FastQCFastQC Report
Sat 18 Jun 2016
SRR3553753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784429
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39100.49845174005550535No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT27970.3565650938453321No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT16270.2074120156190044No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.2009104711834978No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15040.1917318202157238No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT14170.1806409502963302No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG11550.1472408592747081No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA10650.1357675455649906No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTA10350.13194310766175144No Hit
AAAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG9720.12391178806494915No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9130.1163903935219121No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT200.001255524840.00000425
CGTTCGA200.001255524840.00000415
CGTAGGT200.001255524840.00000420
GTATGCG200.001255524840.0000041
ACGTCGT200.001255524840.00000419
TTCGGAT200.001255524840.00000436
AATAGTA200.001255524840.00000432
TGTCGGC200.001255524840.00000412
TACGTTA200.001255524840.00000427
CTATGCG600.040.01
GCCGATA257.79239E-540.09
GCTACGA451.2205419E-940.010
CCGCACG257.79239E-540.01
GTACGAG555.456968E-1240.01
GCTAGCG552.1645974E-1036.3636361
TGTTACG503.421519E-936.01
CGTTAGG950.035.7894742
ATGGGTA1800.035.5555575
GATCGGC455.4387783E-835.5555579
CTACGAA455.4387783E-835.55555711