0.0	0.0	8.777503511968318	0.0
30	4.143915419923669E-4	0.0	0.0	9.0498968855713	0.0
31	4.143915419923669E-4	0.0	0.0	9.273530187733181	0.0
32	4.143915419923669E-4	0.0	0.0	9.51843558905067	0.0
33	5.525220559898225E-4	0.0	0.0	9.743450196352526	0.0
34	5.525220559898225E-4	0.0	0.0	9.960315103328531	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGAC	20	0.0012554611	40.0	9
TTATACG	20	0.0012554611	40.0	1
GTTACCA	20	0.0012554611	40.0	29
GTTCGAC	20	0.0012554611	40.0	21
CATACCG	20	0.0012554611	40.0	1
CGGTCTA	20	0.0012554611	40.0	31
ACGTAAG	25	7.791792E-5	40.0	1
ACCAACG	20	0.0012554611	40.0	29
CCAACGT	25	7.791792E-5	40.0	30
TGTACGC	20	0.0012554611	40.0	30
CTAACGG	95	0.0	37.894737	2
TATACGG	85	0.0	37.64706	2
AACGGGA	330	0.0	36.363636	4
CATACGA	100	0.0	36.0	18
GCGATCG	50	3.421519E-9	36.0	9
AATGCGG	95	0.0	35.789474	2
AACGGGC	165	0.0	35.151516	4
CTTAACG	40	8.6940054E-7	35.0	1
CCGTGCG	40	8.6940054E-7	35.0	1
TAATGCG	40	8.6940054E-7	35.0	1
>>END_MODULE